Parkia javanica is a well-known ethno-botanical plant of the north-east region of India. Ethnic communities of the region use several parts, including fruits, of this plant for the treatment of various ailments like diarrhoea, dysentery, cholera, food poisoning etc. In addition fruits are consumed by the local tribes of the north-east as food supplements. With this background we have performed chemical characterisation, and investigated the antimicrobial and antibiofilm potentiality of ethyl acetate fraction of Parkia javanica fruit extract (PJE) against model biofilm-causing microorganism Pseudomonas aeruginosa. PJE was initially prepared from fruit extract, and assayed by IR and UV spectroscopy and HPLC to confirm the presence of compounds. HPLC and NMR analysis reveals that PJE contains flavone compounds baicalein, quercetin and chrysin. PJE, baicalein, quercetin and chrysin were then tested for antimicrobial and antibiofilm activity against P. aeruginosa. PJE showed very significant antimicrobial activity against P. aeruginosa wherein the minimum inhibitory concentration was found at 180 mg mL
À1. Interestingly, the antibiofilm study illustrates that minimum concentration of PJE (30 mg mL À1 ) exhibited maximum activity whereas maximum concentration of PJE (90 mg mL À1 ) exhibited minimum antibiofilm activity. It was also observed that compounds baicalein, quercetin and chrysin separately show lower to moderate antibiofilm activity in comparison to PJE. Molecular docking study indicates that baicalein, quercetin and chrysin have good binding affinity with bacterial quorum sensing and motility associated proteins. Furthermore, we have also observed that in comparison with higher concentration, lower concentration of PJE exhibited better attenuation in swarming motility, secretion of proteases and virulence factors like pyoverdin and pyocyanin. AFM study reveals that aggregates in PJE are smaller in size at low concentrations than at higher concentrations. Observations in the present study suggest that PJE as a whole shows higher antibiofilm activity at low concentration whereas individual compounds have comparatively lower antibiofilm activity. This validates the phytomedicinal significance of Parkia javanica against bacterial biofilms.