Catecholamine Stress Hormones Regulate Cellular Iron Homeostasis by a Posttranscriptional Mechanism Mediated by Iron Regulatory Protein

Tapryal, Nisha; G, Vishnu Vivek; Mukhopadhyay, Chinmay K.

doi:10.1074/jbc.m114.592519

Cited by 22 publications

(17 citation statements)

References 47 publications

(71 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pcDNA3 plasmid construct containing Fpn 5′UTR was linearised and transcribed using in vitro transcription kit (Roche). Using 32 P–UTP labelled Fpn 5′UTR, RNA gel shift assays were performed following protocols as described before (Biswas et al, ; Tapryal et al, ). For the binding assays, 10 μg of cytoplasmic extracts were incubated with radiolabeled 5′UTR‐IRE probe.…”

Section: Methodsmentioning

confidence: 99%

“…Cytosolic aconitase assay was performed as described previously (Das et al, 2009;Tapryal, Vivek, & Mukhopadhyay, 2015). Cytosolic extracts (50 μg) from uninfected and infected splenocytes were added to 0.2 mM cis-aconitate in 100 mM Tris-Cl, pH 7.4, 100 mM NaCl, BSA (0.02%) to perform aconitase assay as disappearence of cis-aconitate was followed at 25°C at 240 nm.…”

Section: Cytosolic Aconitase Assaymentioning

confidence: 99%

See 1 more Smart Citation

Leishmania donovaniinhibits ferroportin translation by modulating FBXL5-IRP2 axis for its growth within host macrophages

Das

Sandhya

Vivek

et al. 2018

Cellular Microbiology

Self Cite

View full text Add to dashboard Cite

Hepcidin mediated ferroportin (Fpn) degradation in macrophages is a well adopted strategy to limit iron availability towards invading pathogens. Leishmania donovani (LD), a protozoan parasite, resides within macrophage and competes with host for availing iron. Using in vitro and in vivo model of infection, we reveal that LD decreases Fpn abundance in host macrophages by hepcidin independent mechanism. Unaffected level of Fpn-FLAG in LD infected J774 macrophage confirms that Fpn down-regulation is not due its degradation. While increased Fpn mRNA but decreased protein expression in macrophages suggests blocking of Fpn translation by LD infection that is confirmed by S-methionine labelling assay. We further reveal that LD blocks Fpn translation by induced binding of iron regulatory proteins (IRPs) to the iron responsive element present in its 5'UTR. Supershift analysis provides evidence of involvement of IRP2 particularly during in vivo infection. Accordingly, a significant increase in IRP2 protein expression with simultaneous decrease in its stability regulator F-box and leucine-rich repeat Protein 5 (FBXL5) is detected in splenocytes of LD-infected mice. Increased intracellular growth due to compromised expressions of Fpn and FBXL5 by specific siRNAs reveals that LD uses a novel strategy of manipulating IRP2-FBXL5 axis to inhibit host Fpn expression.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Cytosolic Aconitase Assaymentioning

confidence: 99%

Leishmania donovaniinhibits ferroportin translation by modulating FBXL5-IRP2 axis for its growth within host macrophages

Das

Sandhya

Vivek

et al. 2018

Cellular Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Catecholamines effectively cause the release of free-iron from hepatocytes and myocytes such that serum iron levels are increased rapidly. 41 Although this response is likely adaptive (ie, increasing nutrient availability during stress), the increase in background iron is then exacerbated by the iron release that is caused by tissue injury from noninfectious or infectious insult. This rapid double-hit creates iron levels that exceed the binding capacity of TF leading to increased CFI levels thereby increasing ROS.…”

Section: Catalytic Free Iron In Acute Illnessmentioning

confidence: 99%

“…94 The increased CFI environment seen in patients with trauma receiving massive blood transfusion is exacerbated because many trauma patients require catecholamine infusions (which increase serum iron) and suffer severe anemia from hemorrhage, which leads to the release of erythropoietin, which suppresses hepcidin generation thereby increasing CFI. 13,26,41,95 As a consequence, severe trauma is often exacerbated by high levels of CFI. Thus, patients with trauma requiring massive blood transfusion leading to high-levels of CFI may benefit from hepcidin-induced hypoferremia.…”

Section: Trauma and Transfusionmentioning

confidence: 99%

Therapeutic Opportunities for Hepcidin in Acute Care Medicine

Chawla

Beers-Mulroy

Tidmarsh

2019

Critical Care Clinics

View full text Add to dashboard Cite

show abstract

“…It is reported that catecholamines can also modulate ovarian steroidogenic activity in catfish, Heteropneustes fossilis (Joy et al, 2014). Catecholamines stress hormones like epinephrine and norepinephrine regulate energy homeostasis by regulating iron homeostasis in cells (Tapryal et al, 2015). Excess aldosterone is reported to result in decreased glucose uptake and oxidation in skeletal muscle in adult male rat, thus increasing the incidence of type2 diabetes (Selvaraj et al, 2013).…”

Section: Pharmacological Studies On Adrenal Glandmentioning

confidence: 99%

Endocrine Pharmacology Research in India: Scientific Progress in Last Five Years

Srinivasan¹,

Sharma²

2018

Proceedings of the Indian National Science Academy

View full text Add to dashboard Cite

During the last five years, significant scientific effort has been made in the field of endocrine pharmacology by Indian researchers. Endocrine disorders like diabetes and disorders of the reproductive systems and melatonin are the major areas of research interest of the Indian scientists. Though the researchers have investigated both natural products and synthetic compounds, the reported studies have focused primarily on plant extracts to substantiate their use scientifically based on their folklore use. Diabetes is fast gaining the status of a potential epidemic in India with approximately 69 million people in the world. Various pharmacological investigations on herbal and synthetic compounds have been carried out for the treatment of diabetes and its complications. Saroglitazar, a novel PPARα/γ agonist is the first indigenously developed synthetic antidiabetic drug by an Indian pharmaceutical company as approved for the treatment of type 2 diabetes in June 2013. The potential of certain cell based therapies and nanoformulations were also investigated for different diabetes treatment. The scientific rationale for the use of various herbs on male and female reproduction with respect to aphrodiasic, fertility and antifertility properties has been explored. In addition to role of melatonin in chronobiology, it has been reported to possess multiple pharmacological actions such as antioxidant, anti-inflammatory and neuroprotective actions. Only few Indian scientists have studied on endocrine pharmacology related to adrenal, tyroid and parathyroid gland disorders. In addition, the effects of some endocrine disruptors, toxic chemicals and heavy metals on endocrine systems were also studied. In this review, we have compiled the research articles on scientific progress made in the field of endocrine pharmacology from India between years 2012-2017.

show abstract

Catecholamine Stress Hormones Regulate Cellular Iron Homeostasis by a Posttranscriptional Mechanism Mediated by Iron Regulatory Protein

Cited by 22 publications

References 47 publications

Leishmania donovaniinhibits ferroportin translation by modulating FBXL5-IRP2 axis for its growth within host macrophages

Leishmania donovaniinhibits ferroportin translation by modulating FBXL5-IRP2 axis for its growth within host macrophages

Therapeutic Opportunities for Hepcidin in Acute Care Medicine

Endocrine Pharmacology Research in India: Scientific Progress in Last Five Years

Contact Info

Product

Resources

About