Carrot Molecular Cytogenetics

Iovene, Marina; Grzebelus, Ewa

doi:10.1007/978-3-030-03389-7_8

Cited by 3 publications

(2 citation statements)

References 103 publications

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“…The cluster of rRNA genes was found on chromosome 7, as mentioned above. The distribution of repeats and genes in hogweed is similar to that in the other Apiaceae genomes (Figures S6–S8), including carrot, where different elements of the genome were investigated cytogenetically [for review see (Iovene & Grzebelus, 2019)]. This supports the correspondence of the large scaffolds to chromosomes.…”

Section: Resultssupporting

confidence: 67%

The genome of the toxic invasive species Heracleum sosnowskyi carries an increased number of genes despite absence of recent whole‐genome duplications

Schelkunov,

Shtratnikova,

Klepikova

et al. 2023

The Plant Journal

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SUMMARYHeracleum sosnowskyi, belonging to a group of giant hogweeds, is a plant with large effects on ecosystems and human health. It is an invasive species that contributes to the deterioration of grassland ecosystems. The ability of H. sosnowskyi to produce linear furanocoumarins (FCs), photosensitizing compounds, makes it very dangerous. At the same time, linear FCs are compounds with high pharmaceutical value used in skin disease therapies. Despite this high importance, it has not been the focus of genetic and genomic studies. Here, we report a chromosome‐scale assembly of Sosnowsky's hogweed genome. Genomic analysis revealed an unusually high number of genes (55106) in the hogweed genome, in contrast to the 25–35 thousand found in most plants. However, we did not find any traces of recent whole‐genome duplications not shared with its confamiliar, Daucus carota (carrot), which has approximately thirty thousand genes. The analysis of the genomic proximity of duplicated genes indicates on tandem duplications as a main reason for this increase. We performed a genome‐wide search of the genes of the FC biosynthesis pathway and surveyed their expression in aboveground plant parts. Using a combination of expression data and phylogenetic analysis, we found candidate genes for psoralen synthase and experimentally showed the activity of one of them using a heterologous yeast expression system. These findings expand our knowledge on the evolution of gene space in plants and lay a foundation for further analysis of hogweed as an invasive plant and as a source of FCs.

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Section: Resultssupporting

confidence: 67%

The genome of the toxic invasive species Heracleum sosnowskyi carries an increased number of genes despite absence of recent whole‐genome duplications

Schelkunov,

Shtratnikova,

Klepikova

et al. 2023

The Plant Journal

View full text Add to dashboard Cite

show abstract

“…The cluster of rRNA genes was found on chromosome 7. The distribution of repeats and genes in hogweed is similar to that in the other Apiaceae genomes (Supplementary Figures S6, S7, S8), including carrot, regardless of whether different elements of the genome were investigated cytogenetically (for review see (Iovene and Grzebelus, 2019)). This supports the correspondence of the large scaffolds to chromosomes.…”

Section: Genome Assembly and Annotationsupporting

confidence: 52%

The genome of the toxic invasive speciesHeracleum sosnowskyicarries an increased number of genes despite absence of recent whole-genome duplications

Schelkunov

Shtratnikova

Klepikova

et al. 2023

Preprint

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Heracleum sosnowskyi, belonging to a group of giant hogweeds, is a plant with large effects on ecosystems and human health. It is an invasive species that contributes to the deterioration of grassland ecosystems. The ability of H. sosnowskyi to produce linear furanocoumarins (FCs), photosensitizing compounds, makes it very dangerous. At the same time, linear FCs are compounds with high pharmaceutical value that are used in skin disease therapies. Despite this high importance, it has not been the focus of genetic and genomic studies. Here, we report a chromosome-scale assembly of the Sosnowsky's hogweed genome. Genomic analysis revealed an unusually high number of genes (55 206) in the hogweed genome, in contrast to the 25-35 thousand found in most plants. However, we did not find any traces of recent whole genome duplications not shared with its confamiliar, Daucus carota (carrot), which has approximately thirty thousand genes. The analysis of the genomic proximity of duplicated genes indicates tandem duplications as a main reason for this increase. We performed a genome-wide search of the genes of the FC biosynthesis pathway and their expression in aboveground plant parts. Using a combination of expression data and phylogenetic analysis, we found candidate genes for psoralen synthase and experimentally showed the activity of one of them using a heterologous yeast expression system. These findings expand our knowledge on the evolution of gene space in plants and lay a foundation for further analysis of hogweed as an invasive plant and as a source of FCs.

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Carrot (Daucus carota L.) Breeding

Šimon

2021

Advances in Plant Breeding Strategies: Vegetable Crops

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Carrot Molecular Cytogenetics

Cited by 3 publications

References 103 publications

The genome of the toxic invasive species Heracleum sosnowskyi carries an increased number of genes despite absence of recent whole‐genome duplications

The genome of the toxic invasive species Heracleum sosnowskyi carries an increased number of genes despite absence of recent whole‐genome duplications

The genome of the toxic invasive speciesHeracleum sosnowskyicarries an increased number of genes despite absence of recent whole-genome duplications

Carrot (Daucus carota L.) Breeding

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