Caveolin-1 in Melanoma Progression

Lobos-González, Lorena; Aguilar, Lorena; Fernández, Gonzalo; Sanhueza, Carlos; Quest, Andrew F. G.

doi:10.5772/21829

Cited by 2 publications

(3 citation statements)

References 136 publications

(166 reference statements)

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“…We observed a stronger immunoreaction of caveolin-1 protein in the IGR-39 cell line derived from primary tumour of cutaneous origin, consistent with many previous reports indicating that the caveolin-1 level is elevated at the beginning of the disease in comparison to melanocytes and metastatic cells (FELICETTI et al 2009;LOBOS-GONZÁLEZ et al 2011). Two of the analysed cutaneous cell lines, namely WM-115 and WM-266-4, were derived from primary and metastatic sites, respectively, and originated from the same patient.…”

Section: Resultssupporting

confidence: 92%

“…Interestingly, in our study immunoblot analysis revealed that caveolin-1 expression was lower in mel-202 and 92-1 cell lines as compared to cutaneous cell lines. These results were inconsistent with the theory that caveolin-1 expression is elevated in primary melanoma cell lines (FELICETTI et al 2009;LOBOS-GONZÁLEZ et al 2011), however, this relates to cells of cutaneous origin. On the other hand, LOBOS-GONZÁLEZ et al (2014) have shown that caveolin-1 expression is lowest in melanocytes and increases with progression of human cutaneous melanoma.…”

Section: Resultscontrasting

confidence: 92%

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Expression of Caveolin-1 in Human Cutaneous and Uveal Melanoma Cells

Belkot

Bubka

Lityń́ska

2016

folia biol (krakow)

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BE£KOT K., BUBKA M., LITYÑSKA A. 2016. Expression of caveolin-1 in human cutaneous and uveal melanoma cells. Folia Biologica (Kraków) 64: 145-151.Caveolin-1 can act as a tumour promoter or suppressor depending on the cancer type and stage. In melanoma, information available concerning its expression is ambiguous. In this study, we investigated caveolin-1 mRNA and protein expression levels in human melanoma cell lines of different origin and progression stages. Metastatic cutaneous (WM-266-4, A375), primary cutaneous (WM-115, IGR-39) and primary uveal (mel-202, 92-1) cells were used for quantitative RT-PCR, Western blotting and confocal microscopy. We observed significantly higher expression of caveolin-1 mRNA in cutaneous than in uveal melanoma cells. In accordance, immunostaining of caveolin-1 was stronger in cutaneous cell extracts, while protein bands of uveal origin displayed weak signals. Finally, we detected differences in the caveolin-1 subcellular pattern of distribution between primary and metastatic cells. Overall, this is the first demonstration of caveolin-1 expression in human primary uveal melanoma cell lines and observation that the origin of cells (uveal/cutaneous) has an impact when considering the utility of caveolin-1 as a melanoma cell marker.

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Section: Resultssupporting

confidence: 92%

Section: Resultscontrasting

confidence: 92%

Expression of Caveolin-1 in Human Cutaneous and Uveal Melanoma Cells

Belkot

Bubka

Lityń́ska

2016

folia biol (krakow)

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“…In melanoma, CAV1 function is still ambiguous (17). Some studies associate CAV1 secreted in microvesicles with tumorigenicity (18), and others present CAV1 as a tumor suppressor by inhibiting Wnt-␤-catenin-Tcf/Lef (19), Src/FAK (20) pathways, or attenuating tumor cell motility by disrupting glycosphingolipid GD3-mediated malignant signaling (21).…”

mentioning

confidence: 99%

Cross-talk between Dopachrome Tautomerase and Caveolin-1 Is Melanoma Cell Phenotype-specific and Potentially Involved in Tumor Progression

Popa

Milac

Sima

et al. 2016

Journal of Biological Chemistry

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L-Dopachrome tautomerase (L-DCT), also called tyrosinaserelated protein-2 (TRP-2), is a melanoma antigen overexpressed in most chemo-/radiotherapeutic stress-resistant tumor clones, and caveolin-1 (CAV1) is a main regulator of numerous signaling processes. A structural and functional relationship between DCT and CAV1 is first presented here in two human amelanotic melanoma cell lines, derived from vertical growth phase (MelJuSo) and metastatic (SKMel28) melanomas. DCT co-localizes at the plasma membrane with CAV1 and Cavin-1, another molecular marker for caveolae in both cell phenotypes. Our novel structural model proposed for the DCT-CAV1 complex, in addition to co-immunoprecipitation and mass spectrometry data, indicates a possible direct interaction between DCT and CAV1. The CAV1 control on DCT gene expression, DCT posttranslational processing, and subcellular distribution is cell phenotype-dependent. DCT is a modulator of CAV1 stability and supramolecular assembly in both cell phenotypes. During autocrine stimulation, the expressions of DCT and CAV1 are oppositely regulated; DCT increases while CAV1 decreases. Sub-confluent MelJuSo clones DCT high /CAV1 low are proliferating and acquire fibroblast-like morphology, forming massive, confluent clusters as demonstrated by immunofluorescent staining and TissueFAXS quantitative image cytometry analysis. CAV1 down-regulation directly contributes to the expansion of MelJuSo DCT high subtype. CAV1 involved in the perpetuation of cell phenotype-overexpressing anti-stress DCT molecule supports the concept that CAV1 functions as a tumor suppressor in early stages of melanoma. DCT is a regulator of the CAV1-associated structures and is possibly a new molecular player in CAV1-mediated processes in melanoma.Cutaneous malignant melanoma remains the deadliest form of skin cancer worldwide (1). The skin melanocytes, normally located at the epidermis-dermis junction, start an uncontrolled proliferation process (radial growth phase, RGP), followed by dermis invasion (vertical growth phase, VGP).3 In a more advanced stage, melanoma cells migrate from this primary site to distant organs (liver, lung), populating them (metastasis) and eventually causing death (2). The mechanisms that specifically operate in tumor cell phenotypes determining the switch from the non-metastatic to metastatic stage are not entirely elucidated.Tyrosinase-related protein-2 (TRP-2 or Tyrp2) is a member of the tyrosinase-related protein family, together with tyrosinase (TYR) and tyrosinase-related protein-1 (TRP-1). Tyrosinase-related proteins are expressed by both melanocytes and melanoma forming the enzymatic cluster responsible for pigment production (3).TRP-2, also named dopachrome tautomerase (DCT), functions downstream of TYR by converting dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA), the precursor of eumelanins with a potent hydroxyl radical-scavenging activity (4). In the absence of DCT, dopachrome is spontaneously converted into 5,6-dihydroxyindole, a more toxic intermediate than...

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Caveolin-1 in Melanoma Progression

Cited by 2 publications

References 136 publications

Expression of Caveolin-1 in Human Cutaneous and Uveal Melanoma Cells

Expression of Caveolin-1 in Human Cutaneous and Uveal Melanoma Cells

Cross-talk between Dopachrome Tautomerase and Caveolin-1 Is Melanoma Cell Phenotype-specific and Potentially Involved in Tumor Progression

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