Catalytic behavior of pancreatic lipase in crowded medium for hydrolysis of medium-chain and long-chain lipid: An isothermal titration calorimetry study

The detoxification of ochratoxin A in wine and grape juice by different enzymes and evaluation of their effects on the quality

“…Pancreatic lipase was interfacial enzyme. The crowding of macromolecular, inducing of conformational and changing of other structural might enhance the intrinsic activity of enzyme (Y. Zhang et al, 2019). Therefore, a positive correlation was found between the OTA degradation rate and soluble solids content. Enzyme concentrationThe effect of enzyme concentration on OTA degradation was displayed in Figure 2b.…”

“…Pancreatic lipase was interfacial enzyme. The crowding of macromolecular, inducing of conformational and changing of other structural might enhance the intrinsic activity of enzyme (Y. Zhang et al, 2019). Therefore, a positive correlation was found between the OTA degradation rate and soluble solids content.…”

Structural characterization and hypolipidemic activity of Gracilaria lemaneiformis polysaccharide and its degradation products

“…The addition of macromolecules to alter the microscopic configuration of the enzyme molecule promoted the exposure of phenylalanine (Phe), tyrosine, (Tyr) and tryptophan (Trp) residues that were previously surrounded by other amino acid residues ( Zhang et al, 2019 ). Polysaccharide changed the microenvironment of lipase, leading to variation of quantum yield and fluorescence intensity, and the degree of this change was related to the degradation methods and concentration of polysaccharide.…”

“…Pancreatic lipase is mainly secreted by pancreatic acinar and stomach cells, which is the most important enzyme in the hydrolysis of dietary fat. It can hydrolyze triglyceride and cholesterol into glycerol monoesters and fatty acids, which are absorbed by small intestinal epithelial cells ( Zhang et al, 2019 ). While cholesterol esterase is a non-specific serine hydrolase, mainly from pancreas and mammary gland of higher mammals ( Aggarwal et al, 2016 , Zhao et al, 2021 ).…”

Plant Products and Their Inhibitory Activity Against Pancreatic Lipase

“…Activation of the enzyme occurs at water-oil interfaces to interact with water-insoluble substrates and hydrolyze them. This property is known as interfacial activation, which may cause conformational changes on the enzyme to access the hydrophobic substrates (Zhang et al 2019). When mixed bile saltphospholipid micelles are present near the "lid" site, they undergo a conformational change that permits binding of the substrate to the active site of the lipase.…”