Catechin production in cultured cells of Taxus cuspidata and Taxus baccata

Bulgakov, Victor P.; Tchernoded, G. K.; Веселова, М. В.; Fedoreyev, Sergey A.; Музарок, Т. И.; Zhuravlev, Yuri N.

doi:10.1007/s10529-011-0632-6

Cited by 18 publications

(9 citation statements)

References 16 publications

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“…The highest flavonoids content was obtained with 200 μM/LMeJA that which was 1.5 fold that estimated in the wild plants. Similar results ofa 2-fold increase in flavonoids, catechin and epicatechin, of Taxus cuspidate by using MeJA as an elicitor were documented [37]. Also, other increase in phenolic compounds was reported under elicitation with MeJA [38].…”

Section: Rapd Analysissupporting

confidence: 78%

Genetically stable plants with boosted flavonoids content after in vitro regeneration of the endangered Capparis spinosa L

Goda¹,

Sá²,

F³

et al. 2017

Glob Drugs Therap

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Section: Rapd Analysissupporting

confidence: 78%

Genetically stable plants with boosted flavonoids content after in vitro regeneration of the endangered Capparis spinosa L

Goda¹,

Sá²,

F³

et al. 2017

Glob Drugs Therap

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“…Major phenolic compounds of T. cuspidata were summarized in a review work by Wang et al (2010). Bulgakov et al (2011) reported up to a 2-fold increase in catechin and epicatechin flavonoids in cell suspension cultures of T. cuspidata under elicitation with MeJA. In the current study, increased phenolic and flavonoid compounds were observed in cell suspension cultures of T. baccata under MeJA and S elicitations, with the greatest increase observed for the combination of the 2 elicitors (MeJA + S).…”

Section: Discussionmentioning

confidence: 99%

Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

Jalalpour¹,

Shabani²,

Afghani³

et al. 2014

Turk J Biol

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Cell suspension cultures of Taxus baccata were treated with 2 elicitor compounds, methyl jasmonate (MeJA) and squalestatin (S), individually and in combination for 7 days to determine if they mediated the enhancement of biosynthesis of endogenous jasmonate through induction of lipoxygenase (LOX) activity. Total phenolic compounds, total flavonoids, total antioxidants, phenylalanine ammonialyase (PAL), polyphenol oxidase (PPO), and LOX activities in 5-month-old yew cell cultures were studied. Our results showed that MeJA and S could stimulate production of phenol derivatives in cell suspension cultures of T. baccata. In parallel to the induction of phenolic production in elicited cells, results showed that activities of PAL and PPO enzymes and total antioxidants significantly increased in Taxus cells in response to MeJA and S. Maximal activities of lipoxygenase were obtained 48 h after treatment with MeJA (100 µM), S (0.1 µM), and the combination of the 2 elicitors. Results showed that MeJA and S are effective elicitors for increasing phenolic production in Taxus cell suspension cultures, likely through increasing LOX activity followed by an increase in endogenous jasmonate.

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“…In addition to taxoids, flavonoids, phenylpropanoids, and phenolic compounds have been isolated in Taxus species [16, 31, 42, 43]. In our study, the metabolite–metabolite interaction network revealed 222 taxoid-associated metabolites, belonging to 10 major categories.…”

Section: Discussionmentioning

confidence: 67%

Comparative metabolomic analysis reveals the variations in taxoids and flavonoids among three Taxus species

et al. 2019

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BackgroundTrees of the genus Taxus are highly valuable medicinal plants with multiple pharmacological effects on various cancer treatments. Paclitaxel from Taxus trees is an efficient and widely used anticancer drug, however, the accumulation of taxoids and other active ingredients can vary greatly among Taxus species. In our study, the metabolomes of three Taxus species have been investigated.ResultsA total of 2246 metabolites assigned to various primary and secondary metabolic pathways were identified using an untargeted approach. Analysis of differentially accumulated metabolites identified 358 T. media-, 220 T. cuspidata-, and 169 T. mairei-specific accumulated metabolites, respectively. By searching the metabolite pool, 7 MEP pathway precursors, 11 intermediates, side chain products and derivatives of paclitaxel, and paclitaxel itself were detected. Most precursors, initiated intermediates were highly accumulated in T. mairei, and most intermediate products approaching the end point of taxol biosynthesis pathway were primarily accumulated in T. cuspidata and T. media. Our data suggested that there were higher-efficiency pathways to paclitaxel in T. cuspidata and T. media compared with in T. mairei. As an important class of active ingredients in Taxus trees, a majority of flavonoids were predominantly accumulated in T. mairei rather than T. media and T. cuspidata. The variations in several selected taxoids and flavonoids were confirmed using a targeted approach.ConclusionsSystematic correlativity analysis identifies a number of metabolites associated with paclitaxel biosynthesis, suggesting a potential negative correlation between flavonoid metabolism and taxoid accumulation. Investigation of the variations in taxoids and other active ingredients will provide us with a deeper understanding of the interspecific differential accumulation of taxoids and an opportunity to accelerate the highest-yielding species breeding and resource utilization.

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Catechin production in cultured cells of Taxus cuspidata and Taxus baccata

Cited by 18 publications

References 16 publications

Genetically stable plants with boosted flavonoids content after in vitro regeneration of the endangered Capparis spinosa L

Genetically stable plants with boosted flavonoids content after in vitro regeneration of the endangered Capparis spinosa L

Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

Comparative metabolomic analysis reveals the variations in taxoids and flavonoids among three Taxus species

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