Catalytic properties of recombinant Thermomyces lanuginosus lipase immobilized by impregnation into mesoporous silica in the enzymatic esterification of saturated fatty acids with aliphatic alcohols

Коваленко, Г. А.; Perminova, L. V.; Беклемишев, А. Б.

doi:10.1007/s11144-019-01648-z

Cited by 21 publications

(12 citation statements)

References 32 publications

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“…The most pronounced specificity was observed toward the position of OHgroup in alcohol molecules. The rates of esterification of fatty acids with secondary alcohol (sec-propanol and sec-butanol) was two order of magnitude lower than those with primary alcohols, for example, 0.36 U/g vs. 56.1 U/g for sec-and primpropanol, respectively [18]. The esterification of fatty acid with ternary alcohols (tert-butanol) did not occur, and reaction rate was zero.…”

Section: Matrix Of Relative Activities Of the Immobilized Recombinantmentioning

confidence: 90%

“…It was found that immobilized on silica rPichia/lip was not sensitive to isomerism of alcohols' molecule, and the rates of esterification of fatty acids with primary n-C4,C5 alcohols were almost 10 AE 4% lower than those with is0-C4,C5 alcohols [18]. The most pronounced specificity was observed toward the position of OHgroup in alcohol molecules.…”

Section: Matrix Of Relative Activities Of the Immobilized Recombinantmentioning

confidence: 96%

“…The authors and their collaborators have developed and researched systematically the heterogeneous biocatalysts in which the enzymatic active component was a recombinant T. lanuginosus lipase. These lipase-active biocatalysts were prepared both by entrapment of fully disrupted cells (lysates) of recombinant strainproducer rE.coli/lip inside silica xerogel and its nanocarbon-containing composites [13,14,17] and by adsorption of T. lanuginosus lipase produced by recombinant lipase on silica [15][16][17][18] and carbon aerogel [19]. These biocatalysts were studied in the reactions of tributyrin hydrolysis [13,19], interesterification of oil-fat blends and vegetable oil triglycerides with ethyl acetate [13,14], and esterification of fatty acids [15][16][17][18][19].…”

Section: Lipase-active Heterogeneous Biocatalysts For Vegetable Oil Amentioning

confidence: 99%

“…For example, a minimal volume of lipase solution used for spontaneous adsorption on 1 g of silica was 3.0 mL, whereas for forcible adsorption, it was 0.8 mL equal to total pore volume (V Σ ) of SiO 2 . All results described here referred to the lipase-active heterogeneous biocatalysts (designated as LipoSil) prepared by forcible adsorption on silica of recombinant lipase, which were used predominantly in esterification processes [15,16,18].…”

Section: Biocatalysts Prepared By Adsorption Of Recombinant Lipase Onmentioning

confidence: 99%

“…Of particular scientific and practical interest is the research of the possibility of modulating these properties, by engineering heterogeneous biocatalysts, in particular, by selecting the chemical nature of the supports [20,21]. In our research, the specificity of heterogeneous enzymatic esterification was determined by comparing the reaction rates for various pairs of substrates-saturated fatty acids and aliphatic primary n-alcohols-and the matrix of relative units of activities for the LipoSil biocatalyst was composed ( Table 1) [18].…”

Section: Biocatalysts Prepared By Adsorption Of Recombinant Lipase Onmentioning

confidence: 99%

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Heterogeneous Biocatalysts for the Final Stages of Deep Processing of Renewable Resources into Valuable Products

