(−)-Catechin promotes adipocyte differentiation in human bone marrow mesenchymal stem cells through PPARγ transactivation

Shin, Dong Wook; Kim, Su Nam; Lee, Sang Min; Lee, Woojung; Song, Min Jeong; Park, Sun Mi; Lee, Tae Ryong; Baik, Joo Hyun; Kim, Han Kon; Hong, Jeong Ho; Noh, Minsoo

doi:10.1016/j.bcp.2008.09.033

Cited by 93 publications

(75 citation statements)

References 37 publications

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“…Previous studies reported that human bone marrow derived mesenchymal stem cells (hBM-MSCs), another type of mesenchymal stem cells, are grown and maintained in DMEM containing low glucose (1 g/L) (Chen et al, 2007;Shin et al, 2009). Thus, we performed real-time quantitative RT-PCR to determine if glucose concentration has effects on the gene expression levels of specific markers for hDSPCs under the 3D culture condition.…”

Section: Effects Of Glucose Concentration On the Stemness Of Hdspcsmentioning

confidence: 99%

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Shim

Lee

Shin

2013

Molecules and Cells

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Cell therapy using adult stem cells has emerged as a potentially new approach for the treatment of various diseases. Therefore, it is an essential procedure to maintain the stemness of adult stem cells for clinical treatment. We previously reported that human dermal stem/progenitor cells (hDSPCs) can be enriched using collagen type IV. However, hDSPCs gradually lose their stem cell properties as in vitro passages continue. In the present study, we developed optimized in vitro culture condition to improve the stemness of these hDSPCs. To evaluate whether the stemness of hDSPCs is well sustained in various culture conditions, we measured the expression levels of SOX2, NANOG, and S100B, which are well-known representative dermal progenitor markers. We observed that hDSPCs grown in three-dimensional (3D) culture condition had higher expression levels of those markers compared with hDSPCs grown in two-dimensional (2D) culture condition. Under the 3D culture condition, we further demonstrated that a high glucose (4.5 g/L) concentration enhanced the expression levels of the dermal progenitor markers, whereas O 2 concentration did not affect. We also found that skin-derived precursor (SKP) culture medium was the most effective, among various culture media, in increasing the dermal progenitor marker expression. We finally demonstrated that this optimized culture condition enhanced the expression level of human telomerase reverse transcriptase (hTERT), the proliferation, and the multipotency of hDSPCs, an important characteristic of stem cells. Taken together, these results suggested that this novel in vitro culture condition improves the stemness of hDSPCs.

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Section: Effects Of Glucose Concentration On the Stemness Of Hdspcsmentioning

confidence: 99%

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Shim

Lee

Shin

2013

Molecules and Cells

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“…hBM-MSCs were maintained and differentiated into adipocytes according to the manufacturer's instructions with minor modifications (Janderova et al, 2003;Shin et al, 2009a;Shin et al, 2009b). Briefly, hBM-MSCs were maintained in Dulbecco-modified eagle's medium (DMEM) with low glucose (1 g/L glucose) containing 10% fetal bovine serum (FBS) (Lonza, Walkersville, MD, USA), supplemented with antibiotics and Glutamax TM (Invitrogen, Carlsbad, CA, USA).…”

Section: Cell Culture and Differentiation Of Hbm-mscsmentioning

confidence: 99%

“…In obesity, it has been implicated that hBM-MSCs are recruited to a new adipocyte pool by metabolic changes in vivo (Entenmann and Hauer, 1996;Sakaguchi et al, 2005). The adipocyte differentiation of hBM-MSCs has been exploited to study insulin sensitivity, obesity, and related metabolic diseases in human (Janderova et al, 2003;Shin et al, 2009a;Shin et al, 2009b). In murine pre-adipocytes, the addition of insulin, dexamethasone, and 3-isobutyl-1-methyxanthine (IDX condition) is sufficient to convert virtually all cells into fully differentiated adipocytes (Rosen, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…In murine pre-adipocytes, the addition of insulin, dexamethasone, and 3-isobutyl-1-methyxanthine (IDX condition) is sufficient to convert virtually all cells into fully differentiated adipocytes (Rosen, 2005). In contrast, it has been shown that less than 10% of hBM-MSCs in culture undergo adipocyte differentiation in response to IDX conditions (Entenmann et al, 1996;Janderova et al, 2003;Shin et al, 2009a). For hBM-MSCs, the activation of PPARγ is essential to significantly promote adipocyte differentiation and increase the number of differentiated cells.…”

