CD44 Loss Disrupts Lung Lipid Surfactant Homeostasis and Exacerbates Oxidized Lipid-Induced Lung Inflammation

Abstract

Alveolar macrophages (AMs) are CD44 expressing cells that reside in the alveolar space where they maintain lung homeostasis by serving critical roles in immunosurveillance and lipid surfactant catabolism. AMs lacking CD44 are unable to bind the glycosaminoglycan, hyaluronan, which compromises their survival and leads to reduced numbers of AMs in the lung. Using RNA sequencing, lipidomics and multiparameter flow cytometry, we demonstrate that CD44 −/− mice have impaired AM lipid homeostasis and increased surfactant lipids in the lung. CD44 −/− AMs had increased expression of CD36, a lipid scavenger receptor, as well as increased intracellular lipid droplets, giving them a foamy appearance. RNA sequencing revealed the differential expression of genes associated with lipid efflux and metabolism in CD44 −/− AMs. Lipidomic analysis showed increased lipids in both the supernatant and cell pellet extracted from the bronchoalveolar lavage of CD44 −/− mice. Phosphatidylcholine species, cholesterol, oxidized phospholipids and levels of reactive oxygen species (ROS) were increased in CD44 −/− AMs. Oxidized phospholipids were more cytotoxic to CD44 −/− AMs and induced greater lung inflammation in CD44 −/− mice. Reconstitution of CD44 +/+ mice with CD44 −/− bone marrow as well as adoptive transfer of CD44 −/− AMs into CD44 +/+ mice showed that lipid accumulation in CD44 −/− AMs occurred irrespective of the lung environment, suggesting a cell intrinsic defect. Administration of colony stimulating factor 2 (CSF-2), a critical factor in AM development and maintenance, increased AM numbers in CD44 −/− mice and decreased phosphatidylcholine levels in the bronchoalveolar lavage, but was unable to decrease intracellular lipid accumulation in CD44 −/− AMs. Peroxisome proliferator-activated receptor gamma (PPARγ), downstream of CSF-2 signaling and a regulator of lipid metabolism, was reduced in the nucleus of CD44 −/− AMs, and PPARγ inhibition in normal AMs increased their lipid droplets. Thus, CD44 deficiency causes defects in AMs that lead to abnormal lipid accumulation and oxidation, which exacerbates oxidized lipid-induced lung inflammation. Collectively, these findings implicate CD44 as a regulator of lung homeostasis and inflammation.

The membrane receptor CD44: novel insights into metabolism

“…Studies show that CD44 deficiency increased lipid accumulation in alveolar macrophages (AMs) and increased surfactant lipids, resulting in impaired lung lipid homeostasis [57]. Transcriptome data from CD44-deficient AMs revealed that cd44 ablation affected lipid homeostasis through a variety of pathways, including sterol/cholesterol synthesis and protein phosphorylation-related signalling pathways.…”

On Top of the Alveolar Epithelium: Surfactant and the Glycocalyx

“…Alveolar macrophages express the hyaluronan receptor CD44 and thus are coated with bound hyaluronan promoting their survival [50]. Moreover, alveolar macrophages lacking CD44 have impaired lipid homeostasis leading to an increase in intraalveolar surfactant lipids [51]. This indicates an important role for hyaluronan in surfactant catabolism.…”

Metabolic Glycoengineering Enables the Ultrastructural Visualization of Sialic Acids in the Glycocalyx of the Alveolar Epithelial Cell Line hAELVi

“…We regularly observed these structures to be in direct contact with the glycocalyx on top of the plasma membrane. Previous work studying interactions between surfactant and specific glycocalyx components has been limited to the proteoglycan hyaluronan ( Lu et al, 2005 ; Taeusch et al, 2005 ; Wang et al, 2006 ; Lopez-Rodriguez et al, 2013 ; Dong et al, 2020 ). By combining MGE and EM, we found the extracellular lamellar structures to be enriched in sialic acids ( Figure 5 ).…”