Cataloguing and Selection of mRNAs Localized to Dendrites in Neurons and Regulated by RNA-Binding Proteins in RNA Granules

Ohashi, Rie; Shiina, Nobuyuki

doi:10.3390/biom10020167

Cited by 22 publications

(17 citation statements)

References 383 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…mRNA interactions with RNA binding proteins can also affect the translation of mRNA. In the hippocampus, mRNA interactions with RNA binding proteins are important for the localization of mRNA transcripts necessary for synaptic plasticity [ 86 ]. Although identification of the mechanism(s) impacted by acute sleep deprivation that affect protein synthesis will undoubtedly be a subject of great interest in future research, we hypothesize that sleep deprivation targets multiple processes as the mechanisms that result in down-regulation of transcripts available for translation will be different than the mechanisms that can increase the availability of transcripts for protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq

et al. 2020

View full text Add to dashboard Cite

Sleep deprivation is a global health problem adversely affecting health as well as causing decrements in learning and performance. Sleep deprivation induces significant changes in gene transcription in many brain regions, with the hippocampus particularly susceptible to acute sleep deprivation. However, less is known about the impacts of sleep deprivation on post-transcriptional gene regulation. To identify the effects of sleep deprivation on the translatome, we took advantage of the RiboTag mouse line to express HA-labeled Rpl22 in CaMKIIα neurons to selectively isolate and sequence mRNA transcripts associated with ribosomes in excitatory neurons. We found 198 differentially expressed genes in the ribosome-associated mRNA subset after sleep deprivation. In comparison with previously published data on gene expression in the hippocampus after sleep deprivation, we found that the subset of genes affected by sleep deprivation was considerably different in the translatome compared with the transcriptome, with only 49 genes regulated similarly. Interestingly, we found 478 genes differentially regulated by sleep deprivation in the transcriptome that were not significantly regulated in the translatome of excitatory neurons. Conversely, there were 149 genes differentially regulated by sleep deprivation in the translatome but not in the whole transcriptome. Pathway analysis revealed differences in the biological functions of genes exclusively regulated in the transcriptome or translatome, with protein deacetylase activity and small GTPase binding regulated in the transcriptome and unfolded protein binding, kinase inhibitor activity, neurotransmitter receptors and circadian rhythms regulated in the translatome. These results indicate that sleep deprivation induces significant changes affecting the pool of actively translated mRNAs.

show abstract

Section: Discussionmentioning

confidence: 99%

Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The CPEB proteins perform these functions as part of larger RNP complexes. A recent review [ 27 ] summarizes what is known about the composition of the RNP complexes in dendrites, which are associated with and/or regulated by the CPEB1 protein. CPEB1 interacts with 11 transcripts involved in long-term potentiation, memory formation, and synapse morphogenesis.…”

Section: Functions Of Cpeb Proteins In Brainmentioning

confidence: 99%

The role of CPEB family proteins in the nervous system function in the norm and pathology

et al. 2021

View full text Add to dashboard Cite

Posttranscriptional gene regulation includes mRNA transport, localization, translation, and regulation of mRNA stability. CPEB (cytoplasmic polyadenylation element binding) family proteins bind to specific sites within the 3′-untranslated region and mediate poly- and deadenylation of transcripts, activating or repressing protein synthesis. As part of ribonucleoprotein complexes, the CPEB proteins participate in mRNA transport and localization to different sub-cellular compartments. The CPEB proteins are evolutionarily conserved and have similar functions in vertebrates and invertebrates. In the nervous system, the CPEB proteins are involved in cell division, neural development, learning, and memory. Here we consider the functional features of these proteins in the nervous system of phylogenetically distant organisms: Drosophila, a well-studied model, and mammals. Disruption of the CPEB proteins functioning is associated with various pathologies, such as autism spectrum disorder and brain cancer. At the same time, CPEB gene regulation can provide for a recovery of the brain function in patients with fragile X syndrome and Huntington's disease, making the CPEB genes promising targets for gene therapy.

show abstract

“…This is the case for the cytoplasmic polyadenylation element binding protein 1 (CPEB1), which regulates polyA tail length and binds cytoplasmic polyadenylation elements (CPEs) in mRNA (Hake and Richter, 1994). CPEB1 also regulates synaptic plasticity (Alarcon et al, 2004) and binds to RNA granules in dendrites (Ohashi and Shiina, 2020). In primary astrocytes, Fabp7 mRNA (containing CPEs in its 3'UTR) co-immunoprecipitates with CPEB1 (Gerstner et al, 2012).…”

Section: The Molecular Bases Of Translation In Astrocytesmentioning

confidence: 99%

Local translation in perisynaptic and perivascular astrocytic processes – a means to ensure astrocyte molecular and functional polarity?

Mazaré

Oudart

Cohen-Salmon

2021

Journal of Cell Science

View full text Add to dashboard Cite

Together with the compartmentalization of mRNAs in distal regions of the cytoplasm, local translation constitutes a prominent and evolutionarily conserved mechanism mediating cellular polarization and the regulation of protein delivery in space and time. The translational regulation of gene expression enables a rapid response to stimuli or to a change in the environment, since the use of pre-existing mRNAs can bypass time-consuming nuclear control mechanisms. In the brain, the translation of distally localized mRNAs has been mainly studied in neurons, whose cytoplasmic protrusions may be more than 1000 times longer than the diameter of the cell body. Importantly, alterations in local translation in neurons have been implicated in several neurological diseases. Astrocytes, the most abundant glial cells in the brain, are voluminous, highly ramified cells that project long processes to neurons and brain vessels, and dynamically regulate distal synaptic and vascular functions. Recent research has demonstrated the presence of local translation at these astrocytic interfaces that might regulate the functional compartmentalization of astrocytes. In this Review, we summarize our current knowledge about the localization and local translation of mRNAs in the distal perisynaptic and perivascular processes of astrocytes, and discuss their possible contribution to the molecular and functional polarity of astrocytes.

show abstract

Cataloguing and Selection of mRNAs Localized to Dendrites in Neurons and Regulated by RNA-Binding Proteins in RNA Granules

Cited by 22 publications

References 383 publications

Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq

Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq

The role of CPEB family proteins in the nervous system function in the norm and pathology

Local translation in perisynaptic and perivascular astrocytic processes – a means to ensure astrocyte molecular and functional polarity?

Contact Info

Product

Resources

About