Cathepsin D. Characteristics of immunoinhibition and the confirmation of a role in cartilage breakdown

Dingle, J. T.; Barrett, Alan J.; Weston, P.D.

doi:10.1042/bj1230001

Cited by 173 publications

(75 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The effect of enzymes on articular cartilage has been widely studied. Neutral protease (3)(4)(5)(6)(7), acid protease (4,6,(8)(9)(10), lysozyme (1 l), and collagenase (12-14) have been implicated in the erosive process. Acid protease (cathepsin D) (15) has been shown to be present in increased amounts in rheumatoid articular cartilage.…”

mentioning

confidence: 99%

Surface Ultrastructure of Rheumatoid Articular Cartilage

Kimura

Tateishi

Ziff

1977

Arthritis & Rheumatism

View full text Add to dashboard Cite

Six rheumatoid articular cartilage specimens, which appeared grossly normal and were shown to be free of pannus when examined under the light microscope, were examined electron microscopically. For comparison, normal‐appearing cartilage specimens from 2 patients with meniscus injury and 2 with degenerative joint disease (DJD) were also examined. In all cases the normal‐appearing joint surface of rheumatoid cartilage was abnormal. Amorphous‐appearing material was present to a depth varying between 6 and 25 m̈m. Some of this material had the appearance of fibrin deposited at the cartilage surface, but much appeared to represent breakdown products of the cartilage matrix, i.e. degraded collagen and proteoglycan. DJD cartilage did not show similar changes. The findings suggest that the surface of rheumatoid articular cartilage, even when grossly normal in appearance, is degraded by enzymes either present in the synovial fluid or released by polymorphonuclear cells in close contact with the cartilage surface.

show abstract

mentioning

confidence: 99%

Surface Ultrastructure of Rheumatoid Articular Cartilage

Kimura

Tateishi

Ziff

1977

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…On the other hand, lysosomal acid protease is involved in the intra-cellular protein metabolism18). DINGLE et al [19][20][21] and MORRISON et al22) suggested that lysosomal acid protease plays a major role in the metabolism of the cartilage matrix (extracellular protein metabolism). In this study, no significant changes were observed in both enzyme activities (units/g of follicular wall) until ovulation.…”

Section: Discussionmentioning

confidence: 99%

Acid and Neutral Proteases in the Ovarian Follicular Walls in the Domestic Fowl (Gallus domesticus)

Fujii¹,

Tojo²,

Koga³

et al. 1981

Nihon Chikusan Gakkaiho

View full text Add to dashboard Cite

Acid and neutral proteases were found in the ovarian follicular walls in the domestic fowl, and their properties were investigated.Acid protease was present mostly in the soluble fraction of the tissue homogenate in 0.01M CaCl2 containing 0.25% Triton X-100, whereas neutral protease was present mostly in the insoluble fraction. The pH optima for acid and neutral proteases were 4.0 and 7.0, respectively. Both of the captoethanol, EDTA and Ca2+. Both of the enzyme activities were affected by neither chicken serum nor soybean trypsin inhibitor.The acid protease activity (units/g of follicular wall) was significantly increased after ovulation, whereas the neutral protease activity was decreased after ovulation, followed by a subsequent increase many hours later. Jpn. J.Zootech. Sci., 52 (10) : [730][731][732][733][734][735] 1981 The presence of proteolytic enzymes such as acid and neutral proteases in the ovarian follicles has been reported in mammalian1-6) and avian species7). The enzymes are suggested to play an important role in rupture of follicles at ovulation. NAKAMURA and NAKAJO7) studied neutral protease in ovarian follicles in Japanese quails in relation to ovulation. However, little information is available on the properties and physiological roles of the proteolytic enzymes in the ovarian follicles in avian species.The present paper describes the detection and properties of acid and neutral proteases in the ovarian follicles in the domestic fowl. Changes in the activities of these enzymes, before and after ovulation, were also investigated. Materials and MethodsPreparation of ovarian follicular walls. The largest follicles and the post-ovulatory follicles (the most recently ruptured follicles) were obtained from White Leghorn laying hens killed at various hours before and after the expected ovulation of the second ovum of a 5-6 egg clutch. After each follicle was frozen on dry ice, the follicular wall was separated from the yolk Preparation of crude protease.

show abstract

“…Cathepsin D (EC 3.4.23.5) activity was assayed spectrometrically as described previously [22] in incubation mixtures containing buffered haemoglobin solution [4% (w/v) in 0.25M-sodium acetate buffer, pH5.0].…”

Section: Methodsmentioning

confidence: 99%