CD14+ cell collection in non‐cytokine‐stimulated donors with the COM.TEC cell separator

Abstract

A pure CD 14+ cell product with low numbers of residual cells was obtained by the PBSC-LYM program, which could be useful for good manufacturing practice-conformed production within closed systems. The MNC program offers the collection of high CD 14+ cell yields with excellent CEs but also high residual PLT counts.

Automated CD14+ monocyte collection with the autoMNC program of the COM.TEC cell separator

“…Recently, white blood cell (WBC) collection with the COM.TEC cell separator (Fresenius HemoCare GmbH, Bad Homburg, Germany) was evaluated. We found an excellent collection efficiency (CE) of CD14+ monocytes 1 . Leukapheresis units usually contain between 1 × 10 9 and 3 × 10 9 monocytes, which depends on the processing time and the apheresis device used 1‐5 .…”

“…WBC collection with the standard MNC program of the COM.TEC apheresis machine (Software Version 03.00.04) was recently investigated. 1 The program works with the PL1 single-stage chamber. Eight optical ports control the interface position within the separation chamber.…”

“…We found an excellent collection efficiency (CE) of CD14+ monocytes 1 . Leukapheresis units usually contain between 1 × 10 9 and 3 × 10 9 monocytes, which depends on the processing time and the apheresis device used 1‐5 . The standard MNC program of the COM.TEC cell separator, however, also collected large yields of residual platelets (PLTs) and red blood cells (RBCs) 1 .…”

“…1 Leukapheresis units usually contain between 1 ¥ 10 9 and 3 ¥ 10 9 monocytes, which depends on the processing time and the apheresis device used. [1][2][3][4][5] The standard MNC program of the COM.TEC cell separator, however, also collected large yields of residual platelets (PLTs) and red blood cells (RBCs). 1 Del Fante and coworkers 6 reported that the autoMNC program produced large buffy-coat (BC) volume with high RBC contamination.…”

“…[1][2][3][4][5] The standard MNC program of the COM.TEC cell separator, however, also collected large yields of residual platelets (PLTs) and red blood cells (RBCs). 1 Del Fante and coworkers 6 reported that the autoMNC program produced large buffy-coat (BC) volume with high RBC contamination. The contamination of WBC harvests with residual cells may also cause cell loss in blood donors.…”