Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

Husičková, Vendula; Čekanová, Luboslava; Chromá, M; Htoutou-Sedláková, Miroslava; Hricová, Kristýna; Kolář, Milan

doi:10.5507/bp.2012.039

Cited by 28 publications

(28 citation statements)

References 27 publications

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“…These results are similar to those obtained from patients in other works; Naseer et al reported a prevalence of 3.7% of AmpC among outpatients in Norway [18], and Li et al reported a prevalence of 1.9% [19]. These findings are nearly similar to those reported in the Czech Republic and Denmark (rates were1.1% and 2.4%, respectively) [20,21].…”

Section: Discussionsupporting

confidence: 91%

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Bassyouni

Gaber

Wegdan

2015

J Infect Dev Ctries

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Introduction: Commensal E. coli can be considered a reservoir of genes coding for antibiotic resistance that may be transmitted in hospitals by healthcare workers (HCWs). This study aimed to determine the fecal carriage rate of extended-spectrum β-lactamase (ESBL)-producing E. coli among HCWs. Methodology: Stool samples were collected from 200 HCWs. Phenotypic screening for ESBL and AmpC β-lactamases was performed using disk diffusion and minimum inhibitory concentration methods followed by the combined disks test and double synergy differential test for confirmation. Multiplex polymerase chain reaction (PCR) was used to detect bla SHV , bla TEM , bla CTX-M , and CIT groups for AmpC genes. Results: Of 200 E. coli isolates, 100% were susceptible to imipenem, and 59 (29.5%) were resistant to one or more third-generation cephalosporins. By molecular analysis, 21% (42/200) were colonized by ESBL-producing E. coli, and 3% (6/200) were colonized by AmpCproducing E. coli. The bla SHV gene was the predominant ESBL gene, detected in 81.8% of the resistant E. coli isolates. Conclusions: These findings highlight the increase in fecal carriage of E. coli carrying ESBL and AmpC genes among HCWs, which may be one of the causes of the spread of ESBL-producing bacteria in hospitals and requires sound infection control measures. This is the first study of the fecal carriage rate of E. coli carrying AmpC genes in HCWs.

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Section: Discussionsupporting

confidence: 91%

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Bassyouni

Gaber

Wegdan

2015

J Infect Dev Ctries

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“…Los resultados encontrados difieren a los trabajos realizados por otros autores: Husickova et al en República Checa, reportaron el 3,2% de portadores fecales de enterobacterias productoras de BLEE en sujetos de la comunidad (15) . Adriatahina et al en una unidad pediátrica de Madagascar, realizaron hisopados rectales el primer y el último día de hospitalización, se encontró un 21,2% de productoras de BLEE en las del primer día y al momento del alta se encontró el 57,1% (16) .…”

Section: Discussionunclassified

“…Es importante mencionar que en los últimos años se ha descrito el incremento de enterobacterias productoras de BLEE de la familia CTX-M (19) , en este estudio se encontró que la gran mayoría de los aislados productores de BLEE presentaban en gen bla CTX-M, el grupo de gen dominante en los estudios de portadores fecales de República Checa (15) , España (20) , India (2) , Madagascar (16) , Nigeria (17) , Francia (24) , Guinea-Bissau (25) y Japón (26) poniendo de relieve la importancia de este gen en la difusión global de BLEE. La BLEE tipo CTX-M tiene principalmente actividad sobre la cefotaxima y no sobre la ceftazidima; aunque en los últimos años la emergencia de variantes de CTX-M (CTX-M-15, CTX-M-16, CTX-M-27 y CTX-M-19) están mejorando su actividad frente a ceftazidima.…”

Section: Discussionunclassified

Enterobacterias productoras de Betalactamasas de espectro extendido en muestras fecales en el Instituto Nacional de Salud del Niño, Perú

