Cartilage formation in a hollow fiber bioreactor studied by proton magnetic resonance microscopy

Adipogenesis of Human Adipose-Derived Stem Cells Within Three-Dimensional Hollow Fiber-Based Bioreactors

“…There have been many reports on the use of hollow fiber bioreactor designs used for hepatocyte cultures. 14,[17][18][19][20][21] For example, Martin and Vermette have written a comprehensive review on the role of bioreactors for mass tissue culture.…”

Magnetization Transfer Imaging Provides a Quantitative Measure of Chondrogenic Differentiation and Tissue Development

“…Potter et al, for example, found a good correlation between MRI parameters (e.g., T 1 , T 2 , ADC, and MT) and the biochemical composition of cartilage constructs grown in a bioreactor. 18,31 In addition, Miyata et al showed a strong correlation for T 1 and ADC, but not for T 2 , with the equilibrium Young's modulus of engineered cartilage using articular chondrocytes seeded in an agarose gel scaffold. 32 Similarly, Neves et al found that the effects of perfusion and nutrient diffusion on cell growth and distribution and matrix production in meniscal cartilage constructs could be assessed using MRI and spectroscopy.…”

A flow perfusion bioreactor with controlled mechanical stimulation: Application in cartilage tissue engineering and beyond

“…A cell-suspension medium is passed through either the extra-or intra-capillary space to provide nutrients with cells seeded inside [54,55], or outside of the ibers [52,56]. HFBs have been successfully used [57][58][59][60][61], and in most experiments, 330 μm, 0.2 μm pore, and 150 μm wall thickness polypropylene hollow ibers are arranged along the axis of high-purity glass tubing, and were held in place with silicon rubber [54][55][56][57][58]. Potter and colleagues obtained a cartilaginous construct with one millimeter thickness after four weeks in an HFB [57].…”

“…HFBs have been successfully used [57][58][59][60][61], and in most experiments, 330 μm, 0.2 μm pore, and 150 μm wall thickness polypropylene hollow ibers are arranged along the axis of high-purity glass tubing, and were held in place with silicon rubber [54][55][56][57][58]. Potter and colleagues obtained a cartilaginous construct with one millimeter thickness after four weeks in an HFB [57]. They are used for MSC expansion [62][63][64][65], and Li and colleagues [66,67], found 50-fold expansion within 7 days maintaining self-renewal and multi-lineage differentiation capacity.…”