Carriage of Multidrug Resistance Staphylococci in Shelter Dogs in Timisoara, Romania

Dégi, J.; Herman, V.; Iancu, I.; Pascu, C.; Tiana, Florea; Roxana, Dascălu

doi:10.3390/antibiotics10070801

Cited by 15 publications

(18 citation statements)

References 45 publications

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“…In our study, the frequency of S. aureus and MRSA in hunting dogs was 15.4% and 9%, respectively. János et al also reported similar results with a frequency of S. aureus and MRSA of 11.62% and 9.30%, respectively, in kennel dogs from Romania [ 43 ]. Other studies have reported a lower prevalence of MRSA in dogs [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 56%

Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus from Hunters and Hunting Dogs

et al. 2022

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Several studies have showed that a dog-to-human transmission of Staphylococcus aureus occurs. Hunting dogs do not have as much contact with their owners as dogs that live in the same household as the owners; however, these dogs have contact with their owners during hunting activities as well as when hunting game; therefore, we aimed to isolate S. aureus from hunters and their hunting dogs to investigate a possible S. aureus transmission. Nose and mouth samples were collected from 30 hunters and their 78 hunting dogs for staphylococcal isolation. The species identification was performed using MALDI-TOF. The antimicrobial susceptibility profiles were accessed using the Kirby–Bauer method and respective antimicrobial resistance genes were investigated by PCR. Multilocus sequence typing (MLST) and spa- and agr-typing was performed in all S. aureus isolates. S. aureus were detected in 10 (30%) human samples and in 11 (15.4%) dog samples of which 11 and 5 were methicillin-resistant S. aureus (MRSA). Other staphylococci were identified, particularly, S. pseudintermedius. Most S. aureus isolates were resistant to penicillin, erythromycin, and tetracycline. Evidence of a possible transmission of S. aureus between human and dogs was detected in three hunters and their dogs. S. aureus isolates were ascribed to 10 STs and 9 spa-types. A moderate colonization of S. aureus in hunting dogs and their owners was detected in this study. A few dog-to-dog and dog-to-human possible transmissions were identified.

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Section: Discussionmentioning

confidence: 56%

Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus from Hunters and Hunting Dogs

et al. 2022

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“…In another investigation, a total of 12.8% of the screened milk and dairy products origin S. aureus isolates were MDR [ 21 ]. In addition, in context of the One Health approach, the recorded worrying 100% MDR pattern in case of S. aureus strains isolated from shelter dogs with skin lesions from Timișoara Municipility, included in the monitored Banat region in the present study, indirectly suggests the increased AMR trend in the case of this pathogen in Romania [ 31 ]. In contrast, in a study conducted in Poland most of the isolates (47.5%) were found to be resistant to only one antimicrobial [ 11 ].…”

Section: Discussionmentioning

confidence: 87%

“…It is important to mention that the acquiring of VAN resistance in the case of S. aureus can be closely related to the presence of the vanA gene clusters and several mobile genetic elements (e.g., self-transferable or mobilizable plasmids, conjugative transposons) in other Gram-positive bacteria (e.g., Enterococcus faecalis , E. faecium , Clostidium difficile ) [ 34 ]. Until now, VAN resistance has not been reported for food origin S. aureus strains in Romania, but its occurrence has been recently confirmed for strains isolated from shelter dogs with skin lesions [ 31 ]. Further molecular studies focusing on the evidence of the van A gene in a large number of food or animal origin S. aureus isolates are still required to improve our understanding about the demonstrated spreading of VAN resistance by this pathogen in Romania.…”

Section: Discussionmentioning

confidence: 99%

Multidrug Resistant Coagulase-Positive Staphylococcus aureus and Their Enterotoxins Detection in Traditional Cheeses Marketed in Banat Region, Romania

