Caspase-3/MAPK pathways as main regulators of the apoptotic effect of the phyto-mediated synthesized silver nanoparticle from dried stem of Eleutherococcus senticosus in human cancer cells

Kim, Chun-Gon; Castro-Aceituno, Verónica; Abbai, Ragavendran; Lee, Hyun A.; Simu, Shakina Yesmin; Han, Yaxi; Hurh, Joon; Kim, Yeon-Ju; Yang, Deok Chun

doi:10.1016/j.biopha.2018.01.050

Cited by 61 publications

(29 citation statements)

References 43 publications

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“…Therefore, augmentation of cytotoxicity by formulating ICA into phytosomes is further supported by these data. Enhancement of caspase-3 by nanostructured formulations has been previously reported [37,57,58]. In addition, caspase-3 results are consistent in that the observed MMP disturbances that initiate caspase-3 activation promote typical apoptotic features.…”

Section: Discussionsupporting

confidence: 86%

RETRACTED: Optimized Icariin Phytosomes Exhibit Enhanced Cytotoxicity and Apoptosis-Inducing Activities in Ovarian Cancer Cells

et al. 2020

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Icariin (ICA) is a flavonol glycoside that has pleiotropic pharmacological actions. It has cytotoxic effects against ovarian cancer cells and increases their chemosensitivity to chemotherapeutic drugs. Phytosomes are identified for their potential in drug delivery of cytotoxic agents. Thus, the purpose of this study was to determine the potential enhancement of ICA cytotoxicity activity in OVCAR-3 ovarian cancer cells via its formulation in phytosomes. ICA-phytosomal formulation was optimized using a Box–Behnken design. Particle size, shape, and in vitro drug release were used to characterize the optimized formula. The optimized formulation exhibited enhanced in vitro drug release. ICA-phytosomes exhibited enhanced cytotoxicity against ovarian cancer cells. Cell cycle analysis indicated accumulation of cells challenged with ICA-phytosomes in G2/M and pre-G1 phases. Staining of cells with annexin V indicated significant elevation of percentage cells with early and late apoptosis as well as total cell death. In addition, the formulation significantly disturbed mitochondrial membrane potential and cellular content of caspase 3. In addition, intracellular release of reactive oxygen species (ROS) was enhanced by ICA-phytosomes. In conclusion, phytosome formulation of ICA significantly potentiates its cytotoxic activities against OVCAR-3 cells. This is mediated, at least partly, by enhanced ICA cellular permeation, apoptosis, and ROS.

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Section: Discussionsupporting

confidence: 86%

RETRACTED: Optimized Icariin Phytosomes Exhibit Enhanced Cytotoxicity and Apoptosis-Inducing Activities in Ovarian Cancer Cells

et al. 2020

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“…Thirdly, the interaction of biological NPs to cell membranes makes changes in the cell permeability and mitochondrial dysfunction [ 142 , 143 ]. Kim et al [ 144 ] demonstrated that the activation of p38 MAPK and Caspase-3 at gene and protein expression levels results in response to nanoparticles. The general mechanism for anticancer activity biological NPs concluded from various studies is given in Fig.…”

Section: Main Textmentioning

confidence: 99%

Biogenic nanoparticles: a comprehensive perspective in synthesis, characterization, application and its challenges

