Haemagglutination and siderophore production as the urovirulence markers of uropathogenic<i> <i>Escherichia</i> coli</i>

Abstract

A total of 160 strains of Escherichia coli isolated from urine of patients with clinically diagnosed urinary tract infection were included in the study and 50 faecal isolates of E. coli were studied. They were studied for virulence factors, namely mannose-resistant and mannose-sensitive haemagglutination (MRHA, MSHA) and siderophore production.Among 160 urinary isolates of E. coli, 40 (25%) showed MRHA, siderophore production was seen in 156 (97.5%). In 50 faecal isolates, two (4%) were MRHA, four (8%) MSHA and siderophore production in two (4%). The results suggest that MRHA and siderophore production positive strains can be considered as UPEC.

A Comparative Study of Virulence Factors of Escherichia coli Isolated from Urinary Tract Infections with that of Intestinal Escherichia coli Isolated from Apparently Healthy Adults

“…There was statistically significant difference in occurrence of haemagglutination between cases and controls (P<0.01). This finding correlates with previous studies (Vagarali et al, 2008;Kauser et al, 2009). Serum resistance was seen in 55% of the UTI isolates in our study.…”

“…In this study haemagglutination was observed in 61% of cases of UTI which correlates with previous studies (Vagarali et al, 2008;Kauser et al, 2009). MRHA expression was seen in 39% of UTI isolates, the results correlate with Jadhav et al, (2011) (40%) and Siegfred et al, (1994) (43%).…”

Study of uropathogenic Escherichia coli with special reference to its virulence factors

“…1 UTI is the second most common cause of bacterial infection in humans and thus represents a major source of human discomfort. 2 It is now known that there are subgroups of faecal E. coli, that can colonize in the periurethral area, enter urinary tract causing symptomatic disease. It has been known that certain serotypes of E. coli are consistently associated with uropathogenicity and are designated as uropathogenic E. coli that expresses chromosomally encoded virulence markers.…”

“…It has been known that certain serotypes of E. coli are consistently associated with uropathogenicity and are designated as uropathogenic E. coli that expresses chromosomally encoded virulence markers. 2 For the first time in the late 1970s it was recognized that E. coli strains causing urinary tract infections typically agglutinate human erythrocytes despite the presence of mannose and this was mediated mainly by fimbriae. 3 The important virulence factors in the pathogenesis of urinary tract infection (UTI) include adhesions (P fimbriae, certain other mannose-resistant adhesins, and type 1 fimbriae), the aerobactin system, hemolysin, K capsule, and resistance to serum killing, hemolysin production and siderophore production.…”

New Natural Medium Using Vitis vinirfera for Siderophore Production from Clinical Isolates of Klebsiella pneumonia

“…Thus, iron is a key element of bacterial pathogenesis [19,20]. Vagrali et al, [21] carried out studies that showed siderophores considered as urovirulence markers of uropathogenic E. coli, so siderophore production may be a necessary feature of a virulent bacterium but not a determinant of virulence. Staphylococcus aureus, and Klebsiella pneumoniae produce siderophores and study it as a virulence factor, the results showed ability of all strains to produce siderophores, which confirmed its roles in pathogenesis [17].…”