Electrochemistry of riboflavin-binding protein and its interaction with riboflavin

Bartošík, Martin; Ostatná, Veronika; Paleček, Emil

doi:10.1016/j.bioelechem.2009.04.006

Cited by 23 publications

(15 citation statements)

References 50 publications

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“…Soon, CPS was shown to be the most sensitive EC label-free method allowing the determination of proteins at nanomolar and subnanomolar concentrations, 155 , 253 suggesting a wide application of peak H in the analysis of peptides and proteins. 105 , 208 , 209 , 230 − 233 , 239 , 254 , 256 , 257…”

Section: Label-free Electrocatalysis In Peptides and Proteinsmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Paleček

Tkáč²,

Bartošík³

et al. 2015

Chem. Rev.

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289

276

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Section: Label-free Electrocatalysis In Peptides and Proteinsmentioning

confidence: 99%

“…This behavior agreed well with changes in peak H of aqueous-soluble riboflavin-binding protein after riboflavin binding. 256 …”

Section: Label-free Protein Analysis In Biomedicinementioning

confidence: 99%

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Paleček

Tkáč²,

Bartošík³

et al. 2015

Chem. Rev.

Self Cite

289

276

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“…RfBP is mainly produced in the blood plasma of podocyte and binds to the plasma membrane via a ligand binding domain located in the N-terminal region of RfBP sequence (Monaco 1997; Latha et al 2008). RfBP also has a C-terminal phosphorylated domain that accommodates the RIB molecule and enables RfBP to bind RIB tightly in a 1:1 molar ratio (Monaco 1997;Kozik 1982;Bartosík et al 2009). Owing to the structural features, RfBP plays a role in the subcellular translocation of RIB in the animals (Huang and Swaan 2000;Foraker et al 2003).…”

Section: Introductionmentioning

confidence: 98%

Down-regulation of free riboflavin content induces hydrogen peroxide and a pathogen defense in Arabidopsis

Deng

Sun

et al. 2011

Plant Mol Biol

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Riboflavin mediates many bioprocesses associated with the generation of hydrogen peroxide (H₂O₂), a cellular signal that regulates defense responses in plants. Although plants can synthesize riboflavin, the levels vary widely in different organs and during different stages of development, indicating that changes in riboflavin levels may have physiological effects. Here, we show that changing riboflavin content affects H₂O₂ accumulation and a pathogen defense in Arabidopsis thaliana. Leaf content of free riboflavin was modulated by ectopic expression of the turtle gene encoding riboflavin-binding protein (RfBP). The RfBP-expressing Arabidopsis thaliana (REAT) plants produced the RfBP protein that possessed riboflavin-binding activity. Compared with the wild-type plant, several tested REAT lines had >70% less flavins of free form. This change accompanied an elevation in the level of H₂O₂ and an enhancement of plant resistance to a bacterial pathogen. All the observed REAT characters were eliminated due to RfBP silencing (RfBPi) under REAT background. When an H₂O₂ scavenger was applied, H₂O₂ level declined in all the plants, and REAT no longer exhibited the phenotype of resistance enhancement. However, treatment with an NADPH oxidase inhibitor diminished H₂O₂ content and pathogen defense in wild-type and RfBPi but not in REAT. Our results suggest that the intrinsic down-regulation of free flavins is responsible for NADPH oxidase-independent H₂O₂ accumulation and the pathogen defense.

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“…Regarding to the RfBP protein, it is mainly produced in the blood plasma of podocyte and localizes to the plasma membrane via the N-terminal ligand-binding domain [23,24]. RfBP also employs the C-terminal phosphorylated domain to tightly bind riboflavin in a 1:1 molar ratio [24–26]. Owing to these features, RfBP functions to mediate the cellular translocation of riboflavin in the animals [27,28].…”

Section: Introductionmentioning

confidence: 99%

Downregulation of leaf flavin content induces early flowering and photoperiod gene expression in Arabidopsis

Zhu

Tian

et al. 2014

BMC Plant Biol

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BackgroundRiboflavin is the precursor of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), essential cofactors for many metabolic enzymes that catalyze a variety of biochemical reactions. Previously we showed that free flavin (riboflavin, FMN, and FAD) concentrations were decreased in leaves of transgenic Arabidopsis plants expressing a turtle riboflavin-binding protein (RfBP). Here, we report that flavin downregulation by RfBP induces the early flowering phenotype and enhances expression of floral promoting photoperiod genes.ResultsEarly flowering was a serendipitous phenomenon and was prudently characterized as a constant phenotype of RfBP-expressing transgenic Arabidopsis plants in both long days and short days. The phenotype was eliminated when leaf free flavins were brought back to the steady-state levels either by the RfBP gene silencing and consequently nullified production of the RfBP protein, or by external riboflavin feeding treatment. RfBP-induced early flowering was correlated with enhanced expression of floral promoting photoperiod genes and the florigen gene FT in leaves but not related to genes assigned to vernalization, autonomous, and gibberellin pathways, which provide flowering regulation mechanisms alternative to the photoperiod. RfBP-induced early flowering was further correlated with increased expression of the FD gene encoding bZIP transcription factor FD essential for flowering time control and the floral meristem identity gene AP1 in the shoot apex. By contrast, the expression of FT and photoperiod genes in leaves and the expression of FD and AP1 in the shoot apex were no longer enhanced when the RfBP gene was silenced, RfBP protein production canceled, and flavin concentrations were elevated to the steady-state levels inside plant leaves.ConclusionsToken together, our results provide circumstantial evidence that downregulation of leaf flavin content by RfBP induces early flowering and coincident enhancements of genes that promote flowering through the photoperiod pathway.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0237-z) contains supplementary material, which is available to authorized users.

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Electrochemistry of riboflavin-binding protein and its interaction with riboflavin

Cited by 23 publications

References 50 publications

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics

Down-regulation of free riboflavin content induces hydrogen peroxide and a pathogen defense in Arabidopsis

Downregulation of leaf flavin content induces early flowering and photoperiod gene expression in Arabidopsis

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