et al. 2021

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The main objectives of the present study were to determine the occurrence of coagulase positive staphylococci (CPS) and to assess the presence and antimicrobial susceptibility profile of Staphylococcus aureus isolates in different raw milk origin (cow and sheep) traditional cheeses marketed in Banat region, Romania. Additionally, the presence of mecA gene in S. aureus isolates and the staphylococcal enterotoxins (SEs) in cheese samples were evaluated. A total of 81.6% (138/169) of the screened samples were positive for CPS. Furthermore, 35.5% (49/138) of the investigated CPS positive cheese samples were contaminated with S. aureus, with an isolation frequency of 46.6% (14/30) in caș, 33.3% (32/96) in telemea, 25% (2/8) in burduf, and 25% (1/4) in urdă assortments, respectively. From the total number of S. aureus isolates, 6.1% (3/49) harbored the mecA gene. Detectable levels of SEs were identified in 4.3% (4/94) of cheese samples with a CPS contamination level higher than 105 log CFU g−1. The expressed antimicrobial susceptibility profile of the tested cheese-origin S. aureus isolates, with the automated Vitek 2 equipment, showed resistance towards amikacin (90.1%, 10 out from 11 tested), enrofloxacin (86.2%, 25/29), ceftiofur (72.7%, 8/11), neomycin (63.6%, 7/11), benzylpenicillin (53.1%, 26/49), kanamycin (41.4%, 12/29), rifampicin (39.5%, 15/38), tetracycline (38.8%, 19/49), tilmicosin (36.4%, 4/11), clindamycin (30.6%, 15/49), ciprofloxacin (30%, 6/20), erythromycin (22.4%, 11/49), tylosin (18.2%, 2/11), oxacillin (16.3%, 8/49), linezolid (15%, 3/20), teicoplanin (15%, 3/20), fusidic acid (13.1%), imipenem (10.5%, 4/38), vancomycin (7.9%, 3/38), ampicillin (5.5%, 1/18), mupirocin (5.5%, 1/18), fosfomycin (5%, 1/20), and gentamicin (4.1%, 2/49). Twenty-four (49%) S. aureus isolates exhibited multidrug resistance. The investigation highlighted a common occurrence of multidrug-resistant S. aureus strains in the monitored cheese assortments, which can constitute a potential risk for consumers’ health.

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“…Its use appears to be a cost-effective and effective treatment, with no side effects [7]. Staphylococcus aureus is an common bacteria that cause disease in dogs and is thought to be a reservoir for drug-susceptible and drugresistant bacteria such as S. aureus [8,9]. This has led to the development of new alternative treatments, one of which is ethanolic extracts of propolis for topical applications, which have proven to be effective and inexpensive.…”

Section: Introductionmentioning

confidence: 99%

“…All Staphylococcus species that cause infections in humans and domestic animals can develop resistance to antibiotics [8][9][10][11]. The predisposition for staphylococci to develop antimicrobial resistance is a cause of major concern in both human and veterinary medicine [12,13].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Romanian Propolis against Staphylococcus aureus Isolated from Dogs with Superficial Pyoderma: In Vitro Test

Dégi

Herman

Igna

et al. 2022

Veterinary Sciences

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Staphylococcal infection treatment in dogs is frequently associated with adverse side effects, high costs, prolonged treatment, and resistant strain selection. Staphylococcus aureus is the most frequently isolated staphylococci in cases of canine superficial pyoderma. The number of Staphylococcus strains to exhibit primary resistance to various drugs in vitro is increasing. Propolis has a diverse chemical composition and well-known therapeutic properties against bacterial infections. The current investigation evaluated in vitro the antimicrobial activity of the commercial allopathic antimicrobials, Romanian propolis ethanolic extracts, against clinical Staphylococcus aureus strains isolated from superficial dermatitis clinical samples in dogs and two reference strains: Staphylococcus aureus ATCC 25923 and Staphylococcus aureus ATCC 43300, as the MSSA and MRSA positive controls, respectively, in western Romania. We used the microdilution broth technique to evaluate the susceptibility profile of the bacteria. The minimum inhibitory concentration (MIC) of the Romanian propolis ethanolic extract ranged from 6 to 10 μg/mL for all isolates, determined by the broth microdilution method. The MICs of ethanolic Romanian propolis extracts had a pronounced antibacterial activity. These results indicate that propolis can potentially be used and recommended for in vivo experiments as a promising therapeutic agent against Staphylococcus aureus infections in superficial dermatitis of dogs.

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Carriage of Multidrug Resistance Staphylococci in Shelter Dogs in Timisoara, Romania

Cited by 15 publications

References 45 publications

Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus from Hunters and Hunting Dogs

Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus from Hunters and Hunting Dogs

Multidrug Resistant Coagulase-Positive Staphylococcus aureus and Their Enterotoxins Detection in Traditional Cheeses Marketed in Banat Region, Romania

Antibacterial Activity of Romanian Propolis against Staphylococcus aureus Isolated from Dogs with Superficial Pyoderma: In Vitro Test

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