Catechin-Rich Grape Seed Extract Supplementation Attenuates Diet-Induced Obesity in C57BL/6J Mice

Abstract

Background: Grape seed extracts (GSE) are known to present health benefits such as antioxidative and anti-obesity effects in animal models. The purpose of this research is to determine whether the specially manufactured GSE, catechin-rich GSE (CGSE), can protect against obesity induced by a high-fat diet (HFD) and to address the mechanism underlying this effect. Methods: The componential analysis of CGSE was performed using liquid chromatography/mass spectrometry. Oxygen consumption and the respiratory quotient were determined using 500 mg/kg CGSE administered orally for 3 days in 14- to 15-week-old male C57BL/6J mice. Nine-week-old male C57BL/6J mice were supplemented with 0.5 or 1% CGSE in a HFD for 12 weeks, and their body weight and food intake were monitored. Blood and tissue samples were collected and analyzed. Results: The main polyphenol components of CGSE were catechin and epicatechin. CGSE supplementation in the HFD-induced obesity model chronically suppressed the increase in body weight and the weight of fat pads. Furthermore, CGSE improved metabolic parameter abnormalities and upregulated the fatty acid oxidation-related genes in the liver. Conclusions: These findings suggest that CGSE contains monomeric catechins in high concentrations and ameliorates HFD-induced obesity in C57BL/6J mice.

Chardonnay Grape Seed Flour Ameliorates Hepatic Steatosis and Insulin Resistance via Altered Hepatic Gene Expression for Oxidative Stress, Inflammation, and Lipid and Ceramide Synthesis in Diet-Induced Obese Mice

“…Catechin-rich grape seed extract supplementation upregulated expression of genes related to fatty acid oxidation in mouse livers [8]. We also found that a catechin-rich ChrSd diet downregulated genes related to fatty acid synthesis ( Scd1 , Acot11 , Mogat1 ) while upregulating genes related to fatty acid β-oxidation ( Acsl3 ) compared to a control diet.…”

Low doses of grape seed procyanidins reduce adiposity and improve the plasma lipid profile in hamsters

“…However, in most of the experiments performed with animals, the body weight-related effects of polyphenols have been observed after the administration of these compounds for a long time and/or at high doses. [41][42][43][44][45] In this study, we show that the administration of GSPE during a short period of time (15 days) and at low doses, comparable to the average human consumption of procyanidins 32,33 when expressed as metabolic dose, significantly reduces the body weight gain and the adiposity of hamsters fed either an STD or an HFD. Our data demonstrate for the first time that GSPE significantly decreases the weight of all of the white adipose depots studied, both visceral and subcutaneous.…”

Oligomeric Cocoa Procyanidins Possess Enhanced Bioactivity Compared to Monomeric and Polymeric Cocoa Procyanidins for Preventing the Development of Obesity, Insulin Resistance, and Impaired Glucose Tolerance during High-Fat Feeding

“…In the same respect, research specifically evaluating the beneficial attributes of polyphenols derived from materials such as GSE is also extensive. GSE-derived polyphenols have been shown to combat obesity and T2D by alleviating HF DIO, decreasing blood glucose and insulin resistance, and reducing the expression of obesity biomarkers [89][90][91][92][93] . In contrast, studies of the anti-obesity and anti-diabetic effects of cocoa polyphenols are comparatively few.…”