Catalytically Inactive Endoglycosidases as Microbial Diagnostic Reagents: Chitinases and Lysozymes as Fungal and Bacterial Capture/Label Agents

Improving the Detection of Fungi in Eosinophilic Mucin

“…The use of a novel fluorescein‐labeled chitinase stain confirmed this suspicion because it further increased the fungal detection rate as compared with that obtained using GMS stain. On serial sections, chitinase was clearly highlighting fungal organisms that were invisible in the corresponding GMS stain 14 . This suggested for the first time that the GMS method used thus far in all the previous studies lacked sensitivity to detect fungal organisms in eosinophilic mucin 15 …”

“…On serial sections, chitinase was clearly highlighting fungal organisms that were invisible in the corresponding GMS stain. 14 This suggested for the first time that the GMS method used thus far in all the previous studies lacked sensitivity to detect fungal organisms in eosinophilic mucin. 15…”

“…On serial sections, chitinase was clearly highlighting fungal organisms that were invisible in the corresponding GMS stain. 14 This suggested for the first time that the GMS method used thus far in all the previous studies lacked sensitivity to detect fungal organisms in eosinophilic mucin. 15 The GMS stain has been the gold standard in the histological identification of fungi in mucus; however, there has been variable success in identifying fungi using GMS.…”