CASC11 promotes aggressiveness of prostate cancer cells through miR-145/IGF1R axis

Capik, Ozel; Şanli, Fatma; Kurt, Ali; Suer, İlknur; Kaya, Murat; Ittmann, Michael; Karataş, Ömer Faruk

doi:10.1038/s41391-021-00353-0

Cited by 20 publications

(7 citation statements)

References 52 publications

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“…Although most of these lncRNAs are not conserved in zebrafish, we were able to identify the homologous genes of 6 lncRNAs ( Table S1 ). Among the differentially expressed genes, multiple lncRNAs were previously found to regulate cell proliferation and migration, including VIM-AS1 ( 12 , 13 ), CASC11 ( 14 , 15 ), DGUOK-AS1 ( 16 , 17 ), SNHG11 ( 18 , 19 ), and SMAD5-AS1 ( 20 ).…”

Section: Resultsmentioning

confidence: 99%

Single-cell profiling and functional screening reveal crucial roles for lncRNAs in the epidermal re-epithelialization of human acute wounds

Xiao,

Zhang,

Liu

et al. 2024

Front. Surg.

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ObjectivesRe-epithelialization is an important physiological process for repairing skin barrier function during wound healing. It is primarily mediated by coordinated migration, proliferation, and differentiation of keratinocytes. Long noncoding RNAs (lncRNAs) are essential components of the noncoding genome and participate in various biological processes; however, their expression profiles and function in re-epithelialization during wound healing have not been established.MethodsWe investigated the distribution of lncRNAs during wound re-epithelialization by comparing the genomic profiles of uninjured skin and acute wound (AW) from healthy donors. We performed functional screening of differentially expressed lncRNAs to identify the important lncRNAs for re-epithelialization.ResultsThe expression of multiple lncRNAs is changed during human wound re-epithelialization process. We identified VIM-AS1, SMAD5-AS1, and LINC02581 as critical regulators involved in keratinocyte migration, proliferation, and differentiation, respectively.ConclusionLncRNAs play crucial regulatory roles in wound re-epithelialization. We established lncRNA expression profile in human acute wounds compared with intact skin, offering valuable insights into the physiological mechanisms underlying wound healing and potential therapeutic targets.

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Section: Resultsmentioning

confidence: 99%

Single-cell profiling and functional screening reveal crucial roles for lncRNAs in the epidermal re-epithelialization of human acute wounds

Xiao,

Zhang,

Liu

et al. 2024

Front. Surg.

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“…Then, 1 μg total RNA from each sample was reversed transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystem™, Thermo Fisher Scientific). qRT-PCR experiments were carried out on a Rotor-Gene qRT-PCR System (Qiagen) as described previously (Capik et al, 2021). All qRT-PCR reactions were performed in triplicates for each primer pair, which are listed in Supporting Information: Table 1.…”

Section: Rna Isolation Cdna Synthesis and Qrt-pcrmentioning

confidence: 99%

Overexpression of POFUT1 promotes malignant phenotype and mediates perineural invasion in head and neck squamous cell carcinoma

Barlak,

Kusdemir,

Gumus

et al. 2023

Cell Biology International

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Head and neck squamous cell carcinoma (HNSCC) is one of the most aggressive neoplasms, which requires more effective prevention and treatment modalities. Previous studies found that protein O‐fucosyltransferase 1 (POFUT1) upregulation promotes carcinogenesis, although the potential roles, underlying molecular mechanisms, and biological implications of POFUT1 in HNSCC were not investigated. In this study, in silico analyses referred POFUT1 as a potential oncogene in HNSCC. Further analysis of tumor and normal tissue samples as well as HNSCC cells with quantitative real‐time polymerase chain reaction, Western blot analysis, and immunohistochemistry showed significant overexpression of POFUT1 in HNSCC clinical tumor tissue specimens and cell lines compared to corresponding controls. In vitro investigations revealed that overexpression of POFUT1 promoted phenotypes associated with cancer aggressiveness and its knockdown in HNSCC cells suppressed those phenotypes. Further xenograft experiments demonstrated that POFUT1 is an oncogene in vivo for HNSCC. Immunohistochemical analysis with human clinical samples and cancer cell‐dorsal root ganglion ex‐vivo coculture model showed that deregulation of POFUT1 is involved in the perineural invasion of HNSCC cells. These results suggest POFUT1 expression as a potential prognostic marker for patients with head and neck cancer and highlight its potential as a target for HNSCC therapy, although more molecular clues are needed to better define the functions of POFUT1 related to HNSCC carcinogenesis.

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“…The EPIN of CASC11 promoter is also affected by variant rs10090154, the same well known variant associated with risk of developing prostate carcinoma that we introduced with MYC EPIN 42,43 (Supplementary Data 10). Interestingly, CASC11 is known to enhance prostate cancer aggressiveness and is regulated by C-MYC 44 , while being close to the MYC gene on chromosome 8. The promoter binds 6 proteins: TFAP2C, SP3, SP1, PKNOX1, NR2C2 and KLF5.…”

Section: Examples: Snps Path Analysis Of Myc Casc11 and Gata2 Promotersmentioning

confidence: 99%

The PENGUIN approach to reconstruct protein interactions at enhancer-promoter regions and its application to prostate cancer

Armaos,

Serra,

Núñez-Carpintero

et al. 2023

Nat Commun

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We introduce Promoter-Enhancer-Guided Interaction Networks (PENGUIN), a method for studying protein-protein interaction (PPI) networks within enhancer-promoter interactions. PENGUIN integrates H3K27ac-HiChIP data with tissue-specific PPIs to define enhancer-promoter PPI networks (EPINs). We validated PENGUIN using cancer (LNCaP) and benign (LHSAR) prostate cell lines. Our analysis detected EPIN clusters enriched with the architectural protein CTCF, a regulator of enhancer-promoter interactions. CTCF presence was coupled with the prevalence of prostate cancer (PrCa) single nucleotide polymorphisms (SNPs) within the same EPIN clusters, suggesting functional implications in PrCa. Within the EPINs displaying enrichments in both CTCF and PrCa SNPs, we also show enrichment in oncogenes. We substantiated our identified SNPs through CRISPR/Cas9 knockout and RNAi screens experiments. Here we show that PENGUIN provides insights into the intricate interplay between enhancer-promoter interactions and PPI networks, which are crucial for identifying key genes and potential intervention targets. A dedicated server is available at https://penguin.life.bsc.es/.

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CASC11 promotes aggressiveness of prostate cancer cells through miR-145/IGF1R axis

Cited by 20 publications

References 52 publications

Single-cell profiling and functional screening reveal crucial roles for lncRNAs in the epidermal re-epithelialization of human acute wounds

Single-cell profiling and functional screening reveal crucial roles for lncRNAs in the epidermal re-epithelialization of human acute wounds

Overexpression of POFUT1 promotes malignant phenotype and mediates perineural invasion in head and neck squamous cell carcinoma

The PENGUIN approach to reconstruct protein interactions at enhancer-promoter regions and its application to prostate cancer

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