Improving the Detection of Fungi in Eosinophilic Mucin

Guo, Canting; Ghadersohi, Saied; Kephart, Gail M.; Laine, Roger A.; Sherris, David A.; Kita, H.; Ponikau, Jens U.

doi:10.1177/0194599812451010

Cited by 30 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, eosinophilic mucinous rhinosinusitis as a distinct entity has been called into questioned by those that feel that sensitive methods to evaluate for fungus have not been applied fully to these patients [20,21,26,29]. Guo et al [34] using conventional Gomori methenamine silver stain (GMS) on eosinophilic mucin observed fungal organisms in only 27 % of specimens; however with trypsin pre-digestion followed by GMS, the yield was increased to 91 % of specimens. In addition, using antibodies targeting chitinase and Alternaria sp.…”

Section: Allergic Fungal Rhinosinusitismentioning

confidence: 99%

See 1 more Smart Citation

Pathology of Fungal Rhinosinusitis: A Review

Montone

2016

Head and Neck Pathol

130

126

View full text Add to dashboard Cite

show abstract

Section: Allergic Fungal Rhinosinusitismentioning

confidence: 99%

“…In addition, using antibodies targeting chitinase and Alternaria sp. antigens, fungi have been reported in 90-100 % of eosinophilic mucin samples, even those negative for histochemical stains [34].…”

Section: Allergic Fungal Rhinosinusitismentioning

confidence: 99%

Pathology of Fungal Rhinosinusitis: A Review

Montone

2016

Head and Neck Pathol

130

126

View full text Add to dashboard Cite

show abstract

“…In surgical specimens from healthy controls, the panfungal DNA was positive in 10 of 15 cases but all were negative for the Alternaria DNA. Studies have also shown that pre-digestion of tissue slides with trypsin before staining dramatically improves identification of fungi by immunofluorescence as does as PCR-DNA analysis [30,31]. …”

Section: Chronic Rhinosinusitis (Crs)mentioning

confidence: 99%

“…Surgical debridement is also a common treatment in CRS [1,2,53]. Identifying specific fungal organisms in CRS caused by mold requires specific fungal staining methods to identify hyphae in sinus specimens or identification of mold by Q-PCR [30,31,54,55]. Treatment of fungal CRS may require the use of oral antifungals, as well as intranasal sprays with antifungal activity, depending upon the improvement of individual patient condition [56].…”

Section: Antifungal Therapy Directed At the Sinusesmentioning

confidence: 99%

Chronic Illness Associated with Mold and Mycotoxins: Is Naso-Sinus Fungal Biofilm the Culprit?

Brewer

Thrasher²,

Hooper³

2013

Toxins

View full text Add to dashboard Cite

It has recently been demonstrated that patients who develop chronic illness after prior exposure to water damaged buildings (WDB) and mold have the presence of mycotoxins, which can be detected in the urine. We hypothesized that the mold may be harbored internally and continue to release and/or produce mycotoxins which contribute to ongoing chronic illness. The sinuses are the most likely candidate as a site for the internal mold and mycotoxin production. In this paper, we review the literature supporting this concept.

show abstract

“…A. alternata has been previously implicated in cases of CRS in the United States [20] and it is among the most ubiquitous phyloplane fungal species in Toronto [23,24]. The presence of A. alternata conidia in the upper respiratory tract has been demonstrated in microscopic and immunologic studies of allergic mucin derived from CRS subjects [25-27]. In vitro studies have also shown that A. alternata extracts induce the recruitment of eosinophils, induce interferon-y, IL-5 and IL-13 in peripheral blood mononuclear cells [20,28]; and regulates the expression of surfactant protein D and LL-37 in fungal sensitized CRS patients [29,30].…”

Section: Discussionmentioning

confidence: 99%

Allergic sensitization in Canadian chronic rhinosinusitis patients

Green

Beezhold

Gallinger³

et al. 2014

All Asth Clin Immun

View full text Add to dashboard Cite

BackgroundChronic rhinosinusitis (CRS) is a societal burden and cause of morbidity in Canada; however, the prevalence of allergic sensitization in Canadian CRS patients has remained poorly characterized.ObjectiveIn this study, we used skin prick test (SPT) and specific immunoglobulin E (sIgE) and G (sIgG) titers to regionally relevant allergen sources in order to determine whether allergic sensitization is more prevalent in CRS patients compared to chronic idiopathic urticaria (CIU) control patients.MethodsOne hundred and fifty eight subjects (19–70 years of age) were recruited into the study. 101 subjects had a confirmed diagnostic history of CRS and 57 subjects with a clinical diagnosis of CIU were recruited as controls. Enrolled subjects underwent SPT to a panel of perennial and seasonal allergens and sIgE titers were quantified to selected environmental allergen mixes (grass, mold, and tree species) using Phadia ImmunoCAP. sIgG was additionally quantified to Alternaria alternata, Aspergillus versicolor, Cladosporium herbarum, and Stachybotrys atra. Differences between CRS and control CIU patient SPT and serological data were examined by chi-squared analysis and analysis of variance.ResultsReactivity to at least one SPT extract occurred in 73% of CRS patients. Positive SPT reactivity to A. alternata (odds ratio (OR): 4.34, 95% confidence interval: 1.57, 12.02), cat (OR: 3.23, 95% CI: 1.16, 9.02), and ragweed (OR: 2.31, 95% CI: 1.02, 5.19) extracts were more prevalent in patients with CRS (p < 0.05). Although dust mite and timothy grass sensitization approached statistical significance in the chi-squared analysis of SPT data, other common perennial and seasonal allergens were not associated with CRS. No statistically significant differences were observed between mean sIgE and sIgG titers in CRS and control patients.ConclusionsThis study supports previous data that suggests A. alternata sensitization is associated with CRS; however, these findings additionally highlight the contribution of other regionally important allergens including cat and ragweed.

show abstract

Improving the Detection of Fungi in Eosinophilic Mucin

Cited by 30 publications

References 30 publications

Pathology of Fungal Rhinosinusitis: A Review

Pathology of Fungal Rhinosinusitis: A Review

Chronic Illness Associated with Mold and Mycotoxins: Is Naso-Sinus Fungal Biofilm the Culprit?

Allergic sensitization in Canadian chronic rhinosinusitis patients

Contact Info

Product

Resources

About