In this work, the degradation and mineralization of ortho-toluidine (OT) that is one of the constituents of petrochemical wastewater was investigated by the TiO 2 /O 3 process. The influence of some operational parameters such as concentration of pollutant (30-90 mg L -1 ), initial pH and amounts of TiO 2 was investigated. A radical mechanism with the formation of an anion radical superoxide radical prior to hydroxyl radical is suggested for describing the interaction between ozone and TiO 2 . These results were not similar to the ozonation process alone, in which higher pH had a positive effect on the removal of OT because of the generation of hydroxyl radicals. In optimum pH for the ozonation and O 3 /TiO 2 processes, the degradation efficiency of the OT was 89.5 and 96%, respectively, at 60 min of reaction. Furthermore, it was made clear that in catalytic ozonation, the degradation efficiency of the OT was higher at neutral pH conditions (pH = 7). The removal of chemical oxygen demand (COD) was increased from 47.5% (only ozonation) to 73% (O 3 /TiO 2 ) after 90 min of reaction. The kinetics of degradation was pseudo-first order; the degradation and relative mineralization of the OT were calculated by HPLC and COD tests, respectively.