Catalase is a key enzyme in seed recovery from ageing during priming

Kibinza, Serge; Bazin, Jérémie; Bailly, Christophe; Farrant, Jill M.; Corbineau, Françoise

doi:10.1016/j.plantsci.2011.06.003

Cited by 183 publications

(119 citation statements)

References 51 publications

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…In parallel, the CAT acts to reduce the H 2 O 2 to H 2 O and O 2 , preventing the generation of more reactive radicals such as OH˙ (Perl-Treves and Perl, 2002;Kibinza et al, 2011). CAT and SOD are the most efficient enzymes of antioxidant metabolism, since their combined actions convert hazardous molecules into H 2 O and O 2 , avoiding damage to cellular components (Scandalios, 1993).…”

Section: Discussionmentioning

confidence: 99%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

View full text Add to dashboard Cite

This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA 3 or 0.2 µM NAA, sucrose at 15 or 30 g L -1 , in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA 3 and 15 g L -1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L -1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.Keywords: enzymatic activity, oxidative stress, micropropagation, proline. Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) ResumoEste trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA 3 ou 0,2 µM de ANA, 15 ou 30 g L -1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA 3 e 15 g L -1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L -1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.Palavras-chave: atividade enzimática, estresse oxidativo, micropropagação, prolina.

show abstract

Section: Discussionmentioning

confidence: 99%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The expression levels of the SOD ( Figure 2) and CAT3 (Figure 3) transcripts were similar at the more advanced maturation stages. According to Kibinza et al (2011), among the carrier enzymes that participate in ROS removal, SOD removes superoxide (O 2 • -), thus converting it to hydrogen peroxide (H 2 O 2 ), which, in turn, can be decomposed by CAT to produce oxygen and water. H 2 O 2 is largely responsible for oxidative stress and may contribute to regulation of the coffee berry maturation process (Brennan and Frenkel, 1977).…”

Section: Discussionmentioning

confidence: 99%

“…Enzymes, including catalase (CAT) and superoxide dismutase (SOD), are protective systems that take part in removing reactive oxygen species (ROS) (Kibinza et al, 2011). The polyphenol oxidase (PPO) enzyme in coffee berries is bonded to the cell membranes and, in the presence of oxygen, causes oxidation of certain compounds (Lupetti et al, 2003) such as quinones (Pimenta et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Gene expression of antioxidant enzymes and coffee seed quality during pre- and post-physiological maturity

Santos¹,

Caixeta²,

Clemente³

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT.Seeds collected at different maturation stages vary in physiological quality and patterns of protective antioxidant systems against deterioration. In this study we investigated the expression of genes that codify catalase (CAT), dismutase superoxide (SOD), and polyphenol oxidase (PPO) during the pre-and post-physiological maturation phases in whole seeds and in endosperms and embryos extracted from the seeds. Coffea arabica L. berries were collected at the green, yellowish-green, cherry, over-ripe, and dry stages, and the seeds were examined physiologically. The transcription levels of the genes were quantified by quantitative real-time polymerase chain reaction using coffee-specific primers. The highest level of SOD expression was observed in the endosperm at the cherry and over-ripe stages; in addition, these seeds presented the greatest physiological quality (assessed via germination test). The highest CAT3 transcript expression was observed at the green stage in whole seeds, and at the green and over-ripe stages in the embryos and endosperms. High expression of the PPO transcript was observed at the green and yellowish-green stages in whole seeds. In embryos and endosperms, peak expression of the PPO transcript was observed at the green stage; subsequently, peaks at the cherry and over-ripe stages were observed. We concluded that the expression patterns of the SOD and CAT3 transcripts were similar at the more advanced maturation stages, which corresponded to enhanced physiological seed quality. High expression of the PPO transcript at the over-ripe stage, also observed in the embryos and endosperms at the cherry stage, coincided with the highest physiological seed quality.

show abstract

“…Therefore, to maintain seed viability, the regulation of ROS levels by enzymatic and non-enzymatic (antioxidant) activities, in conjunction with DNA repair mechanisms, should be at appropriate levels in the embryo (Kibinza, Bazin, Bailly, Farrant, & Corbineau, 2011;Donà et al, 2013). In seeds, the main enzymes present that scavenge these radicals are superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione peroxidase (GPX), and peroxiredoxin (PrxR) (Mittler, 2002;Rajjou & Debeaujon, 2008).…”

Section: Introductionmentioning

confidence: 99%

<b>Lipid peroxidation and antioxidant enzymes of <i>Jatropha curcas</i> L. seeds stored at different maturity stages

et al. 2018

View full text Add to dashboard Cite

ABSTRACT. The objective of the present work was to investigate the antioxidant enzyme activities and the occurrence of lipid peroxidation during storage of J. curcas seeds obtained from fruits at different maturity stages. Seeds extracted from fruits collected at three stages of maturity were used, i.e., yellow, brownish-yellow, and brown (dry fruits). The seeds were subsequently stored for 18 months in Kraft paper bags in a laboratory environment. Initially as well as every three months, the seeds were evaluated for germination and electrical conductivity, and the seed embryos were evaluated for lipid peroxidation; protein content; and superoxide dismutase, peroxidase, and ascorbate peroxidase enzyme activity at the same time points. In general, the seeds from the three stages of fruit maturity did not differ regarding germination throughout the entire period of storage, although a reduction in viability was observed after nine months of storage. The electrical conductivity increased during storage and was greater for the seeds obtained from brown fruits. There was a reduction in protein content and in enzyme activities in the seed embryo, except for superoxide dismutase. No relationship was observed between reduction in seed viability and lipid peroxidation. Thus, J. curcas seeds with high initial quality can be stored under environmental conditions for up to 9, 15 and 15 months for brown (dry), yellow and brownish-yellow fruits, respectively, without a significant reduction in germination but with a reduction in seed vigour.Keywords: physic nut, seed maturation and deterioration, enzyme activity, malondialdehyde content.Peroxidação de lipídios e enzimas antioxidativas em sementes de Jatropha curcas L. armazenadas em diferentes estágios de maturação RESUMO. O objetivo do presente trabalho foi avaliar a atividade de enzimas antioxidativas e a peroxidação de lipídios durante o armazenamento de sementes de J. curcas em diferentes estádios de maturação. Foram utilizadas sementes extraídas de frutos colhidos com coloração externa da casca amarela, amarela-marrom e marrom (frutos secos). As sementes foram armazenadas por 18 meses, em embalagem de papel Kraft em ambiente de laboratório. Inicialmente e a cada três meses, as sementes foram avaliadas quanto à germinação e condutividade elétrica, e os embriões das sementes foram avaliados quanto a peroxidação de lipídios, conteúdo de proteínas e atividade das enzimas superóxido dismutase, peroxidases e peroxidase do ascorbato. Em geral, as sementes dos três estádios de maturação dos frutos não diferiram quanto à germinação durante todo o período de armazenamento, embora tenha sido observada uma redução da germinação a partir de nove meses de armazenamento. Houve redução no conteúdo de proteína e na atividade das enzimas antioxidantes no embrião das sementes dos três estádios de maturação, com exceção da superóxido dismutase. Não foi observada relação entre a redução da viabilidade das sementes e a peroxidação de lipídios. Sementes de J. curcas com elevada qualidade inici...

show abstract

Catalase is a key enzyme in seed recovery from ageing during priming

Cited by 183 publications

References 51 publications

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Gene expression of antioxidant enzymes and coffee seed quality during pre- and post-physiological maturity

<b>Lipid peroxidation and antioxidant enzymes of <i>Jatropha curcas</i> L. seeds stored at different maturity stages

Contact Info

Product

Resources

About