Cartilage tissue formation through assembly of microgels containing mesenchymal stem cells

Li, Fanyi; Truong, Vinh X.; Fisch, Philipp; Levinson, Clara; Glattauer, Veronica; Zenobi‐Wong, Marcy; Thissen, Helmut; Forsythe, John S.; Frith, Jessica E.

doi:10.1016/j.actbio.2018.07.015

Cited by 117 publications

(150 citation statements)

References 52 publications

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“…Both aggrecan and GAG are molecules that are expressed in the early differentiation process and play a particularly important role in determining the stiffness and tolerance of cartilage tissue, typ-ically articular cartilage tissue 43,44 . In a publication from Li et al (2018) 50 , the accumulation of GAG in human bone marrow derived stem cells (BMSCs) was very high, although the pellet size was larger than those seen in our study. In another study published by Futrega et al (2015) 45 , GAG expression was higher in smaller-sized BMSC pellet (about 200 µm), compared to bigger ones.…”

Section: Discussioncontrasting

confidence: 77%

Method for in vitro production of cartilage microtissues from scaffold-free spheroids composed of human adipose-derived stem cells

et al. 2020

Biomed. Res. Ther.

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Introduction: Cartilage damage is one of the injuries that is difficult for the human body to self-repair due to the avascular and completely mature tissue with only few stem or progenitor cells present. Recently, some studies showed that engineered cartilage tissues could be used to treat or improve this injury. This study aimed to produce the cartilage microtissues from the differentiation of scaffold-free spheroids composed of human adipose-derived stem cells. Methods: Human adipose-derived stem cells (ADSCs) were isolated and expanded following the previously published study. They were then cultured in the non-adherent condition to produce spheroids. The spheroids of the ADSCs were collected and induced into cartilage microtissues in the inducible medium for 21 days. The cartilage microtissue was characterized by some cartilage phenotype markers, including the accumulation of extracellular matrix proteins (aggrecan, glycosaminoglycan, and type II collagen), and the expression of certain genes specific to chondrocytes (Sox9, Col2, Col1, and Acan). Results: The results showed that the expression of chondrocyte-specific genes gradually increased during the 21 days of culture for differentiation. On day 21, the microtissues expressed aggrecan, glycosaminoglycan, and type II collagen proteins. Conclusion: This study demonstrated that cartilage microtissues could easily be produced from scaffold-free spheroids from ADSCs. Thus, cartilage microtissues can be produced in this manner for in vivo transplantation to promote cartilage regeneration, or to produce cartilage tissues for in vitro studies.

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Section: Discussioncontrasting

confidence: 77%

Method for in vitro production of cartilage microtissues from scaffold-free spheroids composed of human adipose-derived stem cells

et al. 2020

Biomed. Res. Ther.

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“…5 wt % in fully swollen stage) and is expected to have minimal toxicity on the encapsulated cells. A seeding density of 2.5 × 10 6 cell mL −1 was used, in accordance with our previous work on 3D cell encapsulation and work reported by other groups . Live/dead staining of the cell‐laden hydrogels showed both cell types are viable with about 85% of live cells (Fig.…”

Section: Introductionsupporting

confidence: 53%

Bioorthogonal hydrogels by thiol–halide click crosslinking with fast gelation time and tunable stability in aqueous media

Truong

Donderwinkel

Frith

2018

J. Polym. Sci. Part A: Polym. Chem.

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“…Frith and coworkers [111] designed tissue-like structures for cartilage tissue formation through bottom-up assembly using cell-laden microgels as the building blocks. Microgels of gelatin norbornene (GelNB) containing human bone marrow derived mesenchymal stem cells (hBMSC) were first produced with a microfluidic device and then cured under visible light (Figure 3a).…”

Section: Engineered Tissues From Controllable Assembly Via Specific Imentioning

confidence: 99%

“…Scheme illustrating microgel formation using a pipette tip-based microfluidic device (a). The 4-arm poly(ethylene glycol)-N-hydroxysuccinimide (PEG-NHS) was used as covalent crosslinker for bottom-up assembly of human bone marrow derived mesenchymal stem cells (hBMSC)-laden microgels for tissue formation (b)[111]. (Reproduced with permission from Elsevier.…”

mentioning

confidence: 99%

Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation

Huang

Abdalla

Xiao

et al. 2020

IJMS

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The concept of three-dimensional (3D) cell culture has been proposed to maintain cellular morphology and function as in vivo. Among different approaches for 3D cell culture, microcarrier technology provides a promising tool for cell adhesion, proliferation, and cellular interactions in 3D space mimicking the in vivo microenvironment. In particular, microcarriers based on biopolymers have been widely investigated because of their superior biocompatibility and biodegradability. Moreover, through bottom-up assembly, microcarriers have opened a bright door for fabricating engineered tissues, which is one of the cutting-edge topics in tissue engineering and regeneration medicine. This review takes an in-depth look into the recent advancements of microcarriers based on biopolymers—especially polysaccharides such as chitosan, chitin, cellulose, hyaluronic acid, alginate, and laminarin—for 3D cell culture and the fabrication of engineered tissues based on them. The current limitations and potential strategies were also discussed to shed some light on future directions.

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Cartilage tissue formation through assembly of microgels containing mesenchymal stem cells

Cited by 117 publications

References 52 publications

Method for in vitro production of cartilage microtissues from scaffold-free spheroids composed of human adipose-derived stem cells

Method for in vitro production of cartilage microtissues from scaffold-free spheroids composed of human adipose-derived stem cells

Bioorthogonal hydrogels by thiol–halide click crosslinking with fast gelation time and tunable stability in aqueous media

Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation

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