Catalytic Hydrogen Evolution at Mercury Electrodes from Solutions of Peptides and Proteins

Heyrovský, Michael

doi:10.1016/s1871-0069(05)01018-9

Cited by 22 publications

(5 citation statements)

References 119 publications

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“…Catalytic HER processes strongly depend on the buffer concentration because availability of proton donors is important for this electrocatalytic process as recently shown in proteins, some peptides, and polysaccharides . We were interested in the effects of buffer concentration on peak

H_{DNA}

and particularly on peak

H_{DNA}^{D}

in ssDNA.…”

Section: Resultssupporting

confidence: 84%

“…Both peaks

H_{DNA}

and

H_{DNA}^{D}

were sensitive to the DNA structure showing higher peaks for ssDNA as compared to dsDNA. The CPS and voltammetric peaks greatly increased with the buffer concentration in agreement with their involvement in HER or DER. In this respect they greatly differed from DNA peaks CA (due to reduction of cytosine and adenine residues), showing only little dependence on the buffer concentration.…”

Section: Discussionmentioning

confidence: 99%

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Catalytic Deuterium Evolution and H/D Exchange in DNA

Dorčák

Paleček

2018

ChemElectroChem

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Recently, it has been shown that DNA catalyzes the hydrogen evolution reaction (HER) at mercury-containing electrodes, which provide voltammetric and chronopotentiometric stripping (CPS) peaks H DNA , allowing label-free DNA determination at very low concentrations. Here, we compared DNA catalytic HER in H 2 O with catalytic deuterium evolution reaction (DER) in D 2 O. We show that in D 2 O single-stranded (ss) and double-stranded (ds) DNAs produced CPS peaks H D DNA due to DER. Compared to peaks H DNA obtained in H 2 O, peaks H D DNA were smaller and appeared at more negative potentials. Under conditions where dsDNA did not produce any peak H D DNA in D 2 O, this DNA produced in H 2 O peak H DNA . These results suggested a lack of accessible bases in the D 2 O surface-attached dsDNA. This might be attributed to the greater stability of deuterated dsDNA, better resisting the electric field effects at the negatively charged interface. The process of protonation of deuterated ssDNA was very fast in contrast to that of dsDNA, which was much slower and could be followed by CPS in a time range of tens of minutes.[a] Dr.

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H_{DNA}

and particularly on peak

H_{DNA}^{D}

in ssDNA.…”

Section: Resultssupporting

confidence: 84%

“…Both peaks

H_{DNA}

and

H_{DNA}^{D}

Section: Discussionmentioning

confidence: 99%

Catalytic Deuterium Evolution and H/D Exchange in DNA

Dorčák

Paleček

2018

ChemElectroChem

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“…5). Higher sensitivity of peak H is due to catalytic nature of the electrode process involving amino acid residues with labilized protons such as lysine, arginine and cysteine [27,42]. In contrast to hundreds of electrons transferred during catalytic process, protein oxidation is limited to 2-electron processes involving tyrosine and tryptophan residues.…”

Section: Determination Of Rbp At Nanomolar and Subnamolar Concentrationsmentioning

confidence: 99%

Electrochemistry of riboflavin-binding protein and its interaction with riboflavin

Bartošík

Ostatná

Paleček

2009

Bioelectrochemistry

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“…Hydrogen is then generated by transferring of the proton from the acidic component of the buffer to the negatively charged surface of the mercury, as was described in [5].…”

mentioning

confidence: 99%

Chronopotentiometric Analysis of Proteins at Charged Electrode Surfaces

et al. 2019

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Protein properties and functions are strongly dependent on the structure and amino acid content. In this work, catalytic hydrogen evolution reaction (CHER) of five proteins (human serum albumin, lysozyme, β‐synuclein, H2 A and H3 histones) were studied using constant current chronopotentiometric stripping (CPS) with the aim to find out the association between protein content and its electrochemical response. We have shown that the height and potential of CPS peak H in dependence on accumulation potential differed for the studied proteins, while the CPS peak area was almost the same for all of them. CV and CPS peaks H of Cys‐containing proteins appeared at less negative potentials in comparison to proteins without Cys, suggesting easier CHER. Acidic and basic proteins not containing Cys can be also recognized due to their different CPS response after their adsorption at the positive and negative charged interface.

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Catalytic Hydrogen Evolution at Mercury Electrodes from Solutions of Peptides and Proteins

Cited by 22 publications

References 119 publications

Catalytic Deuterium Evolution and H/D Exchange in DNA

Catalytic Deuterium Evolution and H/D Exchange in DNA

Electrochemistry of riboflavin-binding protein and its interaction with riboflavin

Chronopotentiometric Analysis of Proteins at Charged Electrode Surfaces

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