Коваленко¹,

Perminova²

2021

Molecular Biotechnology

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Heterogeneous biocatalysis is a part of biotechnology and it has commercial potential for industrial implementation, in particular the final stages of deep processing of renewable raw materials. The commercially attractive heterogeneous biocatalysts are prepared by immobilizing practically valuable enzymatic active substances onto solid inorganic supports. Heterogeneous biocatalytic processes of the target conversion of substrate into valuable market product are carried out in periodic or continuous modes using traditional batch and packed-bed reactors, as well as novel types of vortex reactors in accordance with the principles of green chemistry. Heterogeneous biocatalysts for the final stages of deep processing of vegetable raw materials such as starch and oils are described here. One of the biocatalysts is glucoamylase immobilized by adsorption on mesoporous carbon support Sibunit™ type. This glucoamylase-active biocatalyst is used at the stage of starch saccharification, i.e., hydrolysis of dextrin to treacle and glucose syrups used in food and confectionary industries. The second of the biocatalysts is recombinant T. lanuginosus lipase immobilized on mesoporous silica KSK™ type and macroporous carbon aerogel. These lipase-active biocatalysts can effectively compete with traditional organic synthesis catalysts, and they are used in low-temperature processes carried out in unconventional anhydrous media such as interesterification of vegetable oils’ triglycerides with ethyl acetate for producing ethyl esters of fatty acids (biodiesel and vitamin F) and esterification of fatty acids with aliphatic alcohols for synthesis of various esters used as fragrances, flavorings, odors, emollients, and nonionic surfactants in perfume and cosmetics industries. The prepared heterogeneous biocatalysts due to their high enzymatic activity and operational stability are promising for practical implementation.

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Section: Matrix Of Relative Activities Of the Immobilized Recombinantmentioning

confidence: 90%

Section: Matrix Of Relative Activities Of the Immobilized Recombinantmentioning

confidence: 96%

Section: Lipase-active Heterogeneous Biocatalysts For Vegetable Oil Amentioning

confidence: 99%

Section: Biocatalysts Prepared By Adsorption Of Recombinant Lipase Onmentioning

confidence: 99%

Section: Biocatalysts Prepared By Adsorption Of Recombinant Lipase Onmentioning

confidence: 99%

See 3 more Smart Citations

Heterogeneous Biocatalysts for the Final Stages of Deep Processing of Renewable Resources into Valuable Products

Коваленко¹,

Perminova²

2021

Molecular Biotechnology

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Chemically Modified Lipase from Thermomyces lanuginosus with Enhanced Esterification and Transesterification Activities

2021

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Lipase from Thermomyces lanuginosus is one of the most explored enzymes for the esterification of several added-value industrial compounds, such as biodiesel, fragrances, and flavors. Its selectivity in these reactions is mostly related with its activity towards small alcohols. In this work, the impact of the chemical modification, with 4 dodecyl chains at its surface, was evaluated regarding its transesterification and esterification activities, comparing with the native form. Linear size-differentiated alcohols (from 1 to 20 carbons in the aliphatic chain) were used to explore for the first time the effect of the chain length in both transesterification and esterification reactions, using p-nitrophenyl palmitate and oleic acid as model compounds, respectively. The chemically modified lipase showed an outstanding improvement of its catalytic performance than the native enzyme, being this increase directly proportional to the size of the alcohols chain used as substrates. The enormous potential and remarkable versatility of this novel super catalyst was here demonstrated, where diverse types of esters, differing in their potential applications (biodiesel, cosmetics, fine chemistry), were efficiently synthesized. The produced esters were fully characterized by 1 H NMR, GC-MS, and FTIR.

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Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate

da Silva,

Rangel,

Rosa

et al. 2023

Bioprocess Biosyst Eng

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Catalytic properties of recombinant Thermomyces lanuginosus lipase immobilized by impregnation into mesoporous silica in the enzymatic esterification of saturated fatty acids with aliphatic alcohols

Cited by 21 publications

References 32 publications

Heterogeneous Biocatalysts for the Final Stages of Deep Processing of Renewable Resources into Valuable Products

Heterogeneous Biocatalysts for the Final Stages of Deep Processing of Renewable Resources into Valuable Products

Chemically Modified Lipase from Thermomyces lanuginosus with Enhanced Esterification and Transesterification Activities

Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate

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