Carriage of Distinct <i>mcr‐1</i>‐Harboring Plasmids by Unusual Serotypes of <i>Salmonella</i>

Tang, Biao; Chang, Jiang; Zhang, Ling; Liu, Lizhang; Xia, Xiaodong; Hassan, Bachar H.; Xu, Jin; Yang, Hua; Feng, Youjun

doi:10.1002/adbi.201900219

Cited by 28 publications

(28 citation statements)

References 36 publications

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“…The panel of antimicrobial compounds tested included ampicillin (AMP), amoxicillin (AMC), piperacillin (TZP), cefazolin (CFZ), cefoxitin (FOX), ceftriaxone (CRO), cefepime (FEP), aztreonam (ATM), ertapenem (ETP), imipenem (IMP), amikacin (AMK), gentamicin (GEN), tobramycin (TOB), ciprofloxacin (CIP), levofloxacin (LEV), tigecycline (TGC), nitrofurantoin (NIT), and trimethoprim (SXT), as well as testing for extendedspectrum b-lactamase (ESBL). Colistin susceptibility testing was performed by the broth microdilution method using the Gram-negative panel (Tang et al, 2020). The minimum inhibitory concentration results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) documents M100-S26.…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Similar Antimicrobial Resistance of Escherichia coli Strains Isolated from Retail Chickens and Poultry Farms

Tang

et al. 2021

Foodborne Pathogens and Disease

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Antimicrobial resistance (AMR) is a major public health challenge and spreads through humans, animals, and the environment. Many reports show that AMR genes (ARGs) or phenotypes can be transferred from food animals to humans. However, the level and correlation of AMR in different nodes of the poultry meat supply chain are still poorly understood. Herein, 225 Escherichia coli isolates were recovered from chilled chicken samples from markets (123) and chicken fecal samples from farms (102) in Zhejiang Province, China. The dominant sequence types (STs) were ST155 (8.89%), ST48 (7.56%), and ST10 (7.11%), which are common in chicken and fecal samples. Antimicrobial susceptibility testing (AST) analysis showed that the E. coli isolates from fecal samples and retail chickens were resistant to ampicillin (61.77% and 63.42%, respectively) and trimethoprim (56.87% and 52.85%). Moreover, 36.59% of the E. coli isolates from chilled chickens and 39.22% of the isolates from fecal samples were resistant to three or more antimicrobial agents. A total of 59 ARGs were identified in sequenced E. coli genomes, including the mcr-1 gene involved in colistin resistance. The E. coli from farms and markets could be clustered in the same branch according to core single nucleotide polymorphisms. In addition, toxin genes astA and hlyE were also predicted in 86.5% (32/37) and 13.5% (5/37) of the above genomes, respectively. Taken together, these findings demonstrated that E. coli isolates from markets and farms showed similar AMR patterns, suggesting that E. coli strains in markets may originate from farms.

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Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Similar Antimicrobial Resistance of Escherichia coli Strains Isolated from Retail Chickens and Poultry Farms

Tang

et al. 2021

Foodborne Pathogens and Disease

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“…(http://enter obase.warwi ck.ac.uk/speci es/sente rica/search_strai ns?query =st_search) to obtain the allele number and sequence type (ST) number for each isolate and sequence information for newly assigned alleles, and STs were deposited in the MLST database (Almeida et al, 2017;Kotetishvili et al, 2002;Tang et al, 2020).…”

Section: Impactsmentioning

confidence: 99%

Characterization of the emerging multidrug‐resistant Salmonella enterica serotype Kentucky ST314 in China

