Casticin inhibits PDGF-induced proliferation and migration of airway smooth muscle cells

Dai, Ying; Cheng, Ran; Gao, Jie; Liu, Ying; Lou, Chunyan; Li, Yanyang

doi:10.1016/j.ejphar.2018.04.016

Cited by 11 publications

(9 citation statements)

References 27 publications

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“…Likewise, peaks 22 and 23 were identified as dimethyl myricetin derivatives one of them probably syringetin [46] (demethylated molecules at 330.03610, (demethylated molecule) 315.01309 (didemethylated molecule). Peak 26 was determined as a polymethoxylated flavonol possibly jaceidin (C 18 H 15 O 8 , CAS 19536-25-5, PubChem CID 5464461) [47] which has demonstrated protective activity against chromosomal damage in mitogen induced human lymphocytes [48] and peak 30 as a polymethoxylated flavonol possibly, the methyl jaceidin derivative casticin, [49] this compound is very bioactive compound and potent anti-inflammatory agent [50,51,52].…”

Section: Resultsmentioning

confidence: 99%

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

et al. 2019

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The decoction of the local plant Baccharis grisebachii is used as a digestive, gastroprotective, external cicatrizing agent and antiseptic in Argentine. A lyophilized decoction (BLD) from the aerial parts of this plant was evaluated regarding its anti-ulcer, antioxidant and cytotoxic activities and the bioactivities were supported by UHPLC-MS metabolome fingerprinting which revealed the presence of several small bioactive compounds. The antioxidant properties were evaluated by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The BLD showed a moderate free radical scavenging activity in the DPPH (EC50 = 106 µg/mL) and lipid peroxidation in erythrocytes assays (67%, at 250 µg/mL). However, the BLD had the highest gastroprotective effect at a dose of 750 mg/kg with a ninety-three percent inhibition of damage through a mechanism that involve NO and prostaglandins using the ethanol-induced gastric damage in a standard rat model. On the other hand, BLD does not induce cytotoxic changes on human tumor and no-tumor cell lines at the concentrations assayed. Regarding the metabolomic analysis, thirty-one compounds were detected and 30 identified based on UHPLC-OT-MS including twelve flavonoids, eleven cinnamic acid derivatives, one coumarin, one stilbene and two other different phenolic compounds. The results support that the medicinal decoction of Baccharis grisebachii is a valuable natural product with gastroprotective effects and with potential to improve human health that opens a pathway for the development of important phytomedicine products.

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Section: Resultsmentioning

confidence: 99%

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

et al. 2019

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“… 25 27 PDGF has also been reported to induce the proliferation, synthesis, and migration of ASMCs, as well as the production of inflammatory factors. 23 28 29 30 In our study, PDGF remarkably increased the proliferation, migration, and inflammation of HASMCs, and was applied to treat ASMCs in vitro for subsequent experiments.…”

Section: Discussionmentioning

confidence: 76%

Role of NEAT1/MiR-9-5p/SLC26A2 Pathway on Human Airway Smooth Muscle Cell

Wang

Chi

et al. 2021

Yonsei Med J

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Purpose Asthma is a serious inflammatory disease of the respiratory system in which airway smooth muscle cells (ASMCs) play a key role. This study aimed to investigate the expression of SLC26A2 in human ASMCs (HASMCs) and the regulatory mechanism of SLC26A2 in the proliferation and inflammatory factor production of HASMCs. Materials and Methods We obtained the asthma-associated differential mRNA SLC26A2 by bioinformatics analysis in childhood acute asthma samples. To investigate its role in airway inflammation and airway remodeling, we treated HASMCs with platelet-derived growth factor (PDGF) in an in vitro model and determined SLC26A2 expression in cells using western blotting. Cell proliferation was detected by MTT and EdU assays, and cell contractile phenotype marker proteins were measured. Cell migration and production of inflammatory factors were determined by Transwell and ELISA assays. Additionally, the upstream regulatory miRNA and LncRNA of SLC26A2 were identified by bioinformatics, luciferase reporter gene, and RIP analyses. Results SLC26A2 was significantly upregulated in bioinformatics analysis of pediatric asthma-related sample. PDGF treatment up-regulated SLC26A2 expression in HASMCs, whereas the knockdown of SLC26A2 inhibited PDGF-stimulated proliferation, migration, and production of inflammatory factors, and enhanced the expression of cell contractile phenotype marker proteins in HASMCs. Luciferase reporter and RIP experiments validated that NEAT1 targeted miR-9-5p to regulate SLC26A2, thereby influencing the biological function of PDGF-induced HASMCs. Conclusion These findings indicate that NEAT1-mediated miR-9-5p targeting of SLC26A2 inhibits the PDGF-induced proliferation and production of inflammatory factors in HASMCs. These findings highlight potential therapeutic targets for asthma and airway inflammation.

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“…19 In line with the previous studies, our results proved that the expressions of α-SMA and calponin were markedly decreased in the PDGFinduced ASMCs, while increased in the cells pretreated with salidroside. 20,21 We found that the PDGF-induced expressions of fibronectin and Col I were decreased after salidroside treatment. 19 ECM proteins are importantly involved in regulating phenotype and function of ASMCs.…”

Section: Discussionmentioning

confidence: 83%

Salidroside inhibits platelet‐derived growth factor–induced proliferation and migration of airway smooth muscle cells

Tian

et al. 2018

J of Cellular Biochemistry

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Abnormal proliferation and migration of airway smooth muscle cells (ASMCs) have been found to be important for the airway remodeling during the pathogenesis of asthma. Salidroside a bioactive glucoside that exerts antitumor activity via inhibiting the cell proliferation and migration of cancer cells. The aim of the current study was to evaluate the effects of salidroside on the proliferation and migration of ASMCs. Our results showed that salidroside inhibited the proliferation and migration of ASMCs in response to platelet‐derived growth factor (PDGF) stimulation. Salidroside markedly attenuated the PDGF‐induced production of matrix metalloproteinase 2 (MMP‐2) and MMP‐9 in ASMCs. The levels of contractile phenotype markers including smooth muscle α‐actin and calponin were reduced in response to PDGF stimulation, which was attenuated by salidroside pretreatment. Salidroside diminished the increase in the expression levels of type I collagen and fibronectin in PDGF‐stimulated ASMCs. Furthermore, salidroside blocked the PDGF‐induced activation of the nuclear factor‐κB (NF‐κB) pathway in ASMCs. The results suggested that salidroside functionally regulated the proliferation, migration, phenotype plasticity, and extracellular matrix deposition in PDGF‐induced ASMCs and the NF‐κB pathway might be implicated in the effects of salidroside on ASMCs induced by PDGF.

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Casticin inhibits PDGF-induced proliferation and migration of airway smooth muscle cells

Cited by 11 publications

References 27 publications

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

Role of NEAT1/MiR-9-5p/SLC26A2 Pathway on Human Airway Smooth Muscle Cell

Salidroside inhibits platelet‐derived growth factor–induced proliferation and migration of airway smooth muscle cells

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