CD9 Expression in Solid Non-neuroepithelial Tumors and Infiltrative Astrocytic Tumors

Kawashima, Masatou; Doh‐ura, Katsumi; Mekada, Eisuke; Fukui, Masashi; Iwaki, Toru

doi:10.1177/002215540205000906

Cited by 18 publications

(15 citation statements)

References 29 publications

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“…2a). In contrast, expression of CD9 in human cerebral white matter was higher than in glioma cell lines A172 (p = 0.003) and U343MG (p = 0.004), presumably reflecting high amounts of CD9 localized mainly along the outermost membrane of compact myelin [20] (Fig. 2a).…”

Section: Cd9 Is Expressed In Glioma Cell Lines and Glioblastoma Specimentioning

confidence: 88%

Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma

et al. 2015

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Platelet-derived growth factor receptor (PDGFR) signaling plays an important role in the biology of malignant gliomas. To investigate mechanisms modulating PDGFR signaling in gliomagenesis, we employed a Drosophila glioma model and genetic screen to identify genes interacting with Pvr, the fly homolog of PDGFRs. Glial expression of constitutively activated Pvr (λPvr) led to glial over migration and lethality at late larval stage. Among 3316 dsRNA strains crossed against the tester strain, 128 genes shifted lethality to pupal stage, including tetraspanin 2A (tsp2A). In a second step knockdown of all Drosophila tetraspanins was investigated. Of all tetraspanin dsRNA strains only knockdown of tsp2A partially rescued the Pvr-induced phenotype. Human CD9 (TSPAN29/MRP-1), a close homolog of tsp2A, was found to be expressed in glioma cell lines A172 and U343MG as well as in the majority of glioblastoma samples (16/22, 73 %). Furthermore, in situ proximity ligation assay revealed close association of CD9 with PDGFR α and β. In U343MG cells, knockdown of CD9 blocked PDGF-BB stimulated migration. In conclusion, modulation of PDGFR signaling by CD9 is evolutionarily conserved from Drosophila glia to human glioma and plays a role in glia migration.

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Section: Cd9 Is Expressed In Glioma Cell Lines and Glioblastoma Specimentioning

confidence: 88%

Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma

et al. 2015

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“…However, there are two investigations in the literature describing both the cytoplasmic and membranous patterns, as noted in our study in addition to the usual membranous staining. The first was by Kawashima et al (2002) and the second was by Houle et al (2002)). In the former, CD9 expression was evaluated in different types of brain tumours, and they concluded that CD9 expression in astrocytic tumours correlated with their malignancy and thus CD9 protein may have a different role in brain tumours than solid tumours elsewhere.…”

Section: Discussionmentioning

confidence: 99%

Motility-related protein-1 (MRP-1/CD9) expression can predict disease-free survival in patients with squamous cell carcinoma of the head and neck

Mhawech¹,

Dulguerov²,

Tschanz³

et al. 2004

Br J Cancer

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CD9 is a transmembrane protein that has been implicated in cell adhesion, motility and proliferation, and numerous studies have demonstrated the prognostic value of its expression in different solid tumours. The purpose of this study is to determine the predictive value of CD9 in squamous cell carcinoma (SCC) of the head and neck. A total of 153 cases were examined for CD9 expression using immunohistochemistry applied on formalin-fixed, paraffin-embedded tissue. Cases were stratified in two categories depending on CD9 expression, as positive (X50% positive cells) or reduced (o50%). In all, 108 cases were positive for CD9 (85 cases with membranous, and 23 with both membranous and cytoplasmic staining) and 45 reduced expression. Reduced CD9 expression was significantly associated with high grade (P ¼ 0.0007) and lower disease-free survival (DFS) (P ¼ 0.017). The latter retained its significance in the multivariate analysis. When the 23 cases with both membranous and cytoplasmic patterns were studied as a separate subgroup, there were significant associations between CD9 expression and tumour grade (P ¼ 0.025) (95% CI 11 -68), tumour stage (P ¼ 0.08) (95% CI 3.5 -86) and the occurrence of any failure (P ¼ 0.083) (95% CI À1.7 -57). Immunohistochemical CD9 expression proved to be an independent prognostic factor in SCC of the head and neck, and it may detect patients at a high risk of recurrence. In addition, the cytoplasmic pattern seems to have an even more significant value. However, this finding is limited to the small number of cases with this pattern.

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“…Moreover, CD9 overexpression has been reported in aggressive gastric carcinomas (Hori et al, 2004) and high-grade astrocytic tumors (Kawashima et al, 2002). Therefore, CD9 could be required for the invasive growth of TGCTs.…”

Section: Role Of Ddx1 In Testicular Tumorigenesismentioning

confidence: 99%

DDX1 is required for testicular tumorigenesis, partially through the transcriptional activation of 12p stem cell genes

et al. 2009

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Cytogenetic analysis has identified 12p gain as the most frequent abnormality in human testicular germ cell tumors (TGCTs). It has been suggested that amplification and overexpression of stem cell-associated genes, including cyclin-D2, on the human chromosome 12p region are involved in germ cell tumorigenesis. By subtractive cDNA analysis, we identified Ddx1, a member of the DEAD-box protein family, as a gene predominantly expressed in the primordial germ cells of mouse embryos. Knockdown of Ddx1 in a mouse spermatogonia-derived cell line, GC-1spg, by short interference RNA repressed the expression of cyclin-D2, CD9 and GDF3 genes. In the mouse cyclin-D2 gene, a genomic DNA region between À348 and À329 was responsible for transcriptional activation by DDX1 based on reporter and gel shift assays. Similarly, DDX1 knockdown in the human TGCT cell line NEC8 repressed the expression of stem cell-associated genes localized on chromosome 12p13.3, including cyclin-D2, CD9 and NANOG. DDX1-knockeddown TGCT cells could not form solid tumors in nude mice. Furthermore, in situ hybridization revealed that DDX1 mRNA was produced in both seminoma and nonseminoma types of human TGCT samples. We conclude that DDX1 is a critical factor for testicular tumorigenesis.

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CD9 Expression in Solid Non-neuroepithelial Tumors and Infiltrative Astrocytic Tumors

Cited by 18 publications

References 29 publications

Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma

Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma

Motility-related protein-1 (MRP-1/CD9) expression can predict disease-free survival in patients with squamous cell carcinoma of the head and neck

DDX1 is required for testicular tumorigenesis, partially through the transcriptional activation of 12p stem cell genes

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