Catalase Activity of Psychrophilic Bacteria Grown at 2 and 30 C

A newly isolated Pseudomonas fragi P121 strain in a soil sample taken from the Arctic Circle is able to produce trehalose. The P121 strain was able to grow at temperatures ranging from 4 to 25 °C, had an optimum pH of 6.5, and an optimum salt concentration of 2 %. The P121 strain had a survival rate of 29.1 % after being repeatedly frozen and thawed five times, and a survival rate of 78.9 % when placed in physiological saline for 15 days at 20 °C after cold shock, which is far higher than the type strain Pseudomonas fragi ATCC 4973. The P121 strain could produce 2.89 g/L trehalose, which was 18.6 % of dry cell weight within 52 h in a 25 L fermention tank using the malt extract prepared from barley as medium at 15 °C, while only 11.8 % of dry cell weight at 20 °C. These results suggested that cold stress promoted the strain producing trehalose. It is the first reported cold-tolerant bacterium that produces trehalose, which may protect cells against the cold environment.

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Three-dimensional Reconstruction of the Surface Protein of the Extremely Thermophilic Archaebacterium Archaeoglobus fulgidus

Kessel

Volker

Santarius

et al. 1990

Systematic and Applied Microbiology

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Catalase Activity of Psychrophilic Bacteria Grown at 2 and 30 C

Abstract: Catalase activity was measured in resting-cell suspensions of psychrophilic bacteria grown at 2 and at 30 C. Enzyme activity decreased in both cell-suspension types as harvest age increased. At comparable physiological age, cells grown at 2 C had more catalase than cells grown at 30 C.

Cited by 7 publications

References 20 publications

Temperature range for formic hydrogenlyase induction and activity in psychrophilic and mesophilic bacteria

Temperature range for formic hydrogenlyase induction and activity in psychrophilic and mesophilic bacteria

Cold stress promoting a psychrotolerant bacterium Pseudomonas fragi P121 producing trehaloase

Three-dimensional Reconstruction of the Surface Protein of the Extremely Thermophilic Archaebacterium Archaeoglobus fulgidus

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