Cationic liposome-encapsulated carotenoids as a potential treatment for fibromyalgia in an animal model

Elkholy, Nourhan S; Shafaa, Medhat W.; Mohammed, Haitham S.

doi:10.1016/j.bbadis.2021.166150

Cited by 16 publications

(17 citation statements)

References 42 publications

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“…S2. Consistent with the above results, the content of MDA in βC and VE encapsulated liposomes was remarkably less than that in unloaded liposome (6.44 ± 0.09 nmol mL −1 ), indicating higher LPO‐inhibiting activities 51 . Co‐encapsulated liposomes showed a stronger LPO inhibition capability than L‐βC.…”

Section: Resultssupporting

confidence: 84%

“…Consistent with the above results, the content of MDA in ⊎C and VE encapsulated liposomes was remarkably less than that in unloaded liposome (6.44 ± 0.09 nmol mL −1 ), indicating higher LPO-inhibiting activities. 51 Co-encapsulated liposomes showed a stronger LPO inhibition capability than L-⊎C. In particular, L-VE-⊎C at 5:3 and 3:1 significantly reduced the production of MDA, with a concentration of low to 0.80 ± 0.09 nmol mL −1better than single encapsulated liposomes.…”

Section: Lpo Assaymentioning

confidence: 86%

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Physicochemical properties, antioxidant activities and in vitro sustained release behaviour of co‐encapsulated liposomes as vehicle for vitamin E and β‐carotene

Zhang

Cheng

et al. 2022

J Sci Food Agric

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BACKGROUND In this study the potential of liposomes as a vitamin E (VE) and β‐carotene (βC) delivery system was examined. The co‐encapsulated liposomes of βC and VE (L‐VE‐βC) were prepared and characterized. Their antioxidant properties were evaluated by free radical scavenging activities of 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS), hydroxyl radical and lipid peroxidation assay. The in vitro sustained release behaviour was then investigated and discussed. RESULTS VE and βC were co‐encapsulated in liposomes with high encapsulation efficiency, up to 92.49% and 86.16% for βC and VE, respectively. The antioxidant activities of L‐VE‐βC samples were significantly higher than that of single loaded liposome. Among different ratios of VE/βC, L‐VE‐βC at 5:3 exhibited the highest radical scavenging rates, with 66.80%, 56.58% and 34.39% for DPPH, ABTS and OH radical, respectively. L‐VE‐βC samples also had a good ability to inhibit lipid peroxidation, especially the sample with ratios of VE/βC at 5:3 and 3:1. In simulated gastrointestinal release, L‐VE‐βC exhibited an excellent sustained release behaviour in SGF with the accumulated rate at about 20%, while the release rate in SIF increased to over 80%, where they should be absorbed. The release kinetics analysis indicated that βC was released in the Higuchi model in stomach, and the Korsmeyr–Peppas model in intestine. CONCLUSION Compared to single loaded liposomes, the combined‐loaded liposomes exhibited higher antioxidant activity and bioavailability, suggesting the potential applications in functional foods. © 2022 Society of Chemical Industry.

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Section: Resultssupporting

confidence: 84%

Section: Lpo Assaymentioning

confidence: 86%

Physicochemical properties, antioxidant activities and in vitro sustained release behaviour of co‐encapsulated liposomes as vehicle for vitamin E and β‐carotene

Zhang

Cheng

et al. 2022

J Sci Food Agric

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“…Nowadays, various methods are proposed to deliver carotenoids into the cytoplasmic membrane of the cells [ 71 , 72 , 73 , 74 , 75 ]. Yet, there is a lack of quantitative methods to evaluate the efficiency of the carotenoid delivery into the membrane.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Membrane Microviscosity by Protein-Mediated Carotenoid Delivery as Revealed by Time-Resolved Fluorescence Anisotropy