Patil

Chandrasekaran

2020

Journal of Genetic Engineering and Biotechnology

214

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Background Translating the conventional scientific concepts into a new robust invention is a much needed one at a present scenario to develop some novel materials with intriguing properties. Particles in nanoscale exhibit superior activity than their bulk counterpart. This unique feature is intensively utilized in physical, chemical, and biological sectors. Each metal is holding unique optical properties that can be utilized to synthesize metallic nanoparticles. At present, versatile nanoparticles were synthesized through chemical and biological methods. Main body of abstract Metallic nanoparticles pose numerous scientific merits and have promising industrial applications. But concerning the pros and cons of metallic nanoparticle synthesis methods, researchers elevate to drive the synthesis process of nanoparticles through the utilization of plant resources as a substitute for use of chemicals and reagents under the theme of green chemistry. These synthesized nanoparticles exhibit superior antimicrobial, anticancer, larvicidal, leishmaniasis, wound healing, antioxidant, and as a sensor. Therefore, the utilization of such conceptualized nanoparticles in treating infectious and environmental applications is a warranted one. Conclusion Green chemistry is a keen prudence method, in which bioresources is used as a template for the synthesis of nanoparticles. Therefore, in this review, we exclusively update the context of plant-based metallic nanoparticle synthesis, characterization, and applications in detailed coverage. Hopefully, our review will be modernizing the recent trends going on in metallic nanoparticles synthesis for the blooming research fraternities. Graphical abstract

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“…Apoptosis is also known as programmed cell death (PCD). The cell apoptosis is characterized by caspase‐dependent, cell contraction and apoptotic body formation . SH‐SY5Y cells were stained with Hoechst 33258, and then, we need to use under an inverted fluorescence microscope to observe them in this experiment.…”

Section: Discussionmentioning

confidence: 99%

“…The cell apoptosis is characterized by caspase-dependent, cell contraction and apoptotic body formation. [35] SH-SY5Y cells were stained with Hoechst 33258, and then, we need to use under an inverted fluorescence microscope to observe them in this experiment. We found that they were handled with Meriolin1 for 48 h, the chromatin of the cells became shrunk, and apoptotic bodies appeared.…”

Section: Discussionmentioning

confidence: 99%

Meriolin1 induces cell cycle arrest, apoptosis, autophagy and targeting the Akt/MAPKs pathways in human neuroblastoma SH-SY5Y cells

Wang

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Meriolins, a kind of chemical hybrid between meridianins and variolins, have lately been determined as kinase inhibitors and reportedly have antitumour activity. However, there is currently no in‐depth study for the action mechanism. This study aimed to elucidate the potentially antitumour action mechanism of Meriolin1 on human neuroblastoma (SH‐SY5Y) cells. Methods Firstly, cell viability was detected by MTT assay. Secondly, cell cycle, cell apoptosis, cell autophagy, reactive oxygen species and mitochondrial membrane potential (ΔΨm) were measured by flow cytometry. Then, cell cycle‐associated proteins, Bcl‐2 family proteins, Akt/MAPK proteins and autophagy‐associated proteins expressions were evaluated by Western blot. Bcl‐2 and Bax mRNA expressions were also evaluated by qRT‐PCR. Furthermore, cell adhesion assay and Hoechst 33258 fluorescent staining were carried out to detect the effect of Meriolin1 on cell adhesion and morphology. Finally, to gain further insight into mechanism of action of Meriolin1 to CDK protein, the molecular docking study was performed by using the CDOCKER module of DS software. Key findings Meriolin1 could exert the antitumour activity on SH‐SY5Y cells by inducing cell cycle arrest, cell autophagy, the mitochondrion‐dependent cell apoptosis and targeting the Akt/MAPKs signalling pathway. Conclusions Meriolin1 might be a promising therapeutic candidate for neuroblastoma.

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Caspase-3/MAPK pathways as main regulators of the apoptotic effect of the phyto-mediated synthesized silver nanoparticle from dried stem of Eleutherococcus senticosus in human cancer cells

Cited by 61 publications

References 43 publications

RETRACTED: Optimized Icariin Phytosomes Exhibit Enhanced Cytotoxicity and Apoptosis-Inducing Activities in Ovarian Cancer Cells

RETRACTED: Optimized Icariin Phytosomes Exhibit Enhanced Cytotoxicity and Apoptosis-Inducing Activities in Ovarian Cancer Cells

Biogenic nanoparticles: a comprehensive perspective in synthesis, characterization, application and its challenges

Meriolin1 induces cell cycle arrest, apoptosis, autophagy and targeting the Akt/MAPKs pathways in human neuroblastoma SH-SY5Y cells

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