Case-control study of ADARB1 and ADARB2 gene variants in migraine

Abstract

BackgroundMigraine causes crippling attacks of severe head pain along with associated nausea, vomiting, photophobia and/or phonophobia. The aim of this study was to investigate single nucleotide polymorphisms (SNPs) in the adenosine deaminase, RNA-specific, B1 (ADARB1) and adenosine deaminase, RNA specific, B2 (ADARB2) genes in an Australian case–control Caucasian population for association with migraine. Both candidate genes are highly expressed in the central nervous system and fit criteria for migraine neuropathology. SNPs in the ADARB2 gene were previously found to be positively associated with migraine in a pedigree-based genome wide association study using the genetic isolate of Norfolk Island, Australia. The ADARB1 gene was also chosen for investigation due to its important function in editing neurotransmitter receptor transcripts.MethodsFour SNPs in ADARB1 and nine in ADARB2 were selected by inspecting blocks of linkage disequilibrium in Haploview for genotyping using either TaqMan or Sequenom assays. These SNPs were genotyped in two-hundred and ninety one patients who satisfied the International Classification of Headache Disorders-II 2004 diagnostic criteria for migraine, and three-hundred and fourteen controls, and PLINK was used for association testing.ResultsChi-square analysis found no significant association between any of the SNPs tested in the ADARB1 and ADARB2 genes in this study and the occurrence of migraine.ConclusionsIn contrast to findings that SNPs in the ADARB2 gene were positively associated with migraine in the Norfolk Island population, we find no evidence to support the involvement of RNA editing genes in migraine susceptibility in an Australian Caucasian population.

Genetic overlap between temporomandibular disorders and primary headaches: A systematic review

“… - COMT genotype does not impact migraine susceptibility or phenotype. Gasparini, C. F., et al (2015) [67] Case-control study MO (64); MA (227); & controls (314) ADARB1 - no significant association between any of the SNPs tested in the ADARB1 and ADARB2 genes in this study and the development of migraine. - no evidence to support the involvement of RNA editing genes in migraine susceptibility in an Australian Caucasian population.…”

“…From the included studies, a brief description of the study design and sample is provided, followed by identifying the genes involved in the pathophysiology of either PHD ( Table 3 ) [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] , [66] , [67] , [68] , [69] , [70] , [71] , [72] , [73] , [74] , [75] , [76] , [77] , [78] , [79] , [80] or TMD ( Table 4 ) [81] , [82] , [83] , [84] , [85] , [86] , [87] , [88] , [89] , [90] , [91] , [92] , [93] , [94] ,…”

Genome-wide association study reveals a locus in ADARB2 for complete freedom from headache in Danish Blood Donors

“…However, the variants in ADARB2 found in the island population were located more than 400,000 bp upstream of our lead SNP. Furthermore, the association between ADARB2 and migraine was not replicated in a hypothesis-driven case-control study or a large GWAS meta-analysis of more than 100,000 migraine cases 31 , 32 . The lead SNP (rs7904615) from our analysis was not associated with migraine ( p = 0.85), cluster headache ( p = 0.12) or tension-type headache ( p = 0.39) in the respective GWAS summary statistics (unpublished tension-type GWAS summary statistics provided by the Norwegian HUNT study) 32 – 34 .…”