CD44 MicroBeads accelerate HIV-1 infection in T cells

Terry, Valeri H.; Johnston, Ian; Spina, Celsa A.

doi:10.1016/j.virol.2009.03.022

Cited by 17 publications

(21 citation statements)

References 59 publications

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“…Primary CD4 + T cells were isolated from peripheral blood obtained from Stanford Blood Bank (Palo Alto, CA) using RosetteSep ™ Human CD4 + T Cell Enrichment Cocktail from STEMCELL ™ Technologies and Ficoll as described (Terry et al, 2009). Once isolated, cells were either cultured as described (Terry et al, 2009) or frozen in 10% DMSO, 90% culture media at a density of 10 7 per mL.…”

Section: Methodsmentioning

confidence: 99%

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A Hardwired HIV Latency Program

Razooky

Pai

Aull

et al. 2015

Cell

215

272

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SUMMARY Biological circuits can be controlled by two general schemes: environmental sensing or autonomous programs. For viruses such as HIV, the prevailing hypothesis is that latent infection is controlled by cellular state (i.e. environment) with latency simply an epiphenomenon of infected cells transitioning from an activated to resting state. However, we find HIV expression persists despite the activated-to-resting cellular transition. Mathematical modeling indicates that HIV’s Tat positive-feedback circuitry enables this persistence and strongly controls latency. To overcome the inherent crosstalk between viral circuitry and cellular activation, and directly test this hypothesis, we synthetically decouple viral dependence on cellular environment from viral transcription. These circuits enable control of viral transcription without cellular activation and show that Tat feedback is sufficient to regulate latency independent of cellular activation. Overall, synthetic reconstruction demonstrates that a largely autonomous, viral-encoded program underlies HIV latency—potentially explaining why cell-targeted latency-reversing agents exhibit incomplete penetrance.

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Section: Methodsmentioning

confidence: 99%

“…Once isolated, cells were either cultured as described (Terry et al, 2009) or frozen in 10% DMSO, 90% culture media at a density of 10 7 per mL. For infections, primary CD4 + T cells were pre-activated for 2–3 days with αCD3/CD28 beads (Dynabeads ™ , Life Technologies) as per manufacturer instructions.…”

Section: Methodsmentioning

confidence: 99%

A Hardwired HIV Latency Program

Razooky

Pai

Aull

et al. 2015

Cell

215

272

View full text Add to dashboard Cite

show abstract

“…In biomedical research, cell purification has been widely used for many different purposes, including tissue engineering and regenerative medicine, cancer therapy, and research on the pathogenesis of infectious diseases [22,23,24]. Overall, this is reflected in more than 40,000 publications, many of which include high-impact scientific papers, with a progressive increase in the number of citations per year (fig.…”

Section: Cell Purification Technologiesmentioning

confidence: 99%

Cell Purification: A New Challenge for Biobanks

Almeida¹,

García‐Montero²,

Órfão³

2014

Pathobiology

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Performing ‘-omics' analyses on heterogeneous biological tissue samples, such as blood or bone marrow, can lead to biased or even erroneous results, particularly when the targeted cells and/or molecules are present at relatively low percentages/amounts. In such cases, whole sample analysis will most probably dilute and mask the features of the cell and/or molecules of interest, and this will negatively impact the results and their interpretation. Therefore, frequently it is critically important to have well-characterized and high-quality purified cell populations for the reliable detection of subtle variations in their specific features, such as gene expression profile, protein expression pattern and metabolic status. Biobanks are technological platforms which aim to provide researchers access to a large number of high-quality biological samples and their associated data, particularly to support high-quality scientific and clinical research projects, and such projects will benefit enormously by having access to high-quality purified cell populations or their biological components (e.g. DNA, RNA, proteins). Therefore, a clear opportunity exists for preparative cell sorting techniques in biobanks. Although multiple different cell purification approaches exist or are under development (e.g. cell purification techniques based on cell adherence, density and/or cell size properties, methods based on antibody binding as well as new lab-on-a-chip purification techniques), the choice for a specific technology depends on multiple variables, including cell recovery, purity and yield, among others. In addition, most cell purification approaches are not well suited for high-throughput (HT) purification of multiple cell populations coexisting in a sample. Here we review the most (currently) used cell sorting methods that may be applied for sample preparation in biobanks. For the different approaches, technical considerations about their advantages and limitations are highlighted, and the requirements to be met by a HT cell sorting technology to be used in biobanks are also discussed.

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“…The scale of microfluidic systems and their resemblance to 3D physiological environments provides a compatible interface for manipulating cells and imitating in vivo conditions. The ability to sort cells enables important discoveries in cell biology, thus further enabling diverse biomedical applications in a variety of areas including HIV diagnostics (2) and cancer therapy (3).…”

Section: Introductionmentioning

confidence: 99%

Large-Volume Microfluidic Cell Sorting for Biomedical Applications

Warkiani

Wu²,

Tay

et al. 2015

Annu. Rev. Biomed. Eng.

104

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Microfluidic cell-separation technologies have been studied for almost two decades, but the limited throughput has restricted their impact and range of application. Recent advances in microfluidics enable high-throughput cell sorting and separation, and this has led to various novel diagnostic and therapeutic applications that previously had been impossible to implement using microfluidics technologies. In this review, we focus on recent progress made in engineering large-volume microfluidic cell-sorting methods and the new applications enabled by them.

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CD44 MicroBeads accelerate HIV-1 infection in T cells

Cited by 17 publications

References 59 publications

A Hardwired HIV Latency Program

A Hardwired HIV Latency Program

Cell Purification: A New Challenge for Biobanks

Large-Volume Microfluidic Cell Sorting for Biomedical Applications

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