2012
DOI: 10.1016/j.anireprosci.2012.02.004
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Cattle fetal sex determination by polymerase chain reaction using DNA isolated from maternal plasma

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Cited by 17 publications
(10 citation statements)
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“…Serum from 14 rhinoceroses pregnant with 7 male and 7 female fetuses during 61–490 days of gestation were analyzed by PCR‐based sexing, and corroborated the sex of each delivered calf following a reamplification step for SRY . Similarly high levels of accuracy were documented for other PCR‐based assays in maternal serum from bovine (da Cruz et al, ; Davoudi et al, ; Lemos et al, ; Wang et al, ), ovine (Kadivar et al, ; Saberivand & Ahsan, ), and equine (de Leon et al, ; Kadivar et al, ). The earliest that fetal sex determination was made was 61 (female fetus) and 72 (male fetus) days of gestation, which is the timeframe when fetal sex can be determined via rectal ultrasound in all rhinoceros species (Radcliffe et al, , ; Roth et al, ; Stoops et al, ).…”
Section: Discussionsupporting
confidence: 54%
See 1 more Smart Citation
“…Serum from 14 rhinoceroses pregnant with 7 male and 7 female fetuses during 61–490 days of gestation were analyzed by PCR‐based sexing, and corroborated the sex of each delivered calf following a reamplification step for SRY . Similarly high levels of accuracy were documented for other PCR‐based assays in maternal serum from bovine (da Cruz et al, ; Davoudi et al, ; Lemos et al, ; Wang et al, ), ovine (Kadivar et al, ; Saberivand & Ahsan, ), and equine (de Leon et al, ; Kadivar et al, ). The earliest that fetal sex determination was made was 61 (female fetus) and 72 (male fetus) days of gestation, which is the timeframe when fetal sex can be determined via rectal ultrasound in all rhinoceros species (Radcliffe et al, , ; Roth et al, ; Stoops et al, ).…”
Section: Discussionsupporting
confidence: 54%
“…Determining fetal sex using sex‐specific DNA sequences in maternal serum by PCR is successfully performed in species with varied types of placentation and hence different degrees of maternal fetal contact—including horses (de Leon et al, ; Kadivar et al, ), cattle (da Cruz et al, ; Davoudi et al, ; Lemos et al, ; Wang et al, ), sheep (Asadpour, Asadi, Jafari‐Joozani, & Hamidian, ; Kadivar et al, ), humans (Lo et al, ), and primates (Jimenez & Tarantal, ; Yasmin, Takano, Nagai, Otsuki, & Sandai, ). SRY (Sex‐determining region Y) is the most commonly used gene for sex typing in mammals, particularly when an internal control is included to detect a gene present on the X chromosome as validation of the amplification process.…”
Section: Introductionmentioning
confidence: 99%
“…The genetic diversity of the Y chromosome was determined as lower than that of autosomal chromosomes (Liu et al, 2003;Hellborg and Ellegren, 2004;Ginja et al, 2009). For prenatal sex determination in cattle, fetal sexes were successful determined at 99.9% after 55 days of pregnancy using circulating fetal DNA (da Cruz et al, 2012).…”
Section: Introductionmentioning
confidence: 99%
“…() and da Cruz et al. (), where a similar type of DNA extraction method showed satisfactory test results (sensitivity ranging between 89% and 100%).…”
Section: Discussionmentioning
confidence: 92%