Aliaga

Sevilla-Andrade

Escalante

2015

Rev Peru Med Exp Salud Publica

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Objetivos. Describir la frecuencia de enterobacterias productoras de betalactamasas de espectro extendido (BLEE) en muestras fecales en el Instituto Nacional de Salud del Niño, Lima, Perú. Materiales y métodos. Se analizaron muestras de heces recibidas entre julio de 2012 y marzo de 2013, se trabajó con colonias sospechosas de ser enterobacterias productoras de BLEE que se desarrollaron en el agar Karmali; se realizó la identificación bioquímica por métodos convencionales y la confirmación del fenotipo BLEE. El análisis genotípico para detectar el gen de betalactamasa de la familia CTX-M se realizó por PCR. Resultados. De 235 muestras fecales se aisló un 64,2% de enterobacterias productoras de BLEE siendo Escherichia coli 86,1%, Klebsiella pneumoniae 7,9%, Salmonella sp. 2,6%, Enterobacter cloacae 2,0% y Proteus mirabilis 1,3%. El 89,1% de las enterobacterias productoras de BLEE presentaron el gen bla CTX-M. Se encontró una alta resistencia al ácido nalidíxico 84,8%, ciprofloxacina 74,2% y trimetoprimsulfametoxazol 81,5%. La resistencia a la amikacina fue de 1,3% y todos los aislados fueron sensibles al imipenem y meropenem. Conclusiones. Se encontró una alta frecuencia de enterobacterias productoras de BLEE en muestras fecales de pacientes ambulatorios atendidos en los consultorios externos y emergencia del Instituto Nacional de Salud del Niño en Perú. Palabras clave: beta-Lactamasa I; Heces; Enterobacterias (fuente: DeCS BIREME). EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL)-PRODUCING ENTEROBACTERIACEAE IN FECAL SAMPLES AT THE NATIONAL INSTITUTE OF CHILD HEALTH, PERU ABSTRACTObjectives. To describe the frequency of extended-spectrum beta-lactamase (ESBL)-producing enterobacteriaceae in fecal samples at the National Institute of Child Health, Lima, Peru. Materials and methods. Stool samples received between July 2012 and March 2013 with colonies suspected to be ESBL-producing enterobacteriaceae that developed in Karmali agar were analyzed. Conventional methods were performed for biochemical identification and the confirmation of the ESBL phenotype. Genotypic analysis to detect the beta-lactamase gene CTX-M family was performed by PCR. Results. Of the 235 fecal samples analyzed, 64.2% of ESBL-producing enterobacteria was isolated being 86.1% Escherichia coli, 7.9% Klebsiella pneumoniae, 2.6% Salmonella sp, 2.0% Enterobacter cloacae, and 1.3% Proteus mirabilis. 89.1% of the ESBL-producing enterobacteria presented the CTX-M gene. We found high resistance to nalidixic acid 84.8%, 74.2% ciprofloxacin and trimethoprim-sulfamethoxazole 81.5%.The resistance to amikacin was 1.3% and all isolates were susceptible to imipenem and meropenem. Conclusions. A high frequency of ESBL-producing enterobacteria was found in fecal samples of outpatients seen in the outpatient and emergency departments of the National Institute of Child Health of Peru.

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“…Considering the probable increase in infections with ESBL-producing bacteria in patients who are already carriers of these bacteria, recent studies on the rates of carriage in the community and the relation between carriage and infections are attracting attention. While rates of 1%-8% carriage in healthy populations were reported in various European countries between 2003 and 2007, the rates were found to be increasing over time (2)(3)(4). On the other hand, up to 50%-60% rates of carriage were reported from countries in Asia and Africa such as Egypt (5) and China (6).…”

Section: Introductionmentioning

confidence: 99%

Fecal carriage of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella spp. in a Turkish community

et al. 2017

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The aim of this study was to determine the prevalence of fecal carriage of extended-spectrum beta-lactamase (ESBL)producing bacteria, enzyme types, and risk factors affecting colonization. Materials and methods:A total of 576 stool samples from outpatients were examined between October 2012 and May 2013. Screening was done with selective EMB plates. ESBL were detected by double-disk synergy and confirmed agar strip gradient methods. Enzyme types were determined by PCR. Results:The prevalence of fecal carriage was found as 30% (173 of 576). Recent use of antibiotics, hospitalization and surgical operation, diabetes, crowded household populations, and old age were associated with higher carriage rates. Of the ESBL-producing bacteria, 87.5% were positive for bla CTX-M genes. Of the bla CTX-M gene-positive isolates, 95.2% were positive for bla CTX-M-1 genes; among these, 82.2% were positive for bla CTX-M-3 and 67.7% were positive for bla CTX-M-15 genes while 62.5% isolates were positive for both bla CTX-M-3 and bla CTX-M-15 genes Conclusions: A high rate (30%) of fecal carriage of ESBL bacteria was found in an adult population. The predominant beta-lactamase enzyme types were CTX-M-3 and CTX-M-15.

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Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

Cited by 28 publications

References 27 publications

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Fecal carriage of extended-spectrum β-lactamase- and AmpC- producing Escherichia coli among healthcare workers

Enterobacterias productoras de Betalactamasas de espectro extendido en muestras fecales en el Instituto Nacional de Salud del Niño, Perú

Fecal carriage of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella spp. in a Turkish community

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