CD11c+ M1-like macrophages (MΦs) but not CD206+ M2-like MΦ are involved in folliculogenesis in mice ovary

Abstract

Macrophages (MΦs) are involved in folliculogenesis and ovulation. However, it is unknown which type of MΦ, M1 or M2, plays a more essential role in the ovary. CD206 or CD11c diphtheria toxin receptor transgenic (DTR) mice, which enable depletion of CD206+ M2 MΦs and CD11c+ MΦ or CD11c+ Dendritic cells (DCs), respectively, were used. Oocytes were used for in vitro fertilization and embryo transfer. In vitro fertilized embryos derived from M2 MΦ depleted oocytes were transferred to pseudo pregnant wild type mice. CD11c DTR mice were also used to investigate the role of CD11c cells, M1 MΦ and DCs in folliculogenesis. In WT mice, the proportion of CD206+ M2-like MΦs was not increased in follicular induction, while that of CD11c+ M1-like MΦs was increased. In CD206 DTR mice, folliculogenesis was normal and the ovulation number, fertilization rate, and implantation rate were similar to those in WT mice. In CD11c DTR mice, folliculogenesis was impaired with ovarian hemorrhage and the staining of platelet derived growth factor-receptor β (PDGF-Rβ), a marker of pericytes, and CD34, a marker of endothelial cells, was reduced. CD11c+ cells, M1 MΦs or DCs, may be involved in folliculogenesis, while M2 MΦs are not involved in folliculogenesis.

Direct electrical stimulation enhances osteogenesis by inducing Bmp2 and Spp1 expressions from macrophages and preosteoblasts

“…J774A.1 murine macrophage and phenotypically heterogeneous MC3T3‐E1 murine preosteoblastic cell lines were supplied from The European Collection of Authenticated Cell Cultures and used as received. The phenotype of J774A.1 cells used in this study were 91.4% M1 (CD11c positive), 0.6% M2 (CD206 positive), and the rest were M0 (neither CD11c nor CD206 positive) (Ono et al, ; Y. Zhu et al, ). Further details are described in the Supporting Information.…”

Puffing of Turmeric (Curcuma longa L.) Enhances its Anti-Inflammatory Effects by Upregulating Macrophage Oxidative Phosphorylation

“…Following observation of anti-obesity effects of both T and PT, anti-inflammatory markers on bone marrow-derived macrophages (BMDM) were quantified by specific staining of fluorescence conjugated mAbs followed by flow cytometric analysis, as expressed by mean fluorescence intensity (MFI, arbitrary unit). The expression of CD11c, a well-known macrophage surface marker for pro-inflammatory M1 subsets [ 17 ], was significantly ( p < 0.05) increased in the HFD group ( Figure 5 A, 12,898 ± 2558) vs. the AIN-76A diet control (4929 ± 1212). The addition of turmeric (HFD + T, 9011 ± 1501) did not exhibit a significant difference to HFD only ( p > 0.05), where puffed turmeric (HFD + PT, 4656 ± 1498) significantly suppressed surface display of CD11c comparable to the AIN-76A diet group.…”

Overview of RAW264.7 for osteoclastogensis study: Phenotype and stimuli

“…44 However, there still lack evidence for rigid classification criteria of cell lines between various passages and it seems to be cell-linage dependent. 48 Similarly, genes in subpanel-2 and subpanel-3 also associated with macrophage activation including: CD86, HIF-1α, CD11a, CD18, CD206, CD200R, Glut1 (Glucose transporter 1) and Ly6c and TfR2, Arg1 and SCARA-5b (scavenger receptor class A member 5) respectively. Therefore, confirmation of RAW264.7 stability through phenotype study among is important for the proper further data interpretation.…”

“…[45][46][47] Otherwise, the CD11c and iNOS in subpanel-1 are demonstrated as highly expressed in pro-inflammatory macrophage stage (M1). 48 Similarly, genes in subpanel-2 and subpanel-3 also associated with macrophage activation including: CD86, HIF-1α, CD11a, CD18, CD206, CD200R, Glut1 (Glucose transporter 1) and Ly6c and TfR2, Arg1 and SCARA-5b (scavenger receptor class A member 5) respectively. 32 Interestingly, however, as an iNOS inhibitory genes, the Arg1 also highly expressed in another polarized macrophage stage: anti-inflammatory (M2) phenotype, 49,50 and its expression in RAW264.7 has be showed significantly higher in the passage of No.…”