Catabolism and Distribution of Serum‐albumin in the Dog

Wetterfors, J

doi:10.1111/j.0954-6820.1965.tb01826.x

Cited by 15 publications

(10 citation statements)

References 23 publications

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“…Thus in dogs, Wetterfors (1965) found by the two procedures EV/V ratios of 1·1 (0·8-1·2) and 1·4 (1,1-1,8), respectively. In a series of twenty humans, Beeken, Volwiler, Goldsworthy, Garby, Reynolds, Stogsdill & Stemler (1962) obtained a ratio of 1·3 (0'9-1'7) by the first method and 1·8 (1·2-2·8) by the second method.…”

Section: Discussionmentioning

confidence: 79%

Extravascular Albumin in Human Tissues

1970

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1. Extravascular albumin in human tissue was determined by direct immunochemical analysis. Samples of muscle, skin and intestinal tract obtained at surgery were extracted with a solution of 0·9% sodium chloride plus 0·1% desoxycholate and total albumin of the extracts was determined by radial diffusion in agarose plates containing antibody to human albumin. Occluded blood in tissues was estimated from the haemoglobin content of the extracts and the albumin due to blood calculated. This was used to obtain by difference the extravascular albumin content of the tissues. The extravascular albumin content of muscle and skin from seven patients was (mean and range) 3·6 (2·7-6·4) and 8·4 (6·5-12) mg/g, respectively. The albumin content of intestinal tissue (five patients) ranged from 4·3 to 9·0 mg/g (mean 6·3), and of stomach wall (three patients), from 3·5 to 5·4 mg/g (mean 4·4). Total extravascular albumin in humans is estimated to be at least 4 g/kg.2. The extravascular (EV) albumin of human tissue is similar to that of rats. It is concluded that calculation of the extravascular albumin from the intercepts and slopes of the decay curve of injected radioactive iodine-labelled albumin leads to underestimation of the extravascular mass by 20-40%. The validity of the conventional multicompartmenta1 model of albumin metabolism in the human is questioned.In the previous paper (Katz, Bonorris, Golden & Sellers, 1970) we have estimated by extraction and direct analysis the albumin content of rat tissues. We found that the extravascular albumin content determined directly is much greater than that calculated by isotopic methods, based on the assumption of the multicompartmental model of plasma albumin. It was of interest to determine directly the extravascular albumin content of human tissue. We report here that the extravascular albumin content of muscle and skin in humans is similar to that of rats. Our findings indicate that calculation of the mass of extravascular albumin pool from isotopic analysis is questionable.

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Section: Discussionmentioning

confidence: 79%

Extravascular Albumin in Human Tissues

1970

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“…The literature is listed in the reviews of Rothschild, Oratz & Schreiber (1969), Wetterfors (1965a), and the text of Schultze & Heremans (1966). The investigations were often incomplete and the results conflicting.…”

Section: Extravascular Albumin Massmentioning

confidence: 99%

“…The albumin content of muscle is reported to be 3·1 mgjg, a value in reasonable agreement with our own. Wetterfors (1965b), using a double isotope procedure, determined the albumin content of several tissues of dogs 5-8 days after injection. The muscles contained 3-4 mgjg and the gastrointestinal tract 7-10 mgjg, values very similar to our findings in rats.…”

Section: Extravascular Albumin Massmentioning

confidence: 99%

“…Albumin distribution has been calculated by several isotopic methods, and it has been estimated that in most species from 50 to 60% of body albumin is extravascular (Schultze & Heremans, 1966;Wetterfors, 1965b). We have observed the remarkable stability of circulating albumin in rats despite massive rapid losses of albumin from renin-induced proteinuria (Katz, Sellers & Bonorris, 1964).…”

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Extravascular Albumin Mass and Exchange in Rat Tissues

et al. 1970

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1. Extravascular albumin in carcass, skin and gut of rats was extracted and the albumin content estimated by several methods. Assay by electrophoresis on acrylamide gel, by immunodiffusion and by radioimmunoassay were in essential agreement. The method used previously, precipitation with antibody followed by alcoholTeA extraction, underestimates the amount of albumin in tissue extracts, because extraction from the antibody precipitate is not complete. This method is valid, however, for specific activity determination.2. Normal rats contain from 500 to 650 mg of albumin per 100 g body weight. Of this, 20-25% is in the circulation, 3~0% in the carcass (mainly but not exclusively muscle), 20-25% in skin and 10% in gut.3. The extracellular water of muscle, carcass, skin and gut was estimated from the distribution of mannitol and sulphate. With the exception of gut, both methods agreed closely. Extracellular, extravascular water constitutes about 23% of the body weight of 150-200 g rats. The extracellular water in muscle is about 20% and in skin, 40%. In gut the extracellular water cannot be estimated reliably by these compounds.4. Muscle contains about 3·5 mg/g of extravascular albumin; skin and gut, 7-8 mg/g. The concentration ofextravascular albumin in extracellular water of muscle and skin is 16-20 mg/ml, or 50-60% of the concentration in plasma. In the small intestine the concentration of albumin is higher, possibly similar to that in plasma.5. In rats with severe aminonucleoside nephrosis, body albumin was depleted to 100-200 mg/IOOg. Of this, 15-25% was in plasma, 50% in carcass, and about 15% in skin. Ascitic fluid contained only a few mg of albumin.6. The specific activity of extravascular albumin of tissues was followed after intravascular injection of 125 1_ or 1311-labelled albumin. The specific activity of carcass albumin increases rapidly, becoming equal to that in plasma after less than

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“…As so far data on the relationship between LBM and indicators of albumin distribution and meta bolism are lacking, we decided to answer the question whether LBM is a K udli£ ka/R ath better reference standard for albumin metabolism than hitherto used reference units, i.e. body weight, height or body surface [1][2][3]. In the present paper we are presenting the first results of this investigation.…”

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confidence: 99%

Albumin Metabolism as a Function of Lean Body Mass

Kudlicka¹,

Rath²

1972

Ann Nutr Metab

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In 13 subjects with different body weight, the body density was assessed by hydrostatic weighing and the metabolism and distribution of albumin labelled with radioactive iodine. Between the amount of thus estimated lean body mass (LBM) and parameters of albumin metabolism, important correlations were found. The relationship of albumin catabolism and amount of LBM is significant (r = +0.6494; 0.05 > p > 0.01), similarly as the relationship between total albumin and LBM. It is of interest that between 20 and 40 years there is a continuous decline in the catabolic rate of albumin per unit of LBM and thus the decline of the metabolic rate is not mediated by the changing ratio of body fat. It was demonstrated that the lean body mass is also the most suitable reference standard for the evaluation of albumin metabolism.

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Catabolism and Distribution of Serum‐albumin in the Dog

Cited by 15 publications

References 23 publications

Extravascular Albumin in Human Tissues

Extravascular Albumin in Human Tissues

Extravascular Albumin Mass and Exchange in Rat Tissues

Albumin Metabolism as a Function of Lean Body Mass

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