CDH13 and FLBN3 Gene Methylation are Associated with Poor Prognosis in Colorectal Cancer

Abstract

The aim of this study was to identify potential epigenetic prognostic biomarkers for colorectal cancer (CRC) in the Chinese population. The methylation status of five tumor suppressor genes (CDH13, DLEC1, FBLN3, hMHL1 and RUNX3) was determined using manual microdissection followed by methylation-specific PCR in 85 paired CRC specimens and adjacent normal tissue. The results showed that methylation frequencies in cancerous tissues were 31.8% for CDH13, 37.6% for DLEC1, 38.8% for FBLN3, 22.4% for hMHL1 and 27.1% for RUNX3, all of which were significantly higher than in corresponding normal tissue. Furthermore, CDH13 methylation was associated with poor differentiation (P = 0.019) and tended to be predominant in advanced stages (P = 0.084); FBLN3 methylation was associated with advanced stages (P = 0.027) and lymph node metastasis (P = 0.029). Accordingly, the methylation status of CDH13 (P = 0.022), FBLN3 (P = 0.008), CDH13 and/or FBLN3 (P = 0.001) predicted adverse overall survival in CRC, while hMHL1 methylation showed a protective role in survival (P = 0.046). Cox proportional hazard models further indicated that CDH13 and/or FBLN3 methylation, but not that of hMHL1, was an independent prognostic factor for CRC. In conclusion, we found CDH13 and FBLN3 gene methylation are potential biomarkers for poor prognosis in CRC.

Aberrant Methylation of T-cadherin Can Be a Diagnostic Biomarker for Colorectal Cancer

“…The characteristics of eligible studies are summarized in Table I. A total of 9 studies (11)(12)(13)(14)(15)(16)(17)(18)(19), including 488 cases with CRC, 48 premalignant tissues, 298 adjacent tissues, and 144 normal, met the inclusion criteria for this meta-analysis ( Figure 1). The total number of patients was 998.…”

Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK

“…e.g. methylation of p16 is a frequent and early event in ESCC [33], methylation of CDH13 is associated with high grade and advanced stage cancer [34, 35]. In vitro assays used in this study only reveal effects of silencing DNMT1 on methylation in one aspect, but cannot mimic the progression of ESCC.…”

An Integrative Analysis of Meningioma Tumors Reveals the Determinant Genes and Pathways of Malignant Transformation

“…Nevertheless, mQTL genes were also enriched in other cancer-related pathways. For example, five mQTL putative genes are part of the cadherin family, and at least two ( CHD12 and CHD13 ) have been broadly studied for their role in cancer (72–74). Genes related to angiogenesis and vascular permeability pathways had also significant matches, and at least one of them ( NRP1 ) has been studied before in meningioma (49).…”