Catabolismo muscular en pacientes con enfermedad pulmonar obstructiva crónica

The aim of the present work is to determine new biomarkers of the biological effects of hesperidin in orange juice (OJ) applying a non-targeted metabolomics approach validated by targeted metabolomics analyses of compliance biomarkers. Methods and Results: Plasma/serum and urine targeted (HPLC-MS/MS) and untargeted ( 1 H-NMR) metabolomics signatures are explored in a subsample with pre-and stage-1 hypertension subjects of the CITRUS study (N = 159). Volunteers received 500 mL day −1 of control drink, OJ, or hesperidin-enriched OJ (EOJ) for 12-weeks. A 6-h postprandrial study is performed at baseline. Targeted analyses reveals plasma and urine hesperetin 7-O--d-glucuronide as the only metabolite differing between OJ and EOJ groups after 12-weeks consumption, and in urine is correlated with a decreased systolic blood pressure level. The non-targeted approach shows that after single dose and 12-weeks consumption of OJ and EOJ change several metabolites related with an anti-inflammatory and antioxidant actions, lower blood pressure levels and uremic toxins. Conclusions: Hesperetin 7-O--d-glucuronide can be a candidate marker for distinguishing between the consumption of different hesperidin doses at 12-weeks consumption as well as a potential agent mediating blood pressure reduction. Moreover, changes in different endogenous metabolites can explain the mechanisms of action and the biological effects of hesperidin consumption.

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Effects of Hesperidin Consumption on the Cardiovascular System in Pre‐ and Stage 1 Hypertensive Subjects: Targeted and Non‐Targeted Metabolomic Approaches (CITRUS Study)

Pla‐Pagà

Pedret

Valls

et al. 2021

Molecular Nutrition Food Res

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Tubular dysfunction impairs renal excretion of pseudouridine in diabetic kidney disease

Mathew,

Kayampilly,

Byun

et al. 2024

American Journal of Physiology-Renal Physiology

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Plasma nucleosides-pseudouridine (PU) and N2N2-dimethyl guanosine (DMG) predict the progression of type 2 diabetic kidney disease (DKD) to end-stage renal disease, but the mechanisms underlying this relationship are not well-understood. We used a well-characterized model of type 2 diabetes ( db/db mice) and control non-diabetic mice ( db/m mice) to characterize the production and excretion of PU and DMG levels using liquid chromatography-mass spectrometry. The fractional excretion of PU and DMG was decreased in db/db mice compared to control mice at 24 weeks before any changes to renal function. We then examined the dynamic changes in nucleoside metabolism using in vivo metabolic flux analysis (MFA) with the injection of labeled nucleoside precursors. MFA revealed significant decreases in the ratio of urine-to-plasma labeling of PU and DMG in db/db mice compared to db/m mice indicating significant tubular dysfunction in diabetic kidney disease. We observed that the gene and protein expression of the renal tubular transporters involved with nucleoside transport in diabetic kidneys in mice and humans was reduced. In conclusion, this study strongly suggests that tubular handling of nucleosides is altered in early DKD, in part explaining the association of PU and DMG with human DKD progression observed in previous studies.

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Catabolismo muscular en pacientes con enfermedad pulmonar obstructiva crónica

Cited by 2 publications

References 30 publications

Effects of Hesperidin Consumption on the Cardiovascular System in Pre‐ and Stage 1 Hypertensive Subjects: Targeted and Non‐Targeted Metabolomic Approaches (CITRUS Study)

Effects of Hesperidin Consumption on the Cardiovascular System in Pre‐ and Stage 1 Hypertensive Subjects: Targeted and Non‐Targeted Metabolomic Approaches (CITRUS Study)

Tubular dysfunction impairs renal excretion of pseudouridine in diabetic kidney disease

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