Catabolism of sulfamate by Mycobacterium sp. CF1

Fulton, Craig K.; Cooper, Ronald A.

doi:10.1111/j.1462-2920.2005.00719.x

Cited by 23 publications

(8 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chromosomal DNA was extracted using Qiagen DNA extraction kit as described by the manufacturer. PCR amplification was carried out using universal primers to amplify the 16S rDNA gene as described by Fulton and Cooper [21].…”

Section: Pcr Amplification Of 16s Rdna Genementioning

confidence: 99%

Further Analysis of Burkholderia pseudomallei MF2 and Identification of Putative Dehalogenase Gene by PCR

Edbeib¹,

Wahab

Huyop

et al. 2020

Indones. J. Chem.

View full text Add to dashboard Cite

Halogenated organic compounds are extensively and widely used as pesticides, herbicides, and antibiotics that contribute to the pollution. This research was aimed to further analyze and characterize a bacterium that has the ability to utilize 2,2dichloropropionic acid (2,2-DCP) as a model to study dehalogenase enzyme production. Microscopic observation, biochemical tests and PCR technique were carried out in order to characterize the isolated bacterium. Strain MF2 showed its ability to grow on 10 mM 2,2-DCP liquid minimal medium with doubling time of 13 h with maximum chloride ion released of 19.8 molCl -/mL. The 16S rDNA analysis suggested that strain MF2 belongs to the genus Burkholderia. This was supported by the microscopic observation and biochemical tests. Dehalogenase gene was observed when using only primers dehIfor1 and dehIrev2 derived from group I deh PCR primer sequences, whereas no amplification using dhlB-314-forward and dhlB-637-reverse (group II dehalogenase) and haloacetate dehalogenase (H2-1157-forward and H2-1662-reverse) PCR primer sequences. The results suggested that, possibly, dehalogenase from MF2 was related to group I deh. In conclusion, strain MF2 showed the ability to utilize 2,2-DCP as sole source of carbon and energy. Further analysis revealed the MF2 strain consisted of dehalogenase gene that could be used for degradation of man-made halogenated compounds present in the environment. Using existing dehalogenase PCR primers, it was possible to amplify the dehalogenase genes sequence.

show abstract

Section: Pcr Amplification Of 16s Rdna Genementioning

confidence: 99%

Further Analysis of Burkholderia pseudomallei MF2 and Identification of Putative Dehalogenase Gene by PCR

Edbeib¹,

Wahab

Huyop

et al. 2020

Indones. J. Chem.

View full text Add to dashboard Cite

show abstract

“…Mycobacterium soil isolate (Fulton & Cooper, 2005), but the corresponding gene or genes were never cloned. S-N bonds in heparan sulfate can be cleaved by the NSulf heparan N-sulfatase from Pedobacter heparinus (Myette et al, 2009) and by human sulfamidase (Scott et al, 1995), both of which are members of the arylsulfatase family (Fig.…”

Section: T Lindermentioning

confidence: 99%

Genomics of alternative sulfur utilization in ascomycetous yeasts

Linder

2012

Microbiology

View full text Add to dashboard Cite

“…The polymerase chain reaction has been performed in order to amplify the 16S rRNA of the isolates. The universal primers used were Fd1(27F) (5-AGA GTT TGA TCC TGG CTC AG-3) and rP1(1422R) (5-GGT TAC CTT GTT ACG ACT T) [25]. The 16S rRNA gene amplification was conducted for 30 cycles, each of which was set as a first denaturation phase of 94ºC for 5 minutes, proceeded by denaturation of 94ºC for 1 minute, annealing of 55°C for 1 minute and final extension of 72°C for 10 minutes.…”

Section: S Rrna Gene Analysismentioning

confidence: 99%

Identification and Characterization of a 2,2-Dichloropropionic Acid (2,2-DCP) Degrading Alkalotorelant Bacterium Strain BHS1 Isolated from Blue Lake, Turkey

Wahhab

Anuar

Wahab

et al. 2020

J.Trop.Life.Science

View full text Add to dashboard Cite

An acid, 2,2-dichloropropionic acid (2,2-DCP) is an active ingredient in herbicide (Dalapon®). Using 2,2-DCP as a model substrate, an alkalotolerant bacterium was successfully isolated from the Blue Lake, Turkey. This bacterium is a potential bioremediation agent of recalcitrant xenobiotic halogenated compounds. This study aimed to prove the efficacy of the alkalotolerance Bacillus megaterium BHS1 in degrading 2,2-DCP as the sole source of carbon. Biolog GEN III system and 16S rRNA analysis were used for the identification of the bacterium. It was discovered that the strain BHS1 is Bacillus megaterium, and the bacterium that was observed to thrive in alkaline conditions (pH 7.0−14.0), supplemented with varying concentrations of 2,2-DCP (from 20 to 60 mM). Growth of strain BHS1 was exceptional in 40 mM of 2,2-DCP at pH 9, corresponding to a cell doubling time of 17.7 hour, whereas was fully inhibited at 50 mM 2,2-DCP. Since halogenated pollutants can make their way into highly alkaline environments, therefore, identifying threshold levels of strain BHS1 with respect to alkaline-tolerance and maximum level of 2,2-DCP may prove pertinent. This is to ensure that an optimal environment is created for the bacteria to degrade 2,2-DCP-contaminated water. In addition, this is the first study exploring a Bacillus species isolated from an alkaline environment adept in utilizing 2,2-DCP as a sole source of carbon. Hence, the ability of this strain to degrade other types of haloalkanoic acids constitutes a worthy future study.

show abstract

Catabolism of sulfamate by Mycobacterium sp. CF1

Cited by 23 publications

References 13 publications

Further Analysis of <i>Burkholderia pseudomallei</i> MF2 and Identification of Putative Dehalogenase Gene by PCR

Further Analysis of <i>Burkholderia pseudomallei</i> MF2 and Identification of Putative Dehalogenase Gene by PCR

Genomics of alternative sulfur utilization in ascomycetous yeasts

Identification and Characterization of a 2,2-Dichloropropionic Acid (2,2-DCP) Degrading Alkalotorelant Bacterium Strain BHS1 Isolated from Blue Lake, Turkey

Contact Info

Product

Resources

About