Catecholamine-induced lipolysis in swine

Mersmann, Harry J.; Brown, L. Jackson; Underwood, M.C.; Stanton, H.C.

doi:10.1016/0305-0491(74)90055-8

Cited by 20 publications

(23 citation statements)

References 13 publications

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“…These high concentrations were necessary, since pig adipose tissue seems to have a lower sensitivity to NOR than rat adipose tissue (Mersmann et al 1974;Mersmnn 1984). Moreover, the m Bipolytic response was usually obtained for IW4 M NOR.…”

Section: Dietmentioning

confidence: 97%

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Adipose tissue lipolytic activity and urinary catecholamine excretion in cold-acclimated piglets

Herpin¹,

Bertin²,

Marco³

et al. 1991

Can. J. Physiol. Pharmacol.

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This study was designed to investigate the effect of chronic cold exposure (12 degrees C for 3 weeks) on catecholamine production and noradrenaline-induced lipolytic rate to further describe thermoregulatory mechanisms in 5- to 8-week-old pigs. Lipolytic activity in white adipose tissue was assessed in vitro while catecholamine production was estimated by measuring noradrenaline, adrenaline, and dopamine levels in 24-h urine samples. Animals were fed ad libitum and food intake was 20% greater in the cold. In control piglets maintained in a 23 degrees C environment, the addition of increasing amounts of noradrenaline (10(-6), 10(-5), 10(-4) M) stimulates lipolysis (p less than 0.05) and enhances the basal lipolytic rate (5.4 mu equiv. fatty acids.120 min-1.g-1 tissue) by 2.5-, 2.7-, and 3.9-fold, respectively. Three weeks of cold acclimation had no effect on basal lipolytic rate but increased significantly noradrenaline responsiveness: incubation of subcutaneous white fat in the presence of 10(-4) M noradrenaline does increase the basal lipolytic rate by sixfold. Noradrenaline effects were maximally activated by theophylline. Daily dopamine and noradrenaline excretions (3-10 micrograms/24 h) were increased significantly (up to eightfold) after 10 days of cold acclimation. By contrast, adrenaline excretion was quite low (0.6-1.6 micrograms/24 h) and showed no significant variation with time. It is likely that these hormonal and biochemical modifications play a prominent part in the mechanism of cold acclimation in the piglet. Their significance in the development of shivering and nonshivering thermogenesis is discussed in relation to the supply of energy substrates to the fatty acid utilizing tissues and to the possible uncoupling effect of free fatty acids.

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Section: Dietmentioning

confidence: 97%

“…In vitro Hipolyta'c hsctivi~ detemiwtion LipIytic rate was determined on minced adipose tissue according to a modification of the method described by Mersmann et al (1974). Imediatdy post-mortem, adipose tissue was placed in 0.9% NaCl maintained at 37OC.…”

Section: Dietmentioning

confidence: 99%

Adipose tissue lipolytic activity and urinary catecholamine excretion in cold-acclimated piglets

Herpin¹,

Bertin²,

Marco³

et al. 1991

Can. J. Physiol. Pharmacol.

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“…At the termination o f incubation, the medium was analyzed for fatty acids (FA) by the method o f Kelley (1965) and for glycerol (G) by the method o f Ko and Royer (1968). Other conditions and optimization o f the system for swine adipose tissue were described (Mersmann et at, 1974).…”

Section: Methodsmentioning

confidence: 99%

“…Quantitation o f the dose-related response to various agonists was obtained by leastsquares fitting o f the data for each response variable (fatty acid or glycerol release) and sample to a dose-response curve (probit X log dose) as previously indicated (Mersmann et at, 1974). This resulted in estimates o f the basal response (activity in absence o f exogenous agonist), the maximal attainable lipolytic response (M A X ), and the E D S0 (the amount o f agonist yielding 50 % o f the maximal response) o f the dose-response curve.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies from this laboratory established conditions for measuring lipolysis in swine adipose tissue slices and quantitated the response to catechol amines (Mersmann et a l, 1974). The epinephrine-induced maximal lipolytic response abruptly increased between birth and 7 days post-partum, remained elevated during the suckling period, and decreased somewhat at day 45 and more so at days 70 to 160.…”

Section: Introductionmentioning

confidence: 99%

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Factors Influencing the Lipolytic Response of Swine (<i>Sus domesticus</i>) Adipose Tissue: Ontogeny

Mersmann¹,

Phinney²,

Brown³

1976

Neonatology

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Swine adipose tissue was not responsive to glucagon at any age and only marginally to adrenocorticotropin (ACTH). The latter response could be greatly magnified and the sensitivity increased by addition of theophylline (Theo). The maximal lipolytic activity (MAX) obtained with ACTH plus Theo was minimal in the newborn, greatly increased in young animals, and reduced during the growing period; however, the sensitivity did not change. The MAX for Theo (plus low epinephrine) yielded a pattern similar to ACTH, but the sensitivity changed (most sensitive in younger animals). The MAX and sensitivity obtained with dibutyryl cyclic adenosine monophosphate were not appreciably changed during development.

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Specificity of β-adrenergic control of lipolysis in swine adipose tissue

Mersmann

1984

Comparative Biochemistry and Physiology Part C: Comparative Pha

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Catecholamine-induced lipolysis in swine

Cited by 20 publications

References 13 publications

Adipose tissue lipolytic activity and urinary catecholamine excretion in cold-acclimated piglets

Adipose tissue lipolytic activity and urinary catecholamine excretion in cold-acclimated piglets

Factors Influencing the Lipolytic Response of Swine (<i>Sus domesticus</i>) Adipose Tissue: Ontogeny

Specificity of β-adrenergic control of lipolysis in swine adipose tissue

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