CAS agar diffusion assay for the measurement of siderophores in biological fluids

Shin, Sung Heui; Lim, Yong; Lee, Shee Eun; Yang, Nam Woong; Rhee, Joon Haeng

doi:10.1016/s0167-7012(00)00229-3

Cited by 149 publications

(85 citation statements)

References 12 publications

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“…Virulence factors like hemolysin, 2 hemagglutination, 5 serum resistance, 2 gelatinase, 2 cell surface hydrophobicity, 6 and siderophore production 7 were detected and correlated with biofilm production in UPEC isolates.…”

Section: Methodsmentioning

confidence: 99%

Biofilm and multidrug resistance in uropathogenicEscherichia coli

Mittal

Sharma

Chaudhary

2015

Pathogens and Global Health

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Context: Escherichia coli is known as causative agent of urinary tract infections (UTIs) tends to form microcolonies in mucosa lining of urinary bladder known as biofilm. These biofilms make the organism to resist the host immune response, more virulent and lead to the evolution of antibacterial drug resistance by enclosing them in an extracellular biochemical matrix. Aims: This study was done to know the association of various virulence factors and biofilm production in uropathogenic E. coli (UPEC) and antibiotic susceptibility pattern. Settings and design: This study was conducted in Pt. B.D. Sharma PGIMS, Rohtak, Haryana during a period of 1 year from January 2011 to December 2011. Methods and material: Biofilm was detected by microtiter plate (MTP) method, and various virulence factors like hemolysin, hemagglutination, gelatinase, siderophore production, serum resistance, and hydrophobicity were detected. The antibiotic susceptibility testing was done by modified Kirby-Bauer disk diffusion and the disk diffusion method was used to confirm the ESBL, AmpC, MBL production by the UPEC statistical analysis used: The data were analyzed by using SPSS version 17.0. A two-sided P-value of less than or equal to 0.05 was considered to be significant. Results: Biofilm production was found in 18 (13.5%) isolates, more commonly in females (two times). These isolates were found to be resistant to antibiotics common in use and were 100% MDR. Conclusions: Biofilm production makes the organism to be more resistant to antibiotics and virulent as compared to non-biofilm producers.

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Section: Methodsmentioning

confidence: 99%

Biofilm and multidrug resistance in uropathogenicEscherichia coli

Mittal

Sharma

Chaudhary

2015

Pathogens and Global Health

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“…To better characterize the antifungal isolates, their potential production of hydrolytic enzymes and secondary metabolites was studied. Previously described methods were used to assess chitinolytic activity in minimal medium [11], siderophore production [51], amylase activity [14], cellulase activity [16], phosphorus dissolution [25], and nitrogen fixation [22]. Protease activity indicated by casein degradation was determined from distinct zones of clearing in skim milk agar (50 ml sterilized skimmed milk mixed at 55°C with 50 ml of 1/5…”

Section: Elucidation Of Antifungal Spectrum Of the Selected Antagonistsmentioning

confidence: 99%

Biocontrol activities of bacteria from cowdung against the rice sheath blight pathogen

Yang

Zhang²,

YiQing

et al. 2017

J Plant Dis Prot

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To discover bacteria with potential biocontrol activity against the rice sheath blight (RSB) pathogen, Rhizoctonia solani, cowdung (CD) associated bacteria were screened for antifungal activity a dual-culture method. Five potential biocontrol bacteria were identified to species-level based on their colony morphology, physiology, biochemical characteristics, utilization of carbon sources, micromorphology and 16S rRNA sequences. The ability of two selected strains to inhibit RSB was evaluated in vivo and in vitro. The median effective concentration (EC 50 ) of crude extract from Streptomyces cochorusii strain NF0919 (NF0919) culture filtrate was 1.3 lg ml -1 , lower than the EC 50 of Jinggangmycin (a commercial antifungal agent widely used in China). At a concentration of 25.0 lg ml -1 , the crude extract completely inhibited mycelial growth of R. solani. The field biocontrol efficacy after spraying 7 days in 2013 and in 2014 was 78.4 and 98.1% with crude extract from NF0919 culture filtrate and 71.1 and 94.2% with fresh cells of Bacillus amyloliquefaciens strain SB177 (SB177). Results from the 2-year field experiment suggested that the crude extract from NF0919 culture filtrate or fresh cells of SB177 provided better disease control than other fungicides (Jinggangmycin and/ or Kresoxim-methyl). S. cochorusii strain NF0919 and B. amyloliquefaciens strain SB177 have good potential for field application and commercial use against the RSB pathogen.

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“…[6] The chrome aurol sulphonate (CAS) assay detects colour change of CAS-Iron complex from blue to orange after chelation of the bound iron by siderophores. A strong ligand 'L' (e.g., a siderophore) is added to a highly coloured iron dye complex; when the iron ligand complex is formed, the release of the free dye is accompanied by a colour change.…”

Section: Siderophore Production Assaymentioning

confidence: 99%

Haemagglutination and siderophore production as the urovirulence markers of uropathogenic<i> <i>Escherichia</i> coli</i>

Vagarali¹,

Karadesai²,

Patil³

et al. 2008

Indian J Med Microbiol

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A total of 160 strains of Escherichia coli isolated from urine of patients with clinically diagnosed urinary tract infection were included in the study and 50 faecal isolates of E. coli were studied. They were studied for virulence factors, namely mannose-resistant and mannose-sensitive haemagglutination (MRHA, MSHA) and siderophore production.Among 160 urinary isolates of E. coli, 40 (25%) showed MRHA, siderophore production was seen in 156 (97.5%). In 50 faecal isolates, two (4%) were MRHA, four (8%) MSHA and siderophore production in two (4%). The results suggest that MRHA and siderophore production positive strains can be considered as UPEC.

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CAS agar diffusion assay for the measurement of siderophores in biological fluids

Cited by 149 publications

References 12 publications

Biofilm and multidrug resistance in uropathogenicEscherichia coli

Biofilm and multidrug resistance in uropathogenicEscherichia coli

Biocontrol activities of bacteria from cowdung against the rice sheath blight pathogen

Haemagglutination and siderophore production as the urovirulence markers of uropathogenic<i> <i>Escherichia</i> coli</i>

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