A green approach for the biosynthesis of silver nanoparticles (AgNPs) was developed using Diplazium esculentum aqueous extract as green reductant. The bio-stabilized AgNPs were characterized by UV-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS), and high-resolution transmission electron microscopy (HRTEM). The formation of AgNPs was indicated by the colour change and the absorption peak at 449 nm in the UV-Vis spectrum. FTIR spectral analysis suggested the possible biomolecules associated with the reduction of silver ions. The crystalline and face-centered cubic (fcc) structure of AgNPs was obtained by the XRD study. HRTEM analysis showed that the biosynthesized AgNPs composed of quasi-spherical, hexagonal, and ellipsoidal shapes with an average particle size of 23.385 ± 8.349 nm. The biosynthesized AgNPs exhibited good catalytic activity up to 91% reduction of Methylene blue (MB), which followed a reductive pathway rather than a photocatalytic route. The catalytic reduction follows a pseudo-first-order reaction with a rate constant of 0.1051 min -1 . The disc diffusion technique (DDT) and minimal inhibitory concentration (MIC) results showed significant antibacterial capability against Gram-positive (Escherichia coli ATCC 11,229) and Gram-negative (Staphylococcus aureus ATCC 6538) bacteria.