Section: Introductionmentioning

confidence: 99%

“…For hBM-MSCs, the activation of PPARγ is essential to significantly promote adipocyte differentiation and increase the number of differentiated cells. In this regard, adipocyte differentiation of hBM-MSCs has been used as a cell culture model to evaluate effects of anti-diabetic drugs like PPARγ agonists and sulfonylurea anti-diabetics on insulin sensitivity (Janderova et al, 2003;Shin et al, 2009a).…”

Section: Introductionmentioning

confidence: 99%

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15-Hydroxyprostaglandin Dehydrogenase Is Associated with the Troglitazone-Induced Promotion of Adipocyte Differentiation in Human Bone Marrow Mesenchymal Stem Cells

Noh¹,

Lee²

2010

Biomolecules and Therapeutics

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-Adipocyte differentiation in human bone marrow mesenchymal stem cells (hBM-MSCs) is not as efficient as that in murine pre-adipocytes when induced by adipogenic agents including insulin, dexamethasone, and 3-isobutyl-1-methylxanthine (IDX condition). Therefore, the promotion of adipocyte differentiation in hBM-MSCs has been used as a cell culture model to evaluate insulin sensitivity for anti-diabetic drugs. In hBM-MSCs, PPARγ agonists or sulfonylurea anti-diabetic drugs have been added to IDX conditions to promote adipocyte differentiation. Here we show that troglitazone, a peroxisome proliferator-activated receptor-gamma (PPARγ) agonist, significantly reduced the levels of anti-adipogenic PGE 2 in IDX-conditioned hBM-MSC culture supernatants when compared to PGE2 levels in the absence of PPARγ agonist. However, there was no difference in the mRNA levels of cyclooxygenases (COXs) and the activities of COXs and prostaglandin synthases during adipocyte differentiation in hBM-MSCs with or without troglitazone. In hBM-MSCs, troglitazone significantly increased the mRNA level of 15-hydroxyprostaglandin dehydrogenase (HPGD) which can act to decrease PGE2 levels in culture. These results suggest that the role of PPARγ activation in promoting adipocyte differentiation in hBM-MSCs is to reduce anti-adipogenic PGE 2 levels through the up-regulation of HPGD expression.

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Therapeutic application of nutraceuticals in diabetic nephropathy: Current evidence and future implications

Dehdashtian

Pourhanifeh

Karim

et al. 2020

Diabetes Metabolism Res

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Summary Diabetes mellitus (DM) is a common metabolic disease which may cause several complications, such as diabetic nephropathy (DN). The routine medical treatments used for DM are not effective enough and have many undesirable side effects. Moreover, the global increased prevalence of DM makes researchers try to explore potential complementary or alternative treatments. Nutraceuticals, as natural products with pharmaceutical agents, have a wide range of therapeutic properties in various pathologic conditions such as DN. However, the exact underlying mechanisms have not been fully understood. The purpose of this review is to summarize recent findings on the effect of nutraceuticals on DN.

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(−)-Catechin promotes adipocyte differentiation in human bone marrow mesenchymal stem cells through PPARγ transactivation

Cited by 93 publications

References 37 publications

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

15-Hydroxyprostaglandin Dehydrogenase Is Associated with the Troglitazone-Induced Promotion of Adipocyte Differentiation in Human Bone Marrow Mesenchymal Stem Cells

Therapeutic application of nutraceuticals in diabetic nephropathy: Current evidence and future implications

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