Catalase Regulates Cell Growth in HL60 Human Promyelocytic Cells: Evidence for Growth Regulation by H<sub>2</sub>O<sub>2</sub>

Hachiya, Misao; Akashi, Makoto

doi:10.1667/rr3306

Cited by 34 publications

(24 citation statements)

References 79 publications

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“…11 Cells were seeded at an initial concentration of 5 Â 10 4 cells/well in a medium with or without various concentrations of H 2 O 2 in a 96-well microplate and cultured for 48 h. Then tetrazolium salt WST-1 was added to the cultures and incubated at 37 1C for 1 h. The production of formazan was measured by microplate reader (Bio-Rad Model 3550, Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 480 nm.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

“…6,9,10 Previously, we showed that ROS regulate cell growth in human promyelocytes HL60. 11 Recently, there is a growing body of evidence regarding the ROS signal playing an important role in hematopoietic systems. The regulation of oxidative stress by ROS has been shown to be necessary for the maintenance of the capacity of hematopoietic stem cells to self-renew.…”

Section: Introductionmentioning

confidence: 99%

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Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger

Yamamoto¹,

Sakaguchi²,

Hachiya³

et al. 2008

Leukemia

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Human promonocytic cell line U937 cells can be induced to differentiate into macrophages by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA treatment induced the expression of the monocytic differentiation markers CD11b and CD36, with concomitant morphological changes. Moreover, TPA enhanced reactive oxygen species (ROS) generation in these cells, and phagocytic ability was also stimulated during differentiation. The antioxidant agent N-acetyl-L-cysteine inhibited the TPA-induced differentiation of U937 cells. TPA treatment decreased the expression level of catalase, which catalyzes the decomposition of hydrogen peroxide (H 2 O 2 ) to H 2 O and O 2 . In contrast, TPA increased the level of manganese superoxide dismutase, which catalyzes the dismutation of superoxide into H 2 O 2 and O 2 without affecting the levels of copper-zinc superoxide dismutase or glutathione peroxidase 1, which removes H 2 O 2 using glutathione as substrate. Treatment of U937 cells with catalase inhibited the enhancement of ROS generation induced by TPA, and blocked the TPA-induced differentiation of U937 cells. Human promyelocytic cell line HL60 cells were also induced to differentiate into macrophages by TPA. However, HP100-1 cells, its variant cell line overexpressing catalase, were resistant to TPA-induced differentiation. Our results suggest that catalase inhibits monocytic differentiation by TPA; the decrease in catalase level and the accumulation of H 2 O 2 are significant events for monocyte/ macrophage differentiation by TPA.

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Section: Cytotoxicity Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger

Yamamoto¹,

Sakaguchi²,

Hachiya³

et al. 2008

Leukemia

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“…Proteins identified as more significantly altered between the different AML subtypes belonged to the groups of suppressor genes, metabolic enzymes, antioxidants, structural proteins and signal transduction mediators. Among them, catalase, an antiapoptotic enzyme, 72 and annexin I, a calcium-and phospholipid-binding protein that specifically regulates signaling components of the Ras/MEK/ERK signaltransduction pathway, 73 were found to be expressed in M1, M3 and M5 AML samples at higher levels than in normal monocytes and than in other AML subtypes. On the contrary, tropomyosin, a family of cytoskeletal proteins whose inhibition contribute to the invasive and metastatic properties of cancer cells, 74 was found concomitantly reduced in these AML subtypes.…”

Section: Role Of Tissue Factor In Hematological Malignanciesmentioning

confidence: 99%

Tissue factor as an effector of angiogenesis and tumor progression in hematological malignancies

et al. 2006

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“…For the precise evaluation of ROS production immediately following X-irradiation, we labeled the cells with H 2 DCFDA prior to AsA and X-irradiation treatments (27,28). In brief, cells were incubated at 37˚C with 5 µM H 2 DCFDA in PBS(-) for 15 min.…”

Section: Measurement Of Intracellular Rosmentioning

confidence: 99%

Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species

et al. 2013

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Abstract. Ascorbic acid (AsA) treatment is expected to be a potential cancer therapy strategy with few side-effects that can be used alone or in combination with chemotherapy. However, the combination of AsA, a free radical scavenger, with radiation is not clearly understood; conflicting data are reported for cancer cell death. We conducted this study to determine the effect of AsA treatment combined with X-irradiation and the role of reactive oxygen species (ROS) in HL-60 human promyelocytic leukemia cells. Additive cytotoxic effects were observed when the cells were exposed to 2 Gy X-irradiation after 2.5 mM AsA treatment. When catalase was added to the culture with AsA alone, the cytotoxic effects of AsA disappeared. X-irradiation increased intercellular ROS levels and mitochondrial superoxide levels. By contrast, AsA alone and in combination with X-irradiation decreased ROS levels. However, in the presence of catalase neutralizing H 2 O 2 , AsA alone or in combination with X-irradiation only slightly decreased the intercellular ROS. Moreover, AsA decreased the mitochondrial membrane potential, which is commonly associated with apoptosis. These results suggest that the reduction of ROS did not result from ROS scavenging by AsA, and AsA induced apoptosis through a ROS-independent pathway. This study reports that a combination of AsA with radiation treatment is effective in cancer therapy when considering ROS in cancer cells.

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Catalase Regulates Cell Growth in HL60 Human Promyelocytic Cells: Evidence for Growth Regulation by H₂O₂

Cited by 34 publications

References 79 publications

Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger

Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger

Tissue factor as an effector of angiogenesis and tumor progression in hematological malignancies

Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species

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Catalase Regulates Cell Growth in HL60 Human Promyelocytic Cells: Evidence for Growth Regulation by H2O2

Cited by 34 publications

References 79 publications

Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger

Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger

Tissue factor as an effector of angiogenesis and tumor progression in hematological malignancies

Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species

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Catalase Regulates Cell Growth in HL60 Human Promyelocytic Cells: Evidence for Growth Regulation by H₂O₂