Extravascular Albumin in Human Tissues

Abstract

1. Extravascular albumin in human tissue was determined by direct immunochemical analysis. Samples of muscle, skin and intestinal tract obtained at surgery were extracted with a solution of 0·9% sodium chloride plus 0·1% desoxycholate and total albumin of the extracts was determined by radial diffusion in agarose plates containing antibody to human albumin. Occluded blood in tissues was estimated from the haemoglobin content of the extracts and the albumin due to blood calculated. This was used to obtain by difference the extravascular albumin content of the tissues. The extravascular albumin content of muscle and skin from seven patients was (mean and range) 3·6 (2·7-6·4) and 8·4 (6·5-12) mg/g, respectively. The albumin content of intestinal tissue (five patients) ranged from 4·3 to 9·0 mg/g (mean 6·3), and of stomach wall (three patients), from 3·5 to 5·4 mg/g (mean 4·4). Total extravascular albumin in humans is estimated to be at least 4 g/kg.2. The extravascular (EV) albumin of human tissue is similar to that of rats. It is concluded that calculation of the extravascular albumin from the intercepts and slopes of the decay curve of injected radioactive iodine-labelled albumin leads to underestimation of the extravascular mass by 20-40%. The validity of the conventional multicompartmenta1 model of albumin metabolism in the human is questioned.In the previous paper (Katz, Bonorris, Golden & Sellers, 1970) we have estimated by extraction and direct analysis the albumin content of rat tissues. We found that the extravascular albumin content determined directly is much greater than that calculated by isotopic methods, based on the assumption of the multicompartmental model of plasma albumin. It was of interest to determine directly the extravascular albumin content of human tissue. We report here that the extravascular albumin content of muscle and skin in humans is similar to that of rats. Our findings indicate that calculation of the mass of extravascular albumin pool from isotopic analysis is questionable.

Clinical Pharmacokinetics in Patients with Spinal Cord Injuries

“…This increase may be partially attributed to the redistribution of albumin-rich intravascular fluid to extravascular sites (Katz et al 1970) where this hydrophilic aminoglycoside, although only 10 to 20% bound to serum albumin (Regamey et al 1973), accumulates. Plantin et al (1971) studied the extravascular distribution of albumin in paraplegic subjects.…”

Chemical changes in bovine serum albumin photoinduced by pterin

“…Especially, serum albumins are the most abundant plasma proteins, and their main biological function is the transport of a wide variety of molecules [3]. In addition, albumin is present in the human skin [4], where there is an autocrine synthesis and regulation [5]. It has been reported that in patients affected by vitiligo, epidermal albumin oxidation takes place [6].…”

Photosensitization of peptides and proteins by pterin derivatives

“…In addition, albumin is present in human skin [36], where there is an autocrine synthesis and regulation [37]. It has been reported that in patients affected by vitiligo, epidermal albumin oxidation takes place, but the mechanism of this process has not been elucidated [38].…”