Case report on pulmonary disease due to coinfection of Mycobacterium tuberculosis and Mycobacterium abscessus : Difficulty in diagnosis

Ishiekwene, Celestine; Subran, Mala; Ghitan, Monica; Kuhn-Basti, Margaret; Chapnick, Edward K.; Lin, Yu Shia

doi:10.1016/j.rmcr.2017.01.011

Cited by 8 publications

(7 citation statements)

References 6 publications

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“…20,21 Our study also showed co-infection with MTB and NTM in 14 women. Moreover, in nine of these women, NTM were positive both in the endometrium and in the POD aspirate.…”

Section: Main Findingssupporting

confidence: 63%

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Significance of isolating non‐tuberculous mycobacterial organisms in infertile women with tubal disease: an observational study

Prabhu

Pandiyan

Sujatha

et al. 2019

BJOG

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Objectives To explore whether non-tuberculous mycobacteria (NTM) are associated with tubal disease leading to infertility. Design Prospective observational study.Setting Teaching hospital.Population Women with tubal factor infertility.Methods In all, 173 infertile women with tubal disease were investigated for genital tuberculosis, Chlamydia trachomatis and Neisseria gonorrhoeae using polymerase chain reaction, culture and histopathological examination. On culture, NTM were grown in 23.7% of endometrial samples. The mycolic characteristics of these organisms were analysed.Main outcome measure Whether NTM are associated with tubal disease leading to infertility. ResultsThe organisms identified in association with tubal disease were Mycobacterium tuberculosis in 30%, gonococci in 1.7%, Chlamydia in 7.5% and NTM in 23.7% of cases.Mycobacterium chelonae was the predominant organism identified by high-performance liquid chromatography. In ten women, for whom there was laparoscopic evidence of tubal disease, the only organism that was grown was NTM, and the tests for other organisms were negative. Tests for possible environment (theatre, instruments) contamination was reported negative.Conclusion While evaluating infertile women for tubal disease, culture studies revealed a high prevalence of NTM in the endometrium. In the absence of M. tuberculosis, gonococci and Chlamydia infection, the presence of NTM suggests the possibility that these organisms may be responsible for tubal damage leading to infertility.

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“…20,21 Our study also showed co-infection with MTB and NTM in 14 women. Moreover, in nine of these women, NTM were positive both in the endometrium and in the POD aspirate.…”

Section: Main Findingssupporting

confidence: 63%

“…There have been reports on concomitant infection with MTB and NTM in pulmonary disease. 20,21 Our study also showed co-infection with MTB and NTM in 14 women. In the study by Jain et al 22 one of the risk factors found to be associated with NTM infection was a past or present history of tuberculosis.…”

Section: Main Findingssupporting

confidence: 63%

Significance of isolating non‐tuberculous mycobacterial organisms in infertile women with tubal disease: an observational study

Prabhu

Pandiyan

Sujatha

et al. 2019

BJOG

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“…Additionally, patients suffering from cystic fibrosis or other immunosuppressive condition can be infected by different mycobacteria at the same time. In fact, co-infections with M. abscessus and M. tuberculosis have been reported [27, 28]. The strong bactericidal effect of FP-11g against M. smegmatis supports investigation of this compound and its derivatives for its potential use as a broad antimycobacterial agent.…”

Section: Discussionmentioning

confidence: 95%

Mechanism and resistance for antimycobacterial activity of a fluoroquinophenoxazine compound

Garcia

Annamalai

Wang

et al. 2018

Preprint

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24We have previously reported the inhibition of bacterial topoisomerase I activity by a 25 fluoroquinophenoxazine compound (FP-11g) with a 6-bipiperidinyl lipophilic side chain that 26 exhibited promising antituberculosis activity (MIC = 2.5 µM against Mycobacterium 27 tuberculosis, SI = 9.8). Here, we found that the compound is bactericidal towards 28Mycobacterium smegmatis, resulting in greater than 5 Log10 reduction in colony-forming units 29[cfu]/mL following a 10 h incubation at 1.25 µM (4X MIC) concentration. Growth inhibition 30 (MIC = 50 µM) and reduction in cfu could also be observed against a clinical isolate of 31 Mycobacterium abscessus. Stepwise isolation of resistant mutants of M. smegmatis was 32 conducted to explore the mechanism of resistance. Mutations in the resistant isolates were 33 identified by direct comparison of whole-genome sequencing data from mutant and wild-type 34 isolates. These include mutations in genes likely to affect the entry and retention of the 35 compound. FP-11g inhibits Mtb topoisomerase I and Mtb gyrase with IC50 of 0.24 and 31.5 µM, 36 respectively. Biophysical analysis showed that FP-11g binds DNA as an intercalator but the IC50 37 for inhibition of Mtb topoisomerase I activity is >10 fold lower than the compound 38 concentrations required for producing negatively supercoiled DNA during ligation of nicked 39 circular DNA. Thus, the DNA-binding property of FP-11g may contribute to its 40 antimycobacterial mechanism, but that alone cannot account for the observed inhibition of Mtb 41 topoisomerase I. 42 43 157 Enzyme assays 158

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“…abscessus and M . tuberculosis have been reported [34, 35]. The strong bactericidal effect of FP-11g against M .…”

Section: Discussionmentioning

confidence: 99%

Mechanism and resistance for antimycobacterial activity of a fluoroquinophenoxazine compound

et al. 2019

View full text Add to dashboard Cite

We have previously reported the inhibition of bacterial topoisomerase I activity by a fluoroquinophenoxazine compound (FP-11g) with a 6-bipiperidinyl lipophilic side chain that exhibited promising antituberculosis activity (MIC = 2.5 μM against Mycobacterium tuberculosis , SI = 9.8). Here, we found that the compound is bactericidal towards Mycobacterium smegmatis , resulting in greater than 5 Log 10 reduction in colony-forming units [cfu]/mL following a 10 h incubation at 1.25 μM (4X MIC) concentration. Growth inhibition (MIC = 50 μM) and reduction in cfu could also be observed against a clinical isolate of Mycobacterium abscessus . Stepwise isolation of resistant mutants of M . smegmatis was conducted to explore the mechanism of resistance. Mutations in the resistant isolates were identified by direct comparison of whole-genome sequencing data from mutant and wild-type isolates. These include mutations in genes likely to affect the entry and retention of the compound. FP-11g inhibits Mtb topoisomerase I and Mtb gyrase with IC 50 of 0.24 and 27 μM, respectively. Biophysical analysis showed that FP-11g binds DNA as an intercalator but the IC 50 for inhibition of Mtb topoisomerase I activity is >10 fold lower than the compound concentrations required for producing negatively supercoiled DNA during ligation of nicked circular DNA. Thus, the DNA-binding property of FP-11g may contribute to its antimycobacterial mechanism, but that alone cannot account for the observed inhibition of M tb topoisomerase I.

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Case report on pulmonary disease due to coinfection of Mycobacterium tuberculosis and Mycobacterium abscessus : Difficulty in diagnosis

Cited by 8 publications

References 6 publications

Significance of isolating non‐tuberculous mycobacterial organisms in infertile women with tubal disease: an observational study

Significance of isolating non‐tuberculous mycobacterial organisms in infertile women with tubal disease: an observational study

Mechanism and resistance for antimycobacterial activity of a fluoroquinophenoxazine compound

Mechanism and resistance for antimycobacterial activity of a fluoroquinophenoxazine compound

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