et al. 2022

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Carotenoids are potent antioxidants with a wide range of biomedical applications. However, their delivery into human cells is challenging and relatively inefficient. While the use of natural water-soluble carotenoproteins capable to reversibly bind carotenoids and transfer them into membranes is promising, the quantitative estimation of the delivery remains unclear. In the present work, we studied echinenone (ECN) delivery by cyanobacterial carotenoprotein AnaCTDH (C-terminal domain homolog of the Orange Carotenoid Protein from Anabaena), into liposome membranes labelled with BODIPY fluorescent probe. We observed that addition of AnaCTDH-ECN to liposomes led to the significant changes in the fast-kinetic component of the fluorescence decay curve, pointing on the dipole-dipole interactions between the probe and ECN within the membrane. It may serve as an indirect evidence of ECN delivery into membrane. To study the delivery in detail, we carried out molecular dynamics modeling of the localization of ECN within the lipid bilayer and calculate its orientation factor. Next, we exploited FRET to assess concentration of ECN delivered by AnaCTDH. Finally, we used time-resolved fluorescence anisotropy to assess changes in microviscosity of liposomal membranes. Incorporation of liposomes with β-carotene increased membrane microviscosity while the effect of astaxanthin and its mono- and diester forms was less pronounced. At temperatures below 30 °C addition of AnaCTDH-ECN increased membrane microviscosity in a concentration-dependent manner, supporting the protein-mediated carotenoid delivery mechanism. Combining all data, we propose FRET-based analysis and assessment of membrane microviscosity as potent approaches to characterize the efficiency of carotenoids delivery into membranes.

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“…The cationic liposomeencapsulated carotenoids (lutein or betacarotene) on a nanosized scale could be a good alternative to the pharmacological interventions for FM treatment. 80 Lutein inhibits IL-1-induced osteoclast differentiation and bone resorption, and lutein also inhibited the survival of mature osteoclasts in culture. When added to osteoblast culture, lutein enhances the formation of mineralized bone nodules by increasing BMP2 expression and inhibiting sclerotin expression.…”

Section: Bioactivity Of Luteinmentioning

confidence: 99%

“…Fibromyalgia (FM) is a chronic pain syndrome characterized by widespread pain in muscles and joints and chronic fatigue. The cationic liposome-encapsulated carotenoids (lutein or betacarotene) on a nanosized scale could be a good alternative to the pharmacological interventions for FM treatment . Lutein inhibits IL-1-induced osteoclast differentiation and bone resorption, and lutein also inhibited the survival of mature osteoclasts in culture.…”

Section: Bioactivity Of Luteinmentioning

confidence: 99%

Advances in Research on Natural Distribution, Biosynthesis, Detection, Bioactivity, and Application of Lutein

Zhao

Ci²,

Pang

et al. 2022

ACS Agric. Sci. Technol.

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Lutein, [(3R,3′R,6′R)-β,ε-carotene-3,3′-diol], is a C40 hydroxyl carotenoid. Its chemical structure is based on an unsaturated polyene chain skeleton, and there are one ε-ring and one β-ring on both sides of the skeleton, with a hydroxyl group on the 3′ site of each ring. Lutein is one of the important lipophilic isoprenes from mainly plants and algae. The wide range of sources makes the study of lutein quite comprehensive. The biosynthetic pathway and biochemical activities of lutein have been extensively studied and revealed. Lutein has been extensively studied for its important bioactivity, including for use in functional foods and medicines for brain health, eye-related diseases, skin health, and cancers. This review of lutein summarizes the major studies on bioactivity, metabolism, stability, digestive absorption, lutein delivery systems, and biotechnology.

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Cationic liposome-encapsulated carotenoids as a potential treatment for fibromyalgia in an animal model

Cited by 16 publications

References 42 publications

Physicochemical properties, antioxidant activities and in vitro sustained release behaviour of co‐encapsulated liposomes as vehicle for vitamin E and β‐carotene

Physicochemical properties, antioxidant activities and in vitro sustained release behaviour of co‐encapsulated liposomes as vehicle for vitamin E and β‐carotene

Modulation of Membrane Microviscosity by Protein-Mediated Carotenoid Delivery as Revealed by Time-Resolved Fluorescence Anisotropy

Advances in Research on Natural Distribution, Biosynthesis, Detection, Bioactivity, and Application of Lutein

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