Caspases: evolutionary aspects of their functions in vertebrates

Sakamaki, Kazuhiro; Satou, Yutaka

doi:10.1111/j.1095-8649.2009.02184.x

Cited by 111 publications

(106 citation statements)

References 131 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…For example, humans, but not mice, encode caspase-10 [36]. It was worth investigating whether murine monocyte activation may involve the activity of other caspases.…”

Section: Resultsmentioning

confidence: 99%

Caspase-8 inhibition represses initial human monocyte activation in septic shock model

et al. 2016

View full text Add to dashboard Cite

In septic patients, the onset of septic shock occurs due to the over-activation of monocytes. We tested the therapeutic potential of directly targeting innate immune cell activation to limit the cytokine storm and downstream phases. We initially investigated whether caspase-8 could be an appropriate target given it has recently been shown to be involved in microglial activation. We found that LPS caused a mild increase in caspase-8 activity and that the caspase-8 inhibitor IETD-fmk partially decreased monocyte activation. Furthermore, caspase-8 inhibition induced necroptotic cell death of activated monocytes. Despite inducing necroptosis, caspase-8 inhibition reduced LPS-induced expression and release of IL-1β and IL-10. Thus, blocking monocyte activation has positive effects on both the pro and anti-inflammatory phases of septic shock. We also found that in primary mouse monocytes, caspase-8 inhibition did not reduce LPS-induced activation or induce necroptosis. On the other hand, broad caspase inhibitors, which have already been shown to improve survival in mouse models of sepsis, achieved both. Thus, given that monocyte activation can be regulated in humans via the inhibition of a single caspase, we propose that the therapeutic use of caspase-8 inhibitors could represent a more selective alternative that blocks both phases of septic shock at the source.

show abstract

“…For example, humans, but not mice, encode caspase-10 [36]. It was worth investigating whether murine monocyte activation may involve the activity of other caspases.…”

Section: Resultsmentioning

confidence: 99%

Caspase-8 inhibition represses initial human monocyte activation in septic shock model

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Caspases are a family of endoproteases, activated in response to diverse cell death stimuli and providing critical links in cell regulatory networks controlling inflammation and cell death. It is now well established that certain caspases (caspase-8, caspase-9, and caspase-10 in humans) play upstream "initiator" roles in apoptosis by coupling cell death stimuli to the downstream "effector" caspases (caspase-3, caspase-6, and caspase-7) (Sakamaki and Satou, 2009). The initiator caspases appear to be highly specific proteases that cleave few proteins other than their own precursors and the downstream effector caspases (Stennicke et al, 1998;Slee et al, 1999;Cullen and Martin, 2009).…”

Section: Discussionmentioning

confidence: 99%

Diatom-derived oxylipins induce cell death in sea urchin embryos activating caspase-8 and caspase 3/7

et al. 2016

View full text Add to dashboard Cite

a b s t r a c tDiatoms are an important class of unicellular algae that produce bioactive secondary metabolites with cytotoxic activity collectively termed oxylipins, including polyunsaturated aldehydes (PUAs), hydroxyacids (HEPEs), oxo-acids and epoxyalcohols. Previous results showed that at higher concentrations, the PUA decadienal induced apoptosis on copepods and sea urchin embryos via caspase-3 activation; at lower concentrations decadienal affected the expression levels of the caspase-8 gene in embryos of the sea urchin Paracentrotus lividus. In the present work, we studied the effects of other common oxylipins produced by diatoms: two PUAs (heptadienal and octadienal) and four hydroxyacids (5-, 9-11-and 15-HEPE) on P. lividus cell death and caspase activities. Our results showed that (i) at higher concentrations PUAs and HEPEs induced apoptosis in sea urchin embryos, detected by microscopic observation and through the activation of caspase-3/7 and caspase-8 measured by luminescent assays; (ii) at low concentrations, PUAs and HEPEs affected the expression levels of caspase-8 and caspase-3/7 (isolated for the first time here in P. lividus) genes, detected by Real Time qPCR. These findings have interesting implications from the ecological point of view, given the importance of diatom blooms in nutrient-rich aquatic environments.

show abstract

“…5), which groups the sea bass caspase-8 with the ones of other fish, with closer relation with other Percomorpha fish. Of notice is the apparent common origin of the fish caspase-8, with that of non-fish caspase-8, CARD-caspase-8 and caspase-10 (reviewed in [23]). …”

Section: Speciesmentioning

confidence: 98%

Molecular cloning of sea bass (Dicentrarchus labrax L.) caspase-8 gene and its involvement in Photobacterium damselae ssp. piscicida triggered apoptosis

Reis

Costa-Ramos²,

Vale³

et al. 2010

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Caspases: evolutionary aspects of their functions in vertebrates

Cited by 111 publications

References 131 publications

Caspase-8 inhibition represses initial human monocyte activation in septic shock model

Caspase-8 inhibition represses initial human monocyte activation in septic shock model

Diatom-derived oxylipins induce cell death in sea urchin embryos activating caspase-8 and caspase 3/7

Molecular cloning of sea bass (Dicentrarchus labrax L.) caspase-8 gene and its involvement in Photobacterium damselae ssp. piscicida triggered apoptosis

Contact Info

Product

Resources

About