The Stirling engine is a device that allows conversion of thermal energy into mechanical energy with relatively high efficiency. Existing commercial designs are mainly based on the usage of high temperature heat sources, whose availability from renewable or waste heat sources is significantly lower than that of moderate temperature sources. The paper presents the results of experimental research on a prototype alpha type Stirling engine powered by a moderate temperature source of heat. Obtained results enabled calibration of the evaluated theoretical model of the Stirling engine. The model of the engine has been subsequently used for the analysis of regenerator effectiveness influenced by the charge pressure and the heating temperature. Performed study allowed to determine further development directions of the prototype engine to improve its power and efficiency. As a result of optimization, worked out design will potentially increase the indicated efficiency up to 19.5% (5.5% prototype) and the indicated power up to 369 W (114 W prototype).