Large-Volume Microfluidic Cell Sorting for Biomedical Applications

Warkiani, Majid Ebrahimi; Wu, Lidan; Tay, Andy; Han, Jongyoon

doi:10.1146/annurev-bioeng-071114-040818

Cited by 104 publications

(85 citation statements)

References 174 publications

(156 reference statements)

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“…Biomaterials are engineered to take a form that can work alone or as part of a complex system providing direction to the course of any therapeutic procedure by regulating interactions with components of living systems [1,2]. Biomaterials are used to restore or augment the physiological function of diseased or damaged soft and hard tissues via replacement or regeneration [3,4,5,6].…”

Section: Introductionmentioning

confidence: 99%

Macrophages, Foreign Body Giant Cells and Their Response to Implantable Biomaterials

et al. 2015

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All biomaterials, when implanted in vivo, elicit cellular and tissue responses. These responses include the inflammatory and wound healing responses, foreign body reactions, and fibrous encapsulation of the implanted materials. Macrophages are myeloid immune cells that are tactically situated throughout the tissues, where they ingest and degrade dead cells and foreign materials in addition to orchestrating inflammatory processes. Macrophages and their fused morphologic variants, the multinucleated giant cells, which include the foreign body giant cells (FBGCs) are the dominant early responders to biomaterial implantation and remain at biomaterial-tissue interfaces for the lifetime of the device. An essential aspect of macrophage function in the body is to mediate degradation of bio-resorbable materials including bone through extracellular degradation and phagocytosis. Biomaterial surface properties play a crucial role in modulating the foreign body reaction in the first couple of weeks following implantation. The foreign body reaction may impact biocompatibility of implantation devices and may considerably impact short- and long-term success in tissue engineering and regenerative medicine, necessitating a clear understanding of the foreign body reaction to different implantation materials. The focus of this review article is on the interactions of macrophages and foreign body giant cells with biomaterial surfaces, and the physical, chemical and morphological characteristics of biomaterial surfaces that play a role in regulating the foreign body response. Events in the foreign body response include protein adsorption, adhesion of monocytes/macrophages, fusion to form FBGCs, and the consequent modification of the biomaterial surface. The effect of physico-chemical cues on macrophages is not well known and there is a complex interplay between biomaterial properties and those that result from interactions with the local environment. By having a better understanding of the role of macrophages in the tissue healing processes, especially in events that follow biomaterial implantation, we can design novel biomaterials-based tissue-engineered constructs that elicit a favorable immune response upon implantation and perform for their intended applications.

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Section: Introductionmentioning

confidence: 99%

Macrophages, Foreign Body Giant Cells and Their Response to Implantable Biomaterials

et al. 2015

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“…[17][18][19] When particles flow in a confined microchannel, they become focused into specific positions along the channel due to sizedependent hydrodynamic forces. [17][18][19] Inertial microfluidics supports high volumetric flow rates (>mL per minute per channel 20 ), and the throughput can be further increased (as high as 1 L min −1 ) using multiple devices in parallel. [21][22][23] Moreover, sorting yeast and mammalian cells at high cell concentrations in microchannels can be performed without additional buffer flows.…”

Section: Introductionmentioning

confidence: 99%

Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics

et al. 2018

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Removing nonviable cells from a cell suspension is crucial in biotechnology and biomanufacturing. Labelfree microfluidic cell separation devices based on dielectrophoresis, acoustophoresis, and deterministic lateral displacement are used to remove nonviable cells. However, their volumetric throughputs and test cell concentrations are generally too low to be useful in typical bioreactors in biomanufacturing. In this study, we demonstrate the efficient removal of small (<10 μm) nonviable cells from bioreactors while maintaining viable cells using inertial microfluidic cell sorting devices and characterize their performance. Despite the size overlap between viable and nonviable cell populations, the devices demonstrated 3.5-28.0% dead cell removal efficiency with 88.3-83.6% removal purity as well as 97.8-99.8% live cell retention efficiency at 4 million cells per mL with 80% viability. Cascaded and parallel configurations increased the cell concentration capacity (10 million cells per mL) and volumetric throughput (6-8 mL min −1). The system can be used for the removal of small nonviable cells from a cell suspension during continuous perfusion cell culture operations.

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“…Recently, precision medicine becomes one of the frontier research fields where fast, reliable, high-throughput and low-cost technologies will take place. In this area, dielectrophoretic tools will be widely used in separation, characterization and manipulation of clinical samples thanks to its label-free and quantitative nature [8,12,17,23].…”

Section: Discussionmentioning

confidence: 99%

A Numerical Approach for Dielectrophoretic Characterization and Separation of Human Hematopoietic Cells

Erdem¹,

Yıldızhan²,

Elitaş³

2017

IJERT

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Abstract-Rapidand reliable characterization of hematopoietic cells still remain the first step for precise medicine. Diagnosis of various diseases, ranging from infectious to cancer, relies on quantification of hematopoietic cells from blood. Therefore, there is an emerging need for label-free, lowcost, time-efficient, reproducible and quantitative characterization tools for the blood cells. Addressed herein is a numerical analysis for dielectrophoretic characterization of red blood cells, T-lymphocytes, B-lymphocytes and monocytes, which quantitatively incorporate with the membrane features of these cells to provide more insight into their dielectrophoretic responses.

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Large-Volume Microfluidic Cell Sorting for Biomedical Applications

Cited by 104 publications

References 174 publications

Macrophages, Foreign Body Giant Cells and Their Response to Implantable Biomaterials

Macrophages, Foreign Body Giant Cells and Their Response to Implantable Biomaterials

Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics

A Numerical Approach for Dielectrophoretic Characterization and Separation of Human Hematopoietic Cells

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