1972
DOI: 10.1111/j.1365-2621.1972.tb02716.x
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Catheptic Activity of Fish Muscle

Abstract: Catheptic activity was found to be localized in the lysosomal fraction of skeletal muscle of winter flounder (Pseudopleuronectes americanus). The enzyme hydrolyzed hemoglobin, globin, bovine serum albumin and endogenous proteins. The hemoglobin splitting activity of the enzyme under different conditions was determined by gel electrophoresis. An optimum pH of 4.0 was found for both the hemoglobin breakdown and autolytic activity on the endogenous proteins. The enzyme was highly thermostable, dialysis had no eff… Show more

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Cited by 55 publications
(24 citation statements)
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“…Some of these enzymes may indeed show significant heat resistance (Reddi et al . ; Tzong‐Shin and Lanier ) and may explain the gradual proteolytic spoilage of the analyzed trout fillets in the absence of significant microbial growth. In other food products, like sterilized or ultra high temperature milk, it has been reported that heat‐resistant proteases and lipases produced by psychrotrophic microflora during chilled storage could easily survive treatments above 100C – up 149C for 10 s (Adams et al .…”
Section: Resultsmentioning
confidence: 97%
“…Some of these enzymes may indeed show significant heat resistance (Reddi et al . ; Tzong‐Shin and Lanier ) and may explain the gradual proteolytic spoilage of the analyzed trout fillets in the absence of significant microbial growth. In other food products, like sterilized or ultra high temperature milk, it has been reported that heat‐resistant proteases and lipases produced by psychrotrophic microflora during chilled storage could easily survive treatments above 100C – up 149C for 10 s (Adams et al .…”
Section: Resultsmentioning
confidence: 97%
“…1996). It is reasonable to hypothesize that higher NaCl content coupled with lower moisture content in dry‐salted fillets can inhibit the proteolitic activity of endogenous enzymes: it is well known that enzymatic activity is influenced by the water activity of the means (Reddi et al . 1972), which decreases at higher solute concentration.…”
Section: Resultsmentioning
confidence: 99%
“…These conditions facilitate spontaneous extraction of enzymes from the fish to the marinating brine. Results reported by other authors suggest that NaCl concentration in the marinating brine inhibits the activity of aspartyl proteases, including cathepsin D (Gomez-Guillen & Batista, 1997;Reddi, Constantinides, & Dymsza, 1972;Siebert, 1973).…”
Section: Introductionmentioning
confidence: 87%