No abstract

Catheptic activity was found to be localized in the lysosomal fraction of skeletal muscle of winter flounder (Pseudopleuronectes americanus). The enzyme hydrolyzed hemoglobin, globin, bovine serum albumin and endogenous proteins. The hemoglobin splitting activity of the enzyme under different conditions was determined by gel electrophoresis. An optimum pH of 4.0 was found for both the hemoglobin breakdown and autolytic activity on the endogenous proteins. The enzyme was highly thermostable, dialysis had no effect on its activity, and the enzyme was inhibited by sodium chloride, cysteine, ATP, NAD and phenyl methyl sulfonyl fluoride. Its molecular weight was found to be about 32,000 using sucrose gradient centrifugation.

No abstract

Newborn rats, fed either cow's milk or rat's milk by tube, developed a "bloat" and the time required for emptying the stomach increased. When these animals were allowed to suckle on nonlactating foster mothers, no evidence of this syndrome was observed. Based on these observations, a technique was developed for the artificial feeding of newborn rats. When the diet was rat's milk, growth equivalent to that of animals fed solely by lactating females was obtained. A diet of cow's milk did not support adequate growth.

Fresh washed red hake (Urophycis chuss) mince without cryoprotectants was irradiated at 0 (control), 0.66 and 1.31 kGy and stored aerobically at 3.3"C. The total aerobic plate counts of the control and the low and high levels irradiated samples remained less than 10" CFU/ g for 4, 10, and 17 days, respectively. Gel strength decreased after irradiation of mince, and such decreases were dose-dependent. Irradiation extended sensory shelf life of unfrozen fish mince 12-18 days and microbiologically (< 10" CFU/g) 6-13 days longer than the unirradiated control.