Wang

Liao

Zhou

et al. 2021

Zoonoses and Public Health

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Salmonella enterica serotype Kentucky (S. Kentucky) is an important Salmonella serotype with multiple sequence types (ST) with a worldwide incidence. We identified 8 STs from 180 strains of S. Kentucky, and ST314 emerged as the most commonly encountered ST. Drug susceptibility testing revealed that ST314 had multiple resistance properties, and 75.5% of the strains were resistant to three or more classes of antimicrobials. The rate of resistance to chloramphenicol, florfenicol, sulfafurazole and tetracycline were greater than 60%. The rates of ST314 resistance to quinolones were as follows: ciprofloxacin, 32.1%; nalidixic acid, 16%; and ofloxacin, 7.5%.Investigating the mechanism of quinolone resistance of ST314 revealed that mutations in the quinolone resistance-determining regions were rare, and resistance mainly occurred due to the resistance genes carried by plasmids. Only 1.9% (2/106) of ST314 strains had mutations in the quinolone resistance-determining regions (QRDR). The drug resistance genes of ST314 were primarily of plasmid-mediated quinolone resistance (PMQR). The detection rate of Salmonella genomic island 1 (SGI1) in ST314 was 12.3%. XbaI-pulsed-field gel electrophoresis revealed that S. enterica Kentucky ST314 was capable of cross-regional and cross-host transmission in China.We found ST314 to be the dominant S. Kentucky ST in China, and it carried multidrug resistance. This is the first report about the emergence of quinolone-resistant S. enterica Kentucky ST314 in China, which is different from previous reports, and the findings of the present study suggest that the mechanism of quinolone resistance in these strains are plasmid-mediated. Notably, plasmids carrying resistance genes may promote the rapid spread of ciprofloxacin resistance.

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“…The emergence and evolution of multi-drug-resistant (MDR, resistance to three or more classes of antimicrobials) bacteria, including gram-negative bacteria, pose a growing threat to public health [1,2].…”

Section: Introductionmentioning

confidence: 99%

Genetic Characterization of Multidrug-Resistant Salmonella Typhimurium Carrying Mcr-1 in China

Zhang¹,

Huang²,

Beibei³

et al. 2021

Preprint

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Background: With the rapid emergence of plasmid mediated polymyxin resistance gene mcr-1, multidrug-resistant Salmonella caused great troubles in clinical treatment and attracted extensive attention. Here we report multidrug-resistant Salmonella strains harboring mcr-1 in China, which are resistant to both polymyxin, traditional antibiotics and even clinically wide-used antibiotics. Methods: We screened 1454 strains of Salmonella collected in our laboratory from 2006 to 2018 from 3 provinces or regions for mcr-1 by PCR. Antimicrobial susceptibility testing was determined. Plasmid conjugation assays were carried out to analysis the transferability of polymyxin resistance. Genetic polymorphism analysis of Salmonella was performed using the PFGE, and the plasmid profiles were characterized by S1-PFGE and southern blotting. The plasmids harboring mcr-1 were sequenced and compared. Results: Eleven S. Typhimurium isolates harboring mcr-1 with polymyxin resistance (MICs 4μg/ml) were identified from intestinal infections and foods in China. All S. Typhimurium isolates were multidrug-resistant to traditional antibiotics and even clinically wide-used antibiotics. Three types of plasmids harboring mcr-1 were recovered (IncHI2, IncX4 and IncI2). Compared with the reference plasmid, IncX4 and IncI2 plasmids had extremely similar typical backbone, and contain only mcr-1 resistance gene. However, IncHI2 were the most diverse type of plasmid due to containing a large MDR region, including blaCTX-M, oqxB, sul, aph, aadA and blaTEM. IncHI2 plasmids were observed to contain only one or no insertion sequence ISApl1 around mcr-1, without forming a circular intermediate. Conclusion: With the horizontal transfer of different types of plasmids, mcr-1 is widely spread worldwide. These prevalent plasmids are responsible for resistance to polymyxin, traditional antibiotics and even clinically wide-used antibiotics resulting from transmission of mcr-1 and other resistance genes. Our studying emphasizes the necessity to jointly monitor its international epidemic and preemptive further upgrade.

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Carriage of Distinct mcr‐1‐Harboring Plasmids by Unusual Serotypes of Salmonella

Cited by 28 publications

References 36 publications

Similar Antimicrobial Resistance of Escherichia coli Strains Isolated from Retail Chickens and Poultry Farms

Similar Antimicrobial Resistance of Escherichia coli Strains Isolated from Retail Chickens and Poultry Farms

Characterization of the emerging multidrug‐resistant Salmonella enterica serotype Kentucky ST314 in China

Genetic Characterization of Multidrug-Resistant Salmonella Typhimurium Carrying Mcr-